US4427580A
Expired / Lapsed
US
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Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts
Assignee: Cornell Research Foundation Inc
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Filing: 1982-09-01
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Granted: 1984-01-24
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Expires: 2002-09-01
Abstract
Abstract The present invention comprises a process for treating nucleoprotein complexes with a chaotropic salt in order to dissociate the nucleic acids from the nucleo-protein complexes, and then isoelectrically precipitating a nucleic acid diminished protein containing fraction from a nucleic acid enriched supernatant.
Inventors
- 👤 John E. Kinsella
- 👤 Damodaran Srinivasan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 12:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6806; A; A23; A23J; A23J1/00; A23J1/008; A23J1/18; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/821; Y10S530/825
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
59 words
A method of reducing nucleic acid in microbially derived protein which comprises: (a) disrupting microbial cells to provide a mixture comprising protein and nucleic acid, (b) mixing the mixture of protein and nucleic acid with an effective nucleoprotein complex dissociating amount of a chaotropic salt, and (c) reducing the pH to isoelectrically precipitate a nucleic acid diminished protein concentrate.
Claim 2
Independent Claim
59 words
A method of reducing nucleic acid in microbially derived protein which comprises: (a) disrupting microbial cells to provide a mixture comprising protein and nucleic acid, (b) mixing the mixture of protein and nucleic acid with an effective nucleoprotein complex dissociating amount of a chaotropic salt, and (c) reducing the pH to isoelectrically precipitate a nucleic acid diminished protein concentrate.
Claim 3
Dependent Claim
23 words
A method as in claim 1 wherein the protein-nucleic acid mixture is derived from Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, or Saccharomyces carlsbergensis.
Claim 4
Independent Claim
33 words
A method as in claims 1 or 2 wherein the chaotropic salt is selected from the group consisting of the sodium or potassium salt of perchlorate, thiocyanate, trichloroacetate, nitrate, iodide, bromide or urea.
Claim 5
Independent Claim
25 words
A method of claims 1, 2 or 3 wherein the isoelectric precipitation of the protein concentrate is conducted at a pH of 4.0 to 4.8.
Claim 6
Dependent Claim
56 words
In a method of forming a nucleic acid diminished protein concentrate from a mixture of protein and nucleic acid, the steps comprising: (a) mixing the mixture of protein and nucleic acid with an effective nucleoprotein complex dissociating amount of a chaotropic salt, and (b) reducing the pH to isoelectrically precipitate a nucleic acid diminished protein concentrate.
Claim 7
Dependent Claim
56 words
In a method of forming a nucleic acid diminished protein concentrate from a mixture of protein and nucleic acid, the steps comprising: (a) mixing the mixture of protein and nucleic acid with an effective nucleoprotein complex dissociating amount of a chaotropic salt, and (b) reducing the pH to isoelectrically precipitate a nucleic acid diminished protein concentrate.
Claim 8
Dependent Claim
62 words
In a method for preparing soluble ribonucleic acids containing reduced amounts of proteinaceous materials, the steps comprising: (a) mixing the mixture of protein and nucleic acid with an effective nucleoprotein complex dissociating amount of a chaotropic salt, (b) reducing the pH to isoelectrically precipitate a nucleic acid diminished protein concentrate, and (c) separating and recovering the ribonucleic acids from the protein concentrate.
Claim 9
Dependent Claim
62 words
In a method for preparing soluble ribonucleic acids containing reduced amounts of proteinaceous materials, the steps comprising: (a) mixing the mixture of protein and nucleic acid with an effective nucleoprotein complex dissociating amount of a chaotropic salt, (b) reducing the pH to isoelectrically precipitate a nucleic acid diminished protein concentrate, and (c) separating and recovering the ribonucleic acids from the protein concentrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-23 | 1987-07-25 | 1988-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-23 | 1991-07-25 | 1992-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-23 | 1995-07-25 | 1996-01-24 | $7700.00 | Window Closed / Lapsed |