US4416998A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Silver stain procedure and kit for same
Assignee: Health Products Inc
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Filing: 1982-03-17
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Granted: 1983-11-22
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Expires: 2002-03-17
Abstract
Abstract A silver stain procedure wherein a substance capable of binding silver is treated with a glutaraldehyde solution, an aqueous silver salt solution, a reducing solution and an aqueous carbonate or sulfate solution and kit useful in practicing same.
Inventors
- 👤 Lonnie D. Adams
- 👤 David W. Sammons
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 19:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/683; G01N33/6836; G01N33/52; G01N1/00; G01N1/28; G01N1/30; G01N2001/305; Y; Y10; Y10T; Y10T436/00; Y10T436/25
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
66 words
A method for staining a substance which is capable of binding silver which is supported in a matrix which comprises the steps of treating said substance with a 2.5-25% glutaraldehyde solution, washing said substance with water, equilibrating said substance in an aqueous silver salt solution, subjecting said substance to a reducing solution, and immersing said substance in multiple and sequential aqueous carbonate or sulfate salt solutions.
Claim 2
Independent Claim
66 words
A method for staining a substance which is capable of binding silver which is supported in a matrix which comprises the steps of treating said substance with a 2.5-25% glutaraldehyde solution, washing said substance with water, equilibrating said substance in an aqueous silver salt solution, subjecting said substance to a reducing solution, and immersing said substance in multiple and sequential aqueous carbonate or sulfate salt solutions.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein said substance is protein, a polypeptide or a protein containing substance.
Claim 4
Independent Claim
23 words
The method of claims 1 or 2 wherein said substance is subjected to fixation and washing prior to treatment with the glutaraldehyde solution.
Claim 5
Dependent Claim
18 words
The method of claim 3 wherein the fixation and washing solution comprises a 12.5% solution of trichloroacetic acid.
Claim 6
Independent Claim
13 words
The method of claims 1 or 2 wherein the matrix is polyacrylamide gel.
Claim 7
Dependent Claim
14 words
The method of claim 1 or 2 wherein the silver salt is silver nitrate.
Claim 8
Dependent Claim
23 words
The method of claim 1 or 2 wherein the reducing solution comprises a base of either aqueous potassium or sodium hydroxide and formaldehyde.
Claim 9
Dependent Claim
12 words
The method of claim 7 wherein a 5% glutaraldehyde solution is employed.
Claim 10
Dependent Claim
101 words
The method of claim 1 or 2 wherein said substance is treated with a 2.5 to 25% glutaraldehyde solution for up to 12 hours, then is washed for one to 6 hours in distilled water, is equilibrated in an aqueous silver salt solution for 10 to 120 minutes, after which said substance is subjected to a reducing solution for about 30 seconds to 5 minutes, then is immersed in a first aqueous carbonate or sulfate salt solution for about one to 15 minutes, then a second and third aqueous carbonate or sulfate salt solution for about one to 15 minutes each.
Claim 11
Dependent Claim
12 words
The method of claim 9 wherein the carbonate salt is sodium carbonate.
Claim 12
Independent Claim
131 words
A method for staining a protein, polypeptide or protein containing substance which is supported in a matrix which comprises the steps of treating said substance with a 2.5-25% glutaraldehyde solution for one to 6 hours, washing said substance one to 6 hours with distilled water, equilibrating said substance for about two hours in an aqueous silver nitrate solution containing from about 0.5 to 6 grams of silver salts per liter of water, then subjecting said substance for about 30 seconds to 5 minutes to a reducing solution comprising about 0.5 to 2 ml of 37% formalin per about 150 ml of 0.5 to 1.0 N aqueous sodium hydroxide followed by immersing said substance in 3 sequential solutions of 0.075 to 1.5% aqueous sodium carbonate for about one to 5 minutes each.
Claim 13
Independent Claim
131 words
A method for staining a protein, polypeptide or protein containing substance which is supported in a matrix which comprises the steps of treating said substance with a 2.5-25% glutaraldehyde solution for one to 6 hours, washing said substance one to 6 hours with distilled water, equilibrating said substance for about two hours in an aqueous silver nitrate solution containing from about 0.5 to 6 grams of silver salts per liter of water, then subjecting said substance for about 30 seconds to 5 minutes to a reducing solution comprising about 0.5 to 2 ml of 37% formalin per about 150 ml of 0.5 to 1.0 N aqueous sodium hydroxide followed by immersing said substance in 3 sequential solutions of 0.075 to 1.5% aqueous sodium carbonate for about one to 5 minutes each.
Claim 14
Dependent Claim
28 words
A mercantile kit comprising multiple containers separately holding a concentrated solution of glutaraldehyde, a silver salt, a base solution, an aldehyde solution and a carbonate or sulfate salt.
Claim 15
Dependent Claim
28 words
A mercantile kit comprising multiple containers separately holding a concentrated solution of glutaraldehyde, a silver salt, a base solution, an aldehyde solution and a carbonate or sulfate salt.
Claim 16
Dependent Claim
56 words
The kit of claim 12 wherein one container has therein a 60 ml 25% glutaraldehyde; one container has therein 0.4 to 1.0 grams of silver nitrate; another container has therein 9 ml of 50% sodium hydroxide; another container has therein 1.2 ml of 37% formalin; and one additional container has therein 3.4 grams of sodium carbonate.
References Cited (Backward Citations)
US4239495A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |