Patent US3234666A successfully refreshed from Google Patents.
US4450231A
Expired / Lapsed
US
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Immunoassay for determination of immune complexes with polymer-coated plastic base
Assignee: BIOSTAR MEDICAL PRODUCTS Inc A Co CORP
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Filing: 1982-03-31
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Granted: 1984-05-22
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Expires: 2002-03-31
Abstract
Abstract An immunoassay of a specimen of a serum or the like to determine immune complexes, includes producing on a plastic base a layer of a non-proteinaceous, non-ionic polymer which will adhere to the plastic base and has the capability of absorbing immune complexes of the specimen, placing a specimen on the layer and treating the layer to produce an indication of the amount of immune complexes. The polymer may be polyethylene glycol, dextran, polyvinyl chloride, a polymeric polyol or an adduct of polyethylene glycol. Washing with conventional solutions, addition of an antihuman IgG coupled with an enzyme and addition of a substrate reactive therewith, are similar to the ELISA test, with color measurement as by spectrophotometer. Or, the addition of anti IgG-I 125 and measurement by a scintillation counter may be used. The ethylene glycol may range in molecular weight from 2,000 to 20,000, although 6,000 to 8,000 is preferred. A product for use in such an assay is a plate having wells or a test tube formed of plastic, polystyrene and polyvinyl chloride being preferred, with a layer of such non-proteinaceous, non-ionic layer on the plate wells or the cavity of the test tube. This product, if it is not to be used immediately, should be provided with a layer or film protecting the polymer layer from the air.
Inventors
- 👤 Adil N. Ozkan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/564; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
112 words
In a method of performing an immunoassay of a fluid specimen to determine immune complexes therein, the steps comprising: treating a solid phase, plastic base having means for receiving the fluid specimen to be assayed, to produce a layer of a non-proteinaceous non-ionic polymer on said means, said polymer adhering to said plastic base and having the capability of absorbing immune complexes which may be present in such specimen; placing the fluid specimen to be assayed on said receiving means; permitting said polymer layer to absorb immune complexes present in said specimen; and treating said layer in a manner to produce an indication of the amount of immune complexes in said layer.
Claim 2
Dependent Claim
112 words
In a method of performing an immunoassay of a fluid specimen to determine immune complexes therein, the steps comprising: treating a solid phase, plastic base having means for receiving the fluid specimen to be assayed, to produce a layer of a non-proteinaceous non-ionic polymer on said means, said polymer adhering to said plastic base and having the capability of absorbing immune complexes which may be present in such specimen; placing the fluid specimen to be assayed on said receiving means; permitting said polymer layer to absorb immune complexes present in said specimen; and treating said layer in a manner to produce an indication of the amount of immune complexes in said layer.
Claim 3
Dependent Claim
24 words
In a method as defined in claim 1, wherein: said polymer is selected from the group consisting of polyethylene glycol, dextran and polyvinyl chloride.
Claim 4
Dependent Claim
25 words
In a method as defined in claim 1, wherein: said non-ionic polymer is polyethylene glycol having a molecular weight from about 2000 to about 20,000.
Claim 5
Dependent Claim
15 words
In a method as defined in claim 1, wherein: said polymer is a polymeric polyol.
Claim 6
Dependent Claim
40 words
In a method as defined in claim 1, wherein: said indication treatment produces a change in color, generally proportional to the amount of immune complexes absorbed by said layer; and measuring the color so produced by visual or spectrophotometric means.
Claim 7
Dependent Claim
146 words
In a method as defined in claim 5, which includes: washing said base a plurality of times with a buffer solution following production of said polymer layer thereon; washing said plate, after absorption of immune complexes of said specimen by said polymer layer, a plurality of times with a buffer solution; adding an anti-human IgG coupled with an enzyme to said layer and permitting said layer to stand for a predetermined time; washing said layer a plurality of times with a buffer solution after said layer is permitted to stand for said predetermined time; adding a substrate reactive with said anti-human IgG coupled with an enzyme to produce a color change proportional to the amount of said anti-human IgG coupled with an enzyme which has reacted with immune complexes present in said layer; and measuring the color change in said layer by means of a spectrophotometer.
Claim 8
Dependent Claim
40 words
In a method as defined in claim 1, wherein: said indication treatment involves a radioactive agent which produces a radiation intensity generally proportional to the amount of immune complex absorbed by said layer; and measuring the amount of radiation present.
Claim 9
Dependent Claim
43 words
In a method as defined in claim 1, which includes: treating a second fluid specimen, essentially identical to said first specimen, with a virus or bacteria of a suspected condition; and performing an immunoassay on said second specimen simultaneously with said first specimen.
Claim 10
Dependent Claim
104 words
In a method of performing an immunoassay of a fluid specimen to detect immune complexes therein, the steps comprising: placing the fluid specimen on a receiving means of a solid phase, plastic base having at least one receiving means, with said receiving means having a layer of a non-proteinaceous, non-ionic polymer adhering thereto and having the capability of absorbing immune complexes from such specimens; placing the fluid specimen to be assayed on said receiving means; permitting said polymer layer to absorb such immune complexes; and treating said layer in a manner to provide an indication of the amount of immune complexes in said layer.
Claim 11
Dependent Claim
104 words
In a method of performing an immunoassay of a fluid specimen to detect immune complexes therein, the steps comprising: placing the fluid specimen on a receiving means of a solid phase, plastic base having at least one receiving means, with said receiving means having a layer of a non-proteinaceous, non-ionic polymer adhering thereto and having the capability of absorbing immune complexes from such specimens; placing the fluid specimen to be assayed on said receiving means; permitting said polymer layer to absorb such immune complexes; and treating said layer in a manner to provide an indication of the amount of immune complexes in said layer.
Claim 12
Dependent Claim
24 words
In a method as defined in claim 9, wherein: said polymer is selected from the group consisting of polyethylene glycol, dextran and polyvinyl chloride.
Claim 13
Dependent Claim
25 words
In a method as defined in clim 9, wherein: said non-ionic polymer is polyethylene glycol having a molecular weight from about 2000 to about 20,000.
Claim 14
Dependent Claim
25 words
In a method as defined in clim 9, wherein: said non-ionic polymer is polyethylene glycol having a molecular weight from about 2000 to about 20,000.
Claim 15
Dependent Claim
15 words
In a method as defined in claim 9, wherein: said polymer is a polymeric polyol.
Claim 16
Dependent Claim
35 words
In a method as defined in claim 9, wherein: said indication treatment produces a change in color, generally proportional to the amount of immune complexes absorbed by said layer; and measuring the color so produced.
Claim 17
Dependent Claim
146 words
In a method as defined in claim 13, which includes; washing said base a plurality of times with a buffer solution following production of said polymer layer thereon; washing said plate, after absorption of immune complexes of said specimen by said polymer layer, a plurality of times with a buffer solution; adding an anti-human IgG coupled with an enzyme to said layer and permitting said layer to stand for a predetermined time; washing said layer a plurality of times with a buffer solution after said layer is permitted to stand for said predetermined time; adding a substrate reactive with said anti-human IgG coupled with an enzyme to produce a color change proportional to the amount of said anti-human IgG coupled with an enzyme which has reacted with immune complexes present in said layer; and measuring the color change in said layer by means of a spectrophotometer.
Claim 18
Dependent Claim
40 words
In a method as defined in claim 9, wherein: said indication treatment involves a radioactive agent which produces a radiation intensity generally proportional to the amount of immune complex absorbed by said layer; and measuring the amount of radiation present.
Claim 19
Dependent Claim
43 words
In a method as defined in claim 9, which includes: treating a second fluid specimen, essentially identical to said first specimen, with a virus or bacteria of a suspected condition; and performing an immunoassay on said second specimen simultaneously with said first specimen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-22 | 1987-11-21 | 1988-05-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-22 | 1991-11-21 | 1992-05-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-22 | 1995-11-21 | 1996-05-22 | $7700.00 | Window Closed / Lapsed |