Patent US4429040A successfully refreshed from Google Patents.
US4429040A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and reagent for the detection of fibrin monomer
Assignee: Roche Diagnostics GmbH
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Filing: 1981-04-21
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Granted: 1984-01-31
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Expires: 2001-04-21
Abstract
Abstract The present invention provides a process for the detection or determination of fibrin monomer in biological fluids, wherein the sample fluid is incubated with hydrophobic latex and the agglutination determined. The present invention also provides a reagent for the detection or determination of fibrin or fibrin monomer in biological fluids, wherein it contains hydrophobic latex.
Inventors
- 👤 Udo Becker
- 👤 Peter Roeschlau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/75; Y; Y10; Y10S; Y10S435/00; Y10S435/962
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
45 words
Method for the determination of fibrin monomer in a biological fluid sample comprising incubating said fluid sample with a reagent consisting essentially of hydrophobic latex under conditions suitable for agglutination, and determining the resulting agglutination as a measure of fibrin monomer content in the sample.
Claim 2
Dependent Claim
45 words
Method for the determination of fibrin monomer in a biological fluid sample comprising incubating said fluid sample with a reagent consisting essentially of hydrophobic latex under conditions suitable for agglutination, and determining the resulting agglutination as a measure of fibrin monomer content in the sample.
Claim 3
Dependent Claim
20 words
Method as claimed in claim 1, wherein said hydrophobic latex has an average particle diameter of from 0.05 to 1.5μ.
Claim 4
Dependent Claim
26 words
Method as claimed in claim 1, wherein the incubation is carried out at a temperature of from 30° to 40° C. for 5 to 15 minutes.
Claim 5
Dependent Claim
24 words
Method as claimed in claim 1, wherein the incubation is carried out in a buffered solution at a pH of from 7.0 to 9.0.
Claim 6
Dependent Claim
31 words
Method as claimed in claim 1, wherein, the sample, after incubation with the latex, is applied to a dark-colored test plate and the agglutination ascertained, to result in a qualitative determination.
Claim 7
Dependent Claim
32 words
Method as claimed in claim 1, wherein serial dilutions of the sample fluid are prepared and compared with a standard with a known content of FS, to result in a quantitative determination.
Claim 8
Dependent Claim
25 words
Method as claimed in claim 1, wherein the change of turbidity brought about by the agglutination is measured optically, to result in a quantitative determination.
Claim 9
Dependent Claim
30 words
Method as claimed in claim 1, wherein, the fibrinogen present in the sample fluid is first converted into FS with the use of thrombin or of a snake venom enzyme.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-30 | 1987-08-01 | 1988-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-30 | 1991-08-01 | 1992-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-30 | 1995-08-01 | 1996-01-31 | $7700.00 | Window Closed / Lapsed |