Patent US4468466A successfully refreshed from Google Patents.
US4468466A
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Silver stains for protein in gels--a modified procedure
Assignee: United States Department of Health and Human Services
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Filing: 1982-02-16
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Granted: 1984-08-28
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Expires: 2002-02-16
Abstract
Abstract In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt and actuating irradiation, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
Inventors
- 👤 James H. Morrissey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/683; G01N33/6836
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
53 words
In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt and actuating irradiation, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
Claim 2
Dependent Claim
53 words
In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt and actuating irradiation, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
Claim 3
Dependent Claim
41 words
The improvement of claim 1 wherein the dithiothreitol is used in a concentration of from about 2.0 to about 10.0 μg/ml and the protein in gel being treated is soaked therein for a time of about 15 to about 45 minutes.
Claim 4
Dependent Claim
22 words
The improvement of claim 2 wherein the concentration is from 4.0 to 6.0 μg/ml and the time is 25 to 35 minutes.
Claim 5
Dependent Claim
19 words
The improvement of claim 3 wherein the concentration is about 5.0 μg/ml and the time is about 30 minutes.
Claim 6
Dependent Claim
50 words
In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
Claim 7
Dependent Claim
50 words
In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
Claim 8
Dependent Claim
41 words
The improvement of claim 5 wherein the dithiothreitol is used in a concentration of from about 2.0 to about 10.0 μg/ml and the protein in gel being treated is soaked therein for a time of about 15 to about 45 minutes.
Claim 9
Dependent Claim
22 words
The improvement of claim 6 wherein the concentration is from 4.0 to 6.0 μg/ml and the time is 25 to 35 minutes.
Claim 10
Dependent Claim
19 words
The improvement of claim 7 wherein the concentration is about 5.0 μg/ml and the time is about 30 minutes.
References Cited (Backward Citations)
US4405720A
Cited
US4416998A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-28 | 1988-02-27 | 1988-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-28 | 1992-02-27 | 1992-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-28 | 1996-02-27 | 1996-08-28 | $7700.00 | Window Closed / Lapsed |