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US4443546A
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Process and composition for propagating mammalian cells
Assignee: Beth Israel Deaconess Medical Center
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Filing: 1982-02-01
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Granted: 1984-04-17
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Expires: 2002-02-01
Abstract
Abstract A serum-free medium for use in propagating cells. A process involving growing normal mammalian cells in vitro in a serum-free medium. The process includes providing the cells to be grown with various hormones as well as required nutrients in a serum-free environment.
Inventors
- 👤 Michael B. Stemerman
- 👤 Robert Weinstein
- 👤 Thomas Maciag
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:39
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; C12N5/0037; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/20; C12N2500/24; C12N2500/25; C12N2500/30; C12N2500/32; C12N2500/38; C12N2500/70; C12N2500/80; C12N2500/84; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/115; C12N2501/135; C12N2501/30; C12N2501/38; C12N2501/39; C12N2501/70
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
44 words
A serum-free cell growth medium comprising: EGF: 1.0 ng/ml-10 μg/ml, Cohn fraction IV (modified): 1.0 μg/ml-100 μg/ml, Thrombin: 100 ng/ml-10 μg/ml, Insulin: 10 ng/ml-10 μg/ml, Transferrin: 10 ng/ml-10 μg/ml, ECGF: 500 ng/ml-150 μg/ml, Hydrocortisone: 5×10 -7 M-5×10 -4 M, Nutrient source: Medium 199 (balance).
Claim 2
Dependent Claim
44 words
A serum-free cell growth medium comprising: EGF: 1.0 ng/ml-10 μg/ml, Cohn fraction IV (modified): 1.0 μg/ml-100 μg/ml, Thrombin: 100 ng/ml-10 μg/ml, Insulin: 10 ng/ml-10 μg/ml, Transferrin: 10 ng/ml-10 μg/ml, ECGF: 500 ng/ml-150 μg/ml, Hydrocortisone: 5×10 -7 M-5×10 -4 M, Nutrient source: Medium 199 (balance).
Claim 3
Dependent Claim
43 words
A serum-free cell growth medium comprising: EGF: 1.0 ng/ml-10 μg/ml, FGF: 1.0 ng/ml-10 μg/ml, Cohn fraction IV (modified): 1.0 μg/ml-100 μg/ml, Thrombin: 100 ng/ml-10 μg/ml, Insulin: 10 ng/ml-10 μg/ml, Transferrin: 10 ng/ml-10 μg/ml, Hydrocortisone: 5×10 -7 M-5×10 -4 M, Nutrient source: DME (balance).
Claim 4
Dependent Claim
43 words
A serum-free cell growth medium comprising: EGF: 1.0 ng/ml-10 μg/ml, FGF: 1.0 ng/ml-10 μg/ml, Cohn fraction IV (modified): 1.0 μg/ml-100 μg/ml, Thrombin: 100 ng/ml-10 μg/ml, Insulin: 10 ng/ml-10 μg/ml, Transferrin: 10 ng/ml-10 μg/ml, Hydrocortisone: 5×10 -7 M-5×10 -4 M, Nutrient source: DME (balance).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-04-17 | 1987-10-17 | 1988-04-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-04-17 | 1991-10-17 | 1992-04-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-04-17 | 1995-10-17 | 1996-04-17 | $7700.00 | Window Closed / Lapsed |