US4482485A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of preparation of human urine origin colony-stimulating factor and kallikrein
Assignee: Japan Science and Technology Agency
•
Filing: 1984-01-04
•
Granted: 1984-11-13
•
Expires: 2004-01-04
Abstract
Abstract Human urine-origin colony-stimulating factor and kallikrein are separated in pure form, respectively, from an aqueous solution thereof, such as a urinary protein-concentrated solution by subjecting the solution added with a stabilizer such as octyl-phenoxypolyethoxyethanaol or polyethylene glycol both having a molecular weight of 1,000-10,000, to high-performance gel filtration effective in molecular exclusive limit of 10 5 -5×10 5 as determined with globular protein.
Inventors
- 👤 Satoshi Funakoshi
- 👤 Kazuo Morimoto
- 👤 Morio Kuboyama
- 👤 Nobuya Yanai
- 👤 Muneo Yamada
- 👤 Hajime Yokota
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6445; A; A61; A61K; A61K35/00; A61K35/12; A61K35/22; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
105 words
A method of preparation of human urine origin colony-stimulating factor and kallikrein, respectively, which comprises concentrating urine of normal humans or a solution containing human urine origin colony-stimulating factor and kallikrein with respect to proteins contained therein, equilibrating the resulting concentrated liquid with a buffer solution containing a stabilizer, subjecting the liquid to high-performance gel filtration chromatography by introducing the liquid into a column which has been filled with a gel having a molecular exclusive limit of 10 5 -×10 5 as determined with globular protein and equilibrated with said buffer solution to fractionating the colony-stimulating factor fractions and kallikrein fractions, and collecting the both.
Claim 2
Independent Claim
105 words
A method of preparation of human urine origin colony-stimulating factor and kallikrein, respectively, which comprises concentrating urine of normal humans or a solution containing human urine origin colony-stimulating factor and kallikrein with respect to proteins contained therein, equilibrating the resulting concentrated liquid with a buffer solution containing a stabilizer, subjecting the liquid to high-performance gel filtration chromatography by introducing the liquid into a column which has been filled with a gel having a molecular exclusive limit of 10 5 -×10 5 as determined with globular protein and equilibrated with said buffer solution to fractionating the colony-stimulating factor fractions and kallikrein fractions, and collecting the both.
Claim 3
Dependent Claim
30 words
A method for preparation according to claim 1, wherein said buffer solution has an ionic strength of 0.05-0.5 and a pH of 6.0-8.0, and contains 0.01-0.1% (w/v) of a stabilizer.
Claim 4
Dependent Claim
24 words
A method of preparation according to claim 1, wherein the stabilizer is octyl-phenoxypolyethoxyethanol or polyethylene glycol both having a molecular weight of 1,000-10,000 daltons.
Claim 5
Dependent Claim
23 words
A method of preparation according to claim 1, wherein the amount of the stabilizer contained is 0.01-0.1% (w/v) based on the buffer solution.
References Cited (Backward Citations)
US2874088A
Cited
US3100736A
Cited
US3905870A
Cited
US4275056A
Cited
US4252902A
Cited
US4289690A
Cited
US4178285A
Cited
US4342828A
Cited
US4393140A
Cited
US4359415A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-11-13 | 1988-05-14 | 1988-11-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-11-13 | 1992-05-14 | 1992-11-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-11-13 | 1996-05-14 | 1996-11-13 | $7700.00 | Window Closed / Lapsed |