US4124448A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the large scale production of human growth hormone by serial secondary suspension culture
Assignee: Source Technology Inc
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Filing: 1976-04-09
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Granted: 1978-11-07
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Expires: 1996-04-09
Abstract
Abstract A new system of serially culturing human anterior pituitary gland cells in a new nutrient medium to produce large amounts of human growth hormone. Since only human growth hormone can be used to treat growth deficiencies in man, there is a great demand for the hormone, which is in relative short supply since only 2 to 3 mgs. of the human growth hormone can be extracted from one human pituitary gland obtained at autopsy. Using the new system of culture and new nutrient media, the human pituitary cells can be grown in vitro to produce in approximately three weeks, more than 20 times the amount of extractable growth hormone than that which was originally present in the original tissue now used for extracting the 2 to 3 mgs. of the hormone.
Inventors
- 👤 Mandayam J. Narasimhan
- 👤 John A. Anderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 20:32
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
47 words
A nutrient culture medium composition adapted to the production of growth hormone from pituitary cells by serial subculturing, said composition consisting essentially of effective amounts of an amino acid-rich nutrient medium supplemented by the addition of minor amounts of liver extract, insulin, and anti-biotic and anti-fungal agents.
Claim 2
Dependent Claim
47 words
A nutrient culture medium composition adapted to the production of growth hormone from pituitary cells by serial subculturing, said composition consisting essentially of effective amounts of an amino acid-rich nutrient medium supplemented by the addition of minor amounts of liver extract, insulin, and anti-biotic and anti-fungal agents.
Claim 3
Dependent Claim
21 words
A composition according to claim 1 further characterized in that the amino acid-rich medium is Medium 199-1X with Earle's modified salts.
Claim 4
Dependent Claim
35 words
A composition according to claim 2 further characterized in that said medium composition includes about 5 to 20 ml of liver extract and about 6 to 20 I.U. of insulin per liter of Medium 199-1X.
Claim 5
Dependent Claim
58 words
A composition according to claim 1 further characterized in that said medium composition consists essentially of Medium 199-1X with Earle's modified salts supplemented by about 10 ml injectable liver extract containing B vitamins, about 10 I.U. crystalline insulin, about 500,000 units crystalline sodium penicillin G, about 500 mg streptomycin sulfate and about 50,000 units of nystatin per liter.
Claim 6
Dependent Claim
110 words
A process for the production of growth hormone which comprises: (A) preparing a nutrient amino acid-rich medium composition according to claim 1 in an open aeration incubation vessel, (B) dispersing cells of the anterior pituitary in said medium, (C) initially maintaining said cells under aerated cell growth conditions for several days, (D) sub-culturing the resulting initial culture and reconstituting the original volume with additional medium, (E) maintaining the sub-cultures under aerated cell growth conditions for an additional several days, (F) serially sub-culturing every several days until the desired optimum growth level is reached, and (G) separating the media from the cells and extracting the growth hormone from the sub-culture cells.
Claim 7
Dependent Claim
21 words
A process according to claim 5 further characterized in that said pituitary is human and said hormone is human growth hormone.
Claim 8
Dependent Claim
21 words
A process according to claim 5 further characterized in that the amino acid-rich medium is Medium 199-1X with Earle's modified salts.
Claim 9
Dependent Claim
35 words
A process according to claim 7 further characterized in that said medium composition includes about 5 to 20 ml of liver extract and about 6 to 20 I.U. of insulin per liter of Medium 199-1X.
Claim 10
Dependent Claim
58 words
A process according to claim 6 further characterized in that said medium composition consists essentially of Medium 199-1X with Earle's modified salts supplemented by about 10 ml injectable liver extract containing B vitamins, about 10 I.U. crystalline insulin, about 500,000 units crystalline sodium penicillin G, about 500 mg streptomycin sulfate and about 50,000 units of nystatin per liter.
Claim 11
Dependent Claim
19 words
A process according to claim 6 further characterized in that said initial culture is incubated for about 8 days.
Claim 12
Dependent Claim
20 words
A process according to claim 6 further characterized in that said sub-cultures are incubated for about 4 to 8 days.
Claim 13
Dependent Claim
24 words
A process according to claim 6 further characterized in that said initial culture is sub-cultured at least 4 times to obtain optimal growth level.
Claim 14
Dependent Claim
25 words
A process according to claim 6 further characterized in that said cultures are aerated by a positive flow of sterile air without added carbon dioxide.
References Cited (Backward Citations)
US3073746A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-06 | 1982-05-08 | 1982-11-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-06 | 1986-05-08 | 1986-11-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-06 | 1990-05-08 | 1990-11-07 | $7700.00 | Window Closed / Lapsed |