US4143201A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Polysaccharide beads
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1976-10-20
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Granted: 1979-03-06
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Expires: 1996-10-20
Abstract
Abstract A matrix comprising a water-insoluble β-1,3-glucan gel in the shape of beads with diameters within the range of about 5 to 1000 μ is prepared by, for example, dispersing an alkaline aqueous solution of a water-insoluble β-1,3-glucan in a water-immiscible organic solvent, and adding an organic acid to the resultant dispersion. The matrix is useful as carrier materials for immobilized enzymes, affinity chromatography, gel filtration, ion exchange and other applications.
Inventors
- 👤 Yutaka Miyashiro
- 👤 Masao Ogawa
- 👤 Yoshio Yamazaki
- 👤 Seizi Igarasi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:33
CPC Classifications
C; C08; C08J; C08J3/00; C08J3/12; C08J3/14; B; B01; B01J; B01J13/00; B01J13/02; B01J20/00; B01J20/22; C08B; C08B37/00; C08B37/0006; C08B37/0024; C08J2305/00; Y; Y10; Y10T; Y10T428/00; Y10T428/26; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
79 words
A carrier for immobilized enzyme, affinity chromatography, gel filtration and ion exchange comprising a water-insoluble β-1,3-glucan gel in the shape of beads having a diameter within the range of about 5 to 1000μ, said beads being composed of water-insoluble β-1,3-glucan gel surrounding a core material selected from the group consisting of (1) beads of pumice ejected from volcanoes and the secondary deposit of such pumice, (2) pumice, (3) alumina, (4) silica, (5) glass bead and (6) hollow glass bead.
Claim 2
Dependent Claim
79 words
A carrier for immobilized enzyme, affinity chromatography, gel filtration and ion exchange comprising a water-insoluble β-1,3-glucan gel in the shape of beads having a diameter within the range of about 5 to 1000μ, said beads being composed of water-insoluble β-1,3-glucan gel surrounding a core material selected from the group consisting of (1) beads of pumice ejected from volcanoes and the secondary deposit of such pumice, (2) pumice, (3) alumina, (4) silica, (5) glass bead and (6) hollow glass bead.
Claim 3
Dependent Claim
20 words
A carrier according to claim 1, wherein the beads have a diameter within the range of about 30 to 300μ.
Claim 4
Dependent Claim
24 words
A carrier according to claim 1, wherein the core material is beads of pumice ejected from volcanoes and the secondary deposit of such pumice.
Claim 5
Dependent Claim
14 words
A carrier according to claim 1, wherein the core material is spherically molded alumina.
Claim 6
Dependent Claim
13 words
A carrier according to claim 1, wherein the core material is glass bead.
Claim 7
Dependent Claim
12 words
A carrier according to claim 1, wherein the core material is pumice.
Claim 8
Dependent Claim
20 words
A carrier according to claim 1, wherein the beads have a diameter within the range of about 30 to 150μ.
Claim 9
Dependent Claim
25 words
A carrier according to claim 1, wherein the water-insoluble β-1,3-glucan is produced by microorganisms belonging to the genus Alcaligenes, the genus Agrobacterium or Poria cocos.
Claim 10
Dependent Claim
12 words
A carrier according to claim 8, wherein the microorganism is Alcaligenes faecalis.
Claim 11
Dependent Claim
12 words
A carrier according to claim 8, wherein the microorganism is Agrobacterium radiobacter.
Claim 12
Dependent Claim
12 words
A carrier according to claim 8, wherein the microorganism is Poria cocos.
References Cited (Backward Citations)
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US3754925A
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US3803045A
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US3899480A
Cited
US3933788A
Cited
US3915800A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-03-05 | 1982-09-04 | 1983-03-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-03-05 | 1986-09-04 | 1987-03-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-03-05 | 1990-09-04 | 1991-03-06 | $7700.00 | Window Closed / Lapsed |