US4493894A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Polysaccharide beads
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1980-09-16
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Granted: 1985-01-15
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Expires: 2000-09-16
Abstract
Abstract A matrix comprising a water-insoluble β-1,3-glucan gel in the shape of beads with diameters within the range of about 5 to 1000μ is prepared by, for example, dispersing an alkaline aqueous solution of a water-soluble β-1,3-glucan in a water-immiscible organic solvent, and adding an organic acid to the resultant dispersion. The matrix is useful as carrier materials for immobilized enzymes, affinity chromatography, gel filtration, ion exchange and other applications.
Inventors
- 👤 Yutaka Miyashiro
- 👤 Masao Ogawa
- 👤 Yoshio Yamazaki
- 👤 Seizi Igarasi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:04
CPC Classifications
C; C08; C08J; C08J3/00; C08J3/12; C08J3/14; B; B01; B01J; B01J13/00; B01J13/02; B01J20/00; B01J20/22; C08B; C08B37/00; C08B37/0006; C08B37/0024; C08J2305/00; Y; Y10; Y10T; Y10T428/00; Y10T428/26; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
47 words
In a chemical method comprising the employment of a polysaccharide matrix packed in a column the improvement according to which the matrix material is a water-insoluble β-1,3-glucan gel which is shaped in the form of beads with a diameter within the range of about 5 to 1000μ.
Claim 2
Dependent Claim
47 words
In a chemical method comprising the employment of a polysaccharide matrix packed in a column the improvement according to which the matrix material is a water-insoluble β-1,3-glucan gel which is shaped in the form of beads with a diameter within the range of about 5 to 1000μ.
Claim 3
Dependent Claim
26 words
A method according to claim 1 which is a method for the preparation of a carrier for immobilized enzymes, affinity chromatography, gel filtration or ion exchange.
Claim 4
Dependent Claim
20 words
A method according to claim 1 wherein the beads have a diameter within the range of about 30 to 300μ.
Claim 5
Dependent Claim
25 words
A method according to claim 1 wherein the matrix material is in the shape of beads coated with a water-insoluble β-1,3-glucan on a core material.
Claim 6
Dependent Claim
13 words
A method according to claim 4 wherein the core material is SIRASU bead.
Claim 7
Dependent Claim
14 words
A method according to claim 4 wherein the core material is spherically molded alumina.
Claim 8
Dependent Claim
13 words
A method according to claim 4 wherein the core material is glass bead.
Claim 9
Dependent Claim
12 words
A method according to claim 4 wherein the core material is pumice.
Claim 10
Dependent Claim
19 words
A method according to claim 3 wherein the beads have diameter within the range of about 30 to 150μ.
Claim 11
Dependent Claim
26 words
A method according to claim 1 wherein the water-insoluble β-1,3-glucan is produced by a microorganism belonging to the genus Alcaligenes, the genus Agrobacterium or Poria cocos.
Claim 12
Dependent Claim
12 words
A method according to claim 10 wherein the microorganism is Alcaligenes faecalis.
Claim 13
Dependent Claim
12 words
A method according to claim 10 wherein the microorganism is Agrobacterium radiobacter.
Claim 14
Dependent Claim
12 words
A method according to claim 10 wherein the microorganism is Poria cocos.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-15 | 1988-07-16 | 1989-01-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-15 | 1992-07-16 | 1993-01-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-15 | 1996-07-16 | 1997-01-15 | $7700.00 | Window Closed / Lapsed |