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US4452887A
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Integral multi-layered element containing glucose oxidase for determining glucose
Assignee: Fujifilm Holdings Corp
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Filing: 1982-06-17
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Granted: 1984-06-05
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Expires: 2002-06-17
Abstract
Abstract In a dry-type multilayer liquid analysis material for glucose determination composed of a transparent water-impermeable support having formed thereon in sequence at least a reagent layer containing a reactive component forming a detectable material by the action of hydrogen peroxide, a non-porous light-shielding layer, and a porous spreading layer, the coloring efficiency for colorimetry using the liquid analysis material is improved by incorporating a glucose oxidase enzyme in at least one layer disposed above the reagent layer.
Inventors
- 👤 Masao Kitajima
- 👤 Fuminori Arai
- 👤 Harumi Katsuyama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 05:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
63 words
An integral multi-layered element for determining glucose in a liquid sample comprising a water-impermeable light-transmissive support having formed thereon, in sequence, a reagent layer containing a reactive component forming a detectable material by the action of hydrogen peroxide, a non-porous light-shielding layer and a porous spreading layer, wherein a glucose oxidase is incorporated in at least one layer disposed above said reagent layer.
Claim 2
Dependent Claim
63 words
An integral multi-layered element for determining glucose in a liquid sample comprising a water-impermeable light-transmissive support having formed thereon, in sequence, a reagent layer containing a reactive component forming a detectable material by the action of hydrogen peroxide, a non-porous light-shielding layer and a porous spreading layer, wherein a glucose oxidase is incorporated in at least one layer disposed above said reagent layer.
Claim 3
Dependent Claim
31 words
The element as claimed in claim 1 wherein a timing layer is formed between said reagent layer and said non-porous light-shielding layer or said layer in which glucose oxidase is incorporated.
Claim 4
Dependent Claim
25 words
The element as claimed in claim 1 or 2 wherein an adhesive layer is formed between said non-porous light-shielding layer and said porous spreading layer.
Claim 5
Dependent Claim
45 words
The element as claimed in any of claims 1 to 3 wherein said layer disposed above said reagent layer is selected from the non-porous light-shielding layer, the porous spreading layer, the timing layer, and adhesive layer, and the layer in which glucose oxidase is incorporated.
References Cited (Backward Citations)
US3992158A
Cited
US4042335A
Cited
US4144306A
Cited
US4166093A
Cited
US4255384A
Cited
US4258001A
Cited
US4353984A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-05 | 1987-12-05 | 1988-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-05 | 1991-12-05 | 1992-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-05 | 1995-12-05 | 1996-06-05 | $7700.00 | Window Closed / Lapsed |