Patent US4432895A successfully refreshed from Google Patents.
US4432895A
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Monomeric interferons
Assignee: Hoffmann La Roche Inc
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Filing: 1982-11-24
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Granted: 1984-02-21
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Expires: 2002-11-24
Abstract
Abstract A method for producing monomeric interferons from oligomeric interferons, for preventing formation of oligomeric interferon from monomeric interferon, and for increasing the yield of monomeric interferon upon purification of interferon is described. The method employs a redox reagent to treat the interferon sample.
Inventors
- 👤 Stanley J. Tarnowski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:03
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/56; C07K1/00; C07K1/107; C07K1/113; C07K1/1133; Y; Y10; Y10S; Y10S530/00; Y10S530/808
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
57 words
A method for producing biologically active interferon in which essentially all of the interferon is in monomeric form which comprises treating a sample of purified or partially purified interferon with an effective amount of a redox reagent to convert oligomeric interferon to biologically active monomeric interferon and to prevent the formation of oligomeric interferon from monomeric interferon.
Claim 2
Independent Claim
57 words
A method for producing biologically active interferon in which essentially all of the interferon is in monomeric form which comprises treating a sample of purified or partially purified interferon with an effective amount of a redox reagent to convert oligomeric interferon to biologically active monomeric interferon and to prevent the formation of oligomeric interferon from monomeric interferon.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein the interferon is recombinant human leukocyte interferon.
Claim 4
Dependent Claim
29 words
A method according to claim 1 wherein the redox reagent is selected from a group consisting of reduced glutathione and oxidized glutathione, cysteine and cystine, and cysteamine and cystamine.
Claim 5
Dependent Claim
14 words
A method according to claim 1 wherein the redox reagent is a buffered solution.
Claim 6
Dependent Claim
14 words
A method according to claim 4 wherein the buffered solution is about pH 7.5.
Claim 7
Independent Claim
42 words
A method for producing biologically active monomeric interferon in a sample containing purified or partially purified oligomeric interferon, the method comprising treating the sample with an effective amount of a redox reagent to convert the oligomeric interferon to biologically active monomeric interferon.
Claim 8
Independent Claim
42 words
A method for producing biologically active monomeric interferon in a sample containing purified or partially purified oligomeric interferon, the method comprising treating the sample with an effective amount of a redox reagent to convert the oligomeric interferon to biologically active monomeric interferon.
Claim 9
Dependent Claim
14 words
A method according to claim 6 wherein the interferon is recombinant human leukocyte interferon.
Claim 10
Dependent Claim
29 words
A method according to claim 6 wherein the redox reagent is selected from a group consisting of reduced glutathione and oxidized glutathione, cysteine and cystine, and cysteamine and cystamine.
Claim 11
Dependent Claim
14 words
A method according to claim 6 wherein the redox reagent is a buffered solution.
Claim 12
Dependent Claim
14 words
A method according to claim 9 wherein the buffered solution is about pH 7.5.
Claim 13
Independent Claim
101 words
A method for preparing interferon in monomeric form from a sample of partially purified cell-free extracts containing interferon comprising: (a) passing the sample through an affinity chromatographic column which contains anti-interferon antibody which binds the interferon; (b) washing the column with a solution which will remove non-antibody binding material from the column but which will not significantly affect the antibody bound interferon; (c) eluting the column with a solution which will provide active fractions containing the interferon; and (d) treating the active fractions with an effective amount of a redox agent to provide thereby active fractions containing interferon in monomeric form.
Claim 14
Independent Claim
101 words
A method for preparing interferon in monomeric form from a sample of partially purified cell-free extracts containing interferon comprising: (a) passing the sample through an affinity chromatographic column which contains anti-interferon antibody which binds the interferon; (b) washing the column with a solution which will remove non-antibody binding material from the column but which will not significantly affect the antibody bound interferon; (c) eluting the column with a solution which will provide active fractions containing the interferon; and (d) treating the active fractions with an effective amount of a redox agent to provide thereby active fractions containing interferon in monomeric form.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-20 | 1987-08-22 | 1988-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-20 | 1991-08-22 | 1992-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-20 | 1995-08-22 | 1996-02-21 | $7700.00 | Window Closed / Lapsed |