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US4472572A
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Adenosine pyrophosphate polynucleotide compound
Assignee: INNOVAX LABORATORIES Ltd A CA LP
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Filing: 1982-02-01
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Granted: 1984-09-18
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Expires: 2002-02-01
Abstract
Abstract Composition and methods of production of the compound P1-adenosine, P2-(3' nucleotide monophosphate)-5' pyrophosphate (AppNp) are disclosed. AppNp is useful in the synthesis of a predetermined sequence of RNA using the enzyme T4 RNA ligase. The use of this invention in the field of recombinant DNA will greatly enhance the ability to synthesize genes of known composition.
Inventors
- 👤 Hiroaki Shizuya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:20
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/36
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
70 words
A compound P 1 -Adenosine, P 2 -(3-nucleotide monophosphate)-5' pyrophosphate having the formula: ##STR12## wherein the nucleotide is selected from the group consisting of adenine, guanine, uracil, hypoxanthine, thymidine, deoxyadenine, deoxycytosine, deoxyguanine, deoxyuridine, and deoxyhypoxanthine, whereby said compound may be used to enzymatically add said nucleotide monophosphate specifically to the 3' terminus of an oligonucleotide having a hydroxyl group on said 3' terminus, said addition thereby preventing subsequent continuous additions.
Claim 2
Dependent Claim
70 words
A compound P 1 -Adenosine, P 2 -(3-nucleotide monophosphate)-5' pyrophosphate having the formula: ##STR12## wherein the nucleotide is selected from the group consisting of adenine, guanine, uracil, hypoxanthine, thymidine, deoxyadenine, deoxycytosine, deoxyguanine, deoxyuridine, and deoxyhypoxanthine, whereby said compound may be used to enzymatically add said nucleotide monophosphate specifically to the 3' terminus of an oligonucleotide having a hydroxyl group on said 3' terminus, said addition thereby preventing subsequent continuous additions.
References Cited (Backward Citations)
FR2071360A5
Cited
US4328336A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-18 | 1988-03-19 | 1988-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-18 | 1992-03-19 | 1992-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-18 | 1996-03-19 | 1996-09-18 | $7700.00 | Window Closed / Lapsed |