Photometric methods for counting the particulate components of blood

Claim 1

A method for determining the white blood cell and platelet concentration in whole blood, comprising the steps of preparing a hypertonic solution, said solution being distilled water to which is added from about 1% to 9% by weight of Sodium Benzoate and from about 1% to 8% by weight of a polysaccharide selected from the group of dextran having an average molecular weight of about 200,000 to 300,000, wherein the total percentage of said sodium benzoate and polysaccharide will range from about 2% to 17% by weight, adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being from about one part of whole blood to 250 parts of said hypertonic solution to one part of whole blood to 2000 parts of said hypertonic solution, mixing thoroughly the blood bearing solution, allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate, placing a measured portion of the blood bearing solution in a transparent envelope, surrounding said transparent envelope with Nylon IV dyed to render it absorbent of radiation across the entire visible and ultraviolet spectrum, exposing said solution to radiation that will cause the solution to produce a visible spectrum response which is quantitatively related to the white blood cell and platelet concentration and measuring said visible spectrum response, and wherein red blood cells are removed from the whole blood prior to measuring said visible spectrum response.

Claim 2

A method for determining the white blood cell and platelet concentration in whole blood, comprising the steps of preparing a hypertonic solution, said solution being distilled water to which is added from about 1% to 9% by weight of Sodium Benzoate and from about 1% to 8% by weight of a polysaccharide selected from the group of dextran having an average molecular weight of about 200,000 to 300,000, wherein the total percentage of said sodium benzoate and polysaccharide will range from about 2% to 17% by weight, adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being from about one part of whole blood to 250 parts of said hypertonic solution to one part of whole blood to 2000 parts of said hypertonic solution, mixing thoroughly the blood bearing solution, allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate, placing a measured portion of the blood bearing solution in a transparent envelope, surrounding said transparent envelope with Nylon IV dyed to render it absorbent of radiation across the entire visible and ultraviolet spectrum, exposing said solution to radiation that will cause the solution to produce a visible spectrum response which is quantitatively related to the white blood cell and platelet concentration and measuring said visible spectrum response, and wherein red blood cells are removed from the whole blood prior to measuring said visible spectrum response.

Claim 6

A method for determining the white blood cell and platelet concentration in whole blood comprising the steps of preparing a hypertonic solution, said solution being distilled water to which is added about 3% by weight of sodium benzoate, 6% by weight of dextran having an average molecular weight of about 200,000 to 300,00, adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being about one part of whole blood to five hundred parts of hypertonic solution, mixing thoroughly the blood bearing solution, allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate, placing a measured portion of the blood bearing solution in a transparent envelope, surrounding said transparent envelope with Nylon IV dyed to render it absorbent of radiation across the entire visible and ultraviolet spectrum, exposing said solution to radiation that will cause the solution to produce a visible spectrum response which is quantitatively related to the white blood cell and platelet concentration and measuring said visible spectrum response, wherein red blood cells are removed from the whole blood prior to measuring said visible spectrum response.

Claim 7

A method for determining the white blood cell and platelet concentration in whole blood comprising the steps of preparing a hypertonic solution, said solution being distilled water to which is added about 3% by weight of sodium benzoate, 6% by weight of dextran having an average molecular weight of about 200,000 to 300,00, adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being about one part of whole blood to five hundred parts of hypertonic solution, mixing thoroughly the blood bearing solution, allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate, placing a measured portion of the blood bearing solution in a transparent envelope, surrounding said transparent envelope with Nylon IV dyed to render it absorbent of radiation across the entire visible and ultraviolet spectrum, exposing said solution to radiation that will cause the solution to produce a visible spectrum response which is quantitatively related to the white blood cell and platelet concentration and measuring said visible spectrum response, wherein red blood cells are removed from the whole blood prior to measuring said visible spectrum response.

Claim 8

A method for determining the degree of agglutination of red blood cells comprising the steps of: (a) preparing a hypertonic solution, said solution being distilled water to which is added from about 1% to 9% by weight of sodium benzoate and from about 1% to 8% by weight of a polysaccharide, selected from the group of dextran having an average molecular weight of 200,000 to 300,000, wherein the total percentage of said sodium benzoate and polysaccharide will be about 2% to about 17% by weight; (b) adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being from about one part whole blood to about 250 to 2000 parts of said hypertonic solution; (c) mixing thoroughly the blood bearing solution; (d) allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate; (e) placing a measured portion of the blood bearing solution in a transparent envelope; (f) exposing said solution to radiation so as to cause the blood bearing solution to produce a visible spectrum response which is quantitatively related to the red blood cell concentration; (g) monitoring said visible spectrum response related to the red blood cell concentration; (h) relating the absorbence change of red blood cell concentration to the degree of agglutination of said red blood cells.

Claim 9

A method for determining the degree of agglutination of red blood cells comprising the steps of: (a) preparing a hypertonic solution, said solution being distilled water to which is added from about 1% to 9% by weight of sodium benzoate and from about 1% to 8% by weight of a polysaccharide, selected from the group of dextran having an average molecular weight of 200,000 to 300,000, wherein the total percentage of said sodium benzoate and polysaccharide will be about 2% to about 17% by weight; (b) adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being from about one part whole blood to about 250 to 2000 parts of said hypertonic solution; (c) mixing thoroughly the blood bearing solution; (d) allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate; (e) placing a measured portion of the blood bearing solution in a transparent envelope; (f) exposing said solution to radiation so as to cause the blood bearing solution to produce a visible spectrum response which is quantitatively related to the red blood cell concentration; (g) monitoring said visible spectrum response related to the red blood cell concentration; (h) relating the absorbence change of red blood cell concentration to the degree of agglutination of said red blood cells.

Claim 10

The method as stated in claim, 6 wherein the radiation that will cause the solution to produce a visible spectrum response has a wavelength selected from the group of radiation wavelengths of 540 nm and 578 nm.

Claim 11

A method for determining the degree of agglutination of red blood cells comprising the steps of: (a) preparing a hypertonic solution, said solution being distilled water to which is added from about 1% to 9% by weight of sodium benzoate and from about 1% to 8% by weight of a polysaccharide, selected from the group of dextran having an average molecular weight of 200,000 to 300,000, wherein the total percentage of said sodium benzoate and polysaccharide will be about 2% to about 17% by weight; (b) adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being from about one part whole blood to about 250 to 2000 parts of said hypertonic solution; (c) mixing thoroughly the blood bearing solution; (d) allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate; (e) placing a measured portion of the blood bearing solution in a transparent envelope; (f) surrounding said transparent envelope with Nylon IV dyed to render it absorbent of radiation across the entire visible and ultraviolet spectrum; (g) exposing said solution to radiation so as to cause the blood bearing solution to produce a visible spectrum response which is quantitatively related to the concentration of the red blood cell concentration; (h) monitoring said visible spectrum response related to the red blood cell concentration; (i) relating the absorbence change of red blood cell concentration to the degree of agglutination of said red blood cells.

Claim 12

A method for determining the degree of agglutination of red blood cells comprising the steps of: (a) preparing a hypertonic solution, said solution being distilled water to which is added from about 1% to 9% by weight of sodium benzoate and from about 1% to 8% by weight of a polysaccharide, selected from the group of dextran having an average molecular weight of 200,000 to 300,000, wherein the total percentage of said sodium benzoate and polysaccharide will be about 2% to about 17% by weight; (b) adding whole blood to said hypertonic solution to form a blood bearing solution, said whole blood additions to said hypertonic solution being from about one part whole blood to about 250 to 2000 parts of said hypertonic solution; (c) mixing thoroughly the blood bearing solution; (d) allowing the solution to stand for a minimum of one minute to allow the cells to fully crenate; (e) placing a measured portion of the blood bearing solution in a transparent envelope; (f) surrounding said transparent envelope with Nylon IV dyed to render it absorbent of radiation across the entire visible and ultraviolet spectrum; (g) exposing said solution to radiation so as to cause the blood bearing solution to produce a visible spectrum response which is quantitatively related to the concentration of the red blood cell concentration; (h) monitoring said visible spectrum response related to the red blood cell concentration; (i) relating the absorbence change of red blood cell concentration to the degree of agglutination of said red blood cells.