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US4467030A
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Determination of the thyroxine-binding index in serum
Assignee: Roche Diagnostics GmbH
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Filing: 1981-05-15
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Granted: 1984-08-21
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Expires: 2001-05-15
Abstract
Abstract A method for the determination of the thyroxine-binding index in serum, which method comprises mixing the serum sample to be determined with a defined amount of thyroxine and a defined amount of a determinable enzyme covalently bound to thyroxine, contacting the resulting solution with anti-thyroxine antibodies present in the solid phase, separating the liquid phase from the solid phase, and measuring the activity of said determinable enzyme in one of the phases as a measure of the tyroxine-binding index in said serum sample.
Inventors
- 👤 Gerd Kleinhammer
- 👤 Gerlinde Deutsch
- 👤 Hans-Ralf Linke
- 👤 Fritz Stahler
- 👤 Wolfgang Gruber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
83 words
Method for determining the thyroxine-binding index in serum comprising: (a) mixing a sample of the serum with (1) a predetermined amount of thyroxine and (2) a predetermined amount of enzyme-labeled thyroxine; (b) contacting the solution resulting from step (a) with insolubilized anti-thyroxine; (c) separating the resulting liquid phase from the resulting solid phase; (d) measuring the enzyme activity in either the liquid or the solid phase of step (c); and (e) relating the measured enzyme activity to the thyroxine-binding index in the serum.
Claim 2
Dependent Claim
83 words
Method for determining the thyroxine-binding index in serum comprising: (a) mixing a sample of the serum with (1) a predetermined amount of thyroxine and (2) a predetermined amount of enzyme-labeled thyroxine; (b) contacting the solution resulting from step (a) with insolubilized anti-thyroxine; (c) separating the resulting liquid phase from the resulting solid phase; (d) measuring the enzyme activity in either the liquid or the solid phase of step (c); and (e) relating the measured enzyme activity to the thyroxine-binding index in the serum.
Claim 3
Dependent Claim
24 words
Method as in claim 1 wherein the predetermined amount of thyroxine is in an amount of 100 to 500 ng per milliliter of serum.
Claim 4
Dependent Claim
11 words
Method as in claim 1 wherein the enzyme label is peroxidase.
Claim 5
Dependent Claim
18 words
Method as in claim 3 wherein the peroxidase-labeled thyroxine is in an amount of 1 to 10 mu/ml.
Claim 6
Dependent Claim
13 words
Method as in claim 1 wherein the anti-thyroxine is bound to a carrier.
Claim 7
Dependent Claim
16 words
Method as in claim 5 wherein the carrier is the inner wall of a test tube.
Claim 8
Dependent Claim
45 words
Test kit for determining the thyroxine-binding index in serum comprising the following separately contained components: (a) predetermined amount of thyroxine; (b) predetermined amount of enzyme-labeled thyroxine; (c) anti-thyroxine bound to a solid material; (d) buffer; and (e) reagent for determining the activity of said enzyme.
Claim 9
Dependent Claim
45 words
Test kit for determining the thyroxine-binding index in serum comprising the following separately contained components: (a) predetermined amount of thyroxine; (b) predetermined amount of enzyme-labeled thyroxine; (c) anti-thyroxine bound to a solid material; (d) buffer; and (e) reagent for determining the activity of said enzyme.
Claim 10
Dependent Claim
47 words
Test kit as in claim 7 consisting essentially of ______________________________________
thyroxine 100-400 ng/ml.
thyroxine-peroxidase 5-100 mU/ml.
barbital buffer (pH 8.6)
0.1-0.2 M
bovine serum albumin 0.2%
antithyroxine 1-0.05/ng/ml.
(calculated without carrier)
hydrogen peroxidase 0.5-5 mM/l.
2,2'-azino-di- 5-50 mM/l.
(3-ethylbenzthiazoline-
6-sulphonate)
phosphate-citrate buffer
0.1-0.2 M
(pH. 5.0)
______________________________________
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-21 | 1988-02-20 | 1988-08-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-21 | 1992-02-20 | 1992-08-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-21 | 1996-02-20 | 1996-08-21 | $7700.00 | Window Closed / Lapsed |