Patent US4459226A successfully refreshed from Google Patents.
US4459226A
Expired / Lapsed
US
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Process for recovering insulin
Assignee: Eli Lilly and Co
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Filing: 1982-02-26
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Granted: 1984-07-10
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Expires: 2002-02-26
Abstract
Abstract Insulin can be recovered for processing and re-use from an insulin-protamine complex by bringing the complex into contact with an aqueous medium having a pH of from about 2 to about 5 and containing an insulin dissociating or depolymerizing agent, a cation exchanger, and a salt at a concentration of from about 0.2 M to about 0.6 M.
Inventors
- 👤 Denise L. Grimes
- 👤 C. Stephen Hollinden
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:49
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; C07K14/625
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
83 words
A process for recovering insulin from an insulin-protamine complex, which comprises ( 1) bringing the insulin-protamine complex into contact with an aqueous medium having a pH from about 2 to about 5 and containing urea at a concentration of from about 2 M to about 8 M, a cation exchanger selected from the group consisting of SP Sephadex and CM Sephadex, and salt at a concentration of from about 0.1 M to about 0.6 M, and (2) recovering insulin having reduced protamine content.
Claim 2
Dependent Claim
83 words
A process for recovering insulin from an insulin-protamine complex, which comprises ( 1) bringing the insulin-protamine complex into contact with an aqueous medium having a pH from about 2 to about 5 and containing urea at a concentration of from about 2 M to about 8 M, a cation exchanger selected from the group consisting of SP Sephadex and CM Sephadex, and salt at a concentration of from about 0.1 M to about 0.6 M, and (2) recovering insulin having reduced protamine content.
Claim 3
Dependent Claim
27 words
A process of claim 1, in which the salt is sodium chloride and is present at a concentration of from about 0.2 M to about 0.5 M.
Claim 4
Dependent Claim
34 words
A process of claim 2, in which the insulin-protamine complex is brought into contact with urea, the cation exchanger, and the salt at a temperature of from about 4° C. to about 8° C.
Claim 5
Dependent Claim
32 words
A process of claim 3, in which the insulin dissociating or depolymerizing agent is urea present in the aqueous medium at a concentration of from about 2 M to about 8 M.
Claim 6
Dependent Claim
23 words
A process of claim 3, in which the urea is present at a concentration of from about 5 M to about 8 M.
Claim 7
Dependent Claim
13 words
A process of claim 5, in which the cation exchanger is SP Sephadex.
Claim 8
Dependent Claim
23 words
A process of claim 6, in which the salt is present at a concentration of from about 0.35 M to about 0.5 M.
Claim 9
Dependent Claim
16 words
A process of claim 7, in which the pH is from about 3.5 to about 4.5.
Claim 10
Dependent Claim
14 words
A process of claim 1, in which insulin is recovered substantially free of protamine.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-10 | 1988-01-09 | 1988-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-10 | 1992-01-09 | 1992-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-10 | 1996-01-09 | 1996-07-10 | $7700.00 | Window Closed / Lapsed |