US4040907A
Expired / Lapsed
US
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Iodothyronine enzyme conjugates
Assignee: Syva Co
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Filing: 1975-12-29
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Granted: 1977-08-09
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Expires: 1995-12-29
Abstract
Abstract Polyiodothyronine conjugates to enzymes are provided which find use in the determination of polyiodothyronine compounds, particularly thyroxine, normally in physiological fluids, such as serum. The enzymes can provide substantial variation in activity when bound to protein receptors for polyiodothyronine as compared to the free or unbound polyiodothyronine conjugated to the enzyme. Various linking groups are provided for linking the polyiodothyronine to the enzyme.
Inventors
- 👤 Edwin F. Ullman
- 👤 Kenneth E. Rubenstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
61 words
A thyroxine conjugate to an enzyme at other than the active site of the enzyme, having on the average from about 1 to 20 of said thyroxines bonded to said enzyme, wherein said thyroxine conjugated enzyme undergoes a substantial change in the enzymatic activity of said enzyme upon binding of receptor for said thyroxine to the thyroxine conjugated to said enzyme.
Claim 2
Dependent Claim
61 words
A thyroxine conjugate to an enzyme at other than the active site of the enzyme, having on the average from about 1 to 20 of said thyroxines bonded to said enzyme, wherein said thyroxine conjugated enzyme undergoes a substantial change in the enzymatic activity of said enzyme upon binding of receptor for said thyroxine to the thyroxine conjugated to said enzyme.
Claim 3
Dependent Claim
161 words
A thyroxine enzyme conjugate of the formula ##STR4## wherein: any one of the W groups can be X or an H of any one of the W groups may be replaced by X, wherein X is a bond or linking group; X is 0 or 1, being 0 when W 1 is the linking group; A is an enzyme bonded at other than its active site; n is on the average in the range of 1 to the molecular weight by A divided by 2,000; α 1-4 are iodine; L is hydrogen or when W 2 is the linking group, lower alkyl of from 1 to 3 carbon atoms or a protective acyl group; when other than a linking group: W and W 2 are hydrogen, with the proviso that when L is lower alkyl, W is lower alkyl of from 1 to 3 carbon atoms; and W 1 is hydroxyl or lower alkoxyl of from 1 to 3 carbon atoms.
Claim 4
Dependent Claim
161 words
A thyroxine enzyme conjugate of the formula ##STR4## wherein: any one of the W groups can be X or an H of any one of the W groups may be replaced by X, wherein X is a bond or linking group; X is 0 or 1, being 0 when W 1 is the linking group; A is an enzyme bonded at other than its active site; n is on the average in the range of 1 to the molecular weight by A divided by 2,000; α 1-4 are iodine; L is hydrogen or when W 2 is the linking group, lower alkyl of from 1 to 3 carbon atoms or a protective acyl group; when other than a linking group: W and W 2 are hydrogen, with the proviso that when L is lower alkyl, W is lower alkyl of from 1 to 3 carbon atoms; and W 1 is hydroxyl or lower alkoxyl of from 1 to 3 carbon atoms.
Claim 5
Dependent Claim
18 words
A polyiodothyronine conjugate according to claim 2, wherein W is the linking group and A is an oxidoreductase.
Claim 6
Dependent Claim
19 words
A polyiodothyronine enzyme conjugate according to claim 2, wherein W is the linking group and A is a hydrolase.
Claim 7
Dependent Claim
19 words
A polyiodothyronine enzyme conjugate according to claim 2, wherein W is the linking group and A is an isomerase.
Claim 8
Dependent Claim
20 words
A polyiodothyronine enzyme conjugate according to claim 2, wherein W 2 is the linking group and A is an oxidoreductase.
Claim 9
Dependent Claim
104 words
A polyiodothyronine enzyme conjugate according to the formula ##STR5## wherein: Y is hydrogen or lower alkyl of from 1 to 3 carbon atoms; n is on the average in the range of 1 to 20; A is an enzyme bonded at other than the active site to the polyiodothyronine group in the parenthesis; W is a linking group which is a bond or aliphatic group of from 1 to 16 atoms other than hydrogen, which are carbon, oxygen, nitrogen, or sulphur, the oxygen and sulphur being present as oxy or oxo or thioanalogs thereof and the nitrogen being present as amido or tertiary amino.
Claim 10
Dependent Claim
104 words
A polyiodothyronine enzyme conjugate according to the formula ##STR5## wherein: Y is hydrogen or lower alkyl of from 1 to 3 carbon atoms; n is on the average in the range of 1 to 20; A is an enzyme bonded at other than the active site to the polyiodothyronine group in the parenthesis; W is a linking group which is a bond or aliphatic group of from 1 to 16 atoms other than hydrogen, which are carbon, oxygen, nitrogen, or sulphur, the oxygen and sulphur being present as oxy or oxo or thioanalogs thereof and the nitrogen being present as amido or tertiary amino.
Claim 11
Dependent Claim
23 words
A polyiodothyronine enzyme conjugate according to claim 7, wherein n is in the range of 2 to 12 and A is an oxidoreductase.
Claim 12
Dependent Claim
23 words
A polyiodothyronine enzyme conjugate according to claim 7, wherein m is in the range of 2 to 12 and A is an hydrolase.
Claim 13
Dependent Claim
90 words
A polyiodothyronine enzyme conjugate according to claim 7, wherein W is of the formula ##STR6## wherein: M and M 1 are chalcogen or nitrogen; m and p are either 0 or 1, the sum of m plus p being in the range of 0 to 2; R is a bond or an aliphatic group of from 1 to 8 carbon atoms and includes from 0 to 1 heteroatom of atomic number 7 to 8, oxygen being present as oxy or oxo and nitrogen being present as tertiary amino or amido.
Claim 14
Dependent Claim
31 words
A thyroxine conjugate to an enzyme at other than the active site of the enzyme, having on the average from about 1 to 20 of said thyroxine bonded to said enzyme.
Claim 15
Dependent Claim
31 words
A thyroxine conjugate to an enzyme at other than the active site of the enzyme, having on the average from about 1 to 20 of said thyroxine bonded to said enzyme.
Claim 16
Dependent Claim
28 words
A thyroxine conjugate to glucose-6-phosphate dehydrogenase having from 1 to 12 thyroxine groups bonded to said glucose-6-phosphate dehydrogenase by amide linkages or the nitrogen or thio analog thereof.
Claim 17
Dependent Claim
28 words
A thyroxine conjugate to glucose-6-phosphate dehydrogenase having from 1 to 12 thyroxine groups bonded to said glucose-6-phosphate dehydrogenase by amide linkages or the nitrogen or thio analog thereof.
Claim 18
Dependent Claim
20 words
A thyroxine conjugate according to claim 12, wherein said thyroxine is bonded through the thyroxine amino by a diglycoldioyl group.
Claim 19
Dependent Claim
28 words
A thyroxine conjugate to glucose oxidase having from 1 to 12 thyroxine groups bonded to said glucose oxidase by amido linkages or the nitrogen or thio analogs thereof.
Claim 20
Dependent Claim
28 words
A thyroxine conjugate to glucose oxidase having from 1 to 12 thyroxine groups bonded to said glucose oxidase by amido linkages or the nitrogen or thio analogs thereof.
Claim 21
Dependent Claim
26 words
A thyroxine conjugate to lysozyme having from 1 to 5 thyroxine groups bonded to said lysozyme by amide linkages or the nitrogen or thio analogs thereof.
Claim 22
Dependent Claim
26 words
A thyroxine conjugate to lysozyme having from 1 to 5 thyroxine groups bonded to said lysozyme by amide linkages or the nitrogen or thio analogs thereof.
Claim 23
Dependent Claim
27 words
A desaminothyrozine conjugate to glucose-6-dehydrogenase having from 1 to 12 desaminothyroxine groups bonded to said glucose-6-phosphate dehydrogenase by amide linkages or the nitrogen or thio analog thereof.
Claim 24
Dependent Claim
27 words
A desaminothyrozine conjugate to glucose-6-dehydrogenase having from 1 to 12 desaminothyroxine groups bonded to said glucose-6-phosphate dehydrogenase by amide linkages or the nitrogen or thio analog thereof.
References Cited (Backward Citations)
US3850752A
Cited
US3839153A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1980-08-08 | 1981-02-07 | 1981-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-08 | 1985-02-07 | 1985-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-08 | 1989-02-07 | 1989-08-09 | $7700.00 | Window Closed / Lapsed |