US3983008A
Expired / Lapsed
US
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Method of extracting useful components from microbial cells
Assignee: Idemitsu Kosan Co Ltd
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Filing: 1975-05-20
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Granted: 1976-09-28
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Expires: 1995-05-20
Abstract
Abstract Method of extracting a useful component from microbial cells by disrupting microbial cells. Microbial cells are disrupted in one or more stages in a high-pressure multi-stage homogenizer and said disrupted cells are contacted with solvent in one or more remaining stages of the homogenizer to extract a useful component.
Inventors
- 👤 Tatsuo Shinozaki
- 👤 Nobuyuki Kikkawa
- 👤 Fumiaki Fujitani
- 👤 Kenjiro Tanaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 16:54
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/06; C12N1/066; C12M; C12M47/00; C12M47/06; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/823; Y10S530/824; Y10S530/825
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
62 words
A method of extracting a component from microbial cells containing the same, which comprises disrupting microbial cells in a first stage in a high-pressure multi-stage homogenizer in the absence of a solvent for extracting said component from the cells, and contacting said disrupted cells with a solvent in a directly succeeding stage of said homogenizer to extract said component from the cells.
Claim 2
Independent Claim
62 words
A method of extracting a component from microbial cells containing the same, which comprises disrupting microbial cells in a first stage in a high-pressure multi-stage homogenizer in the absence of a solvent for extracting said component from the cells, and contacting said disrupted cells with a solvent in a directly succeeding stage of said homogenizer to extract said component from the cells.
Claim 3
Dependent Claim
36 words
The method according to claim 1, wherein said homogenizer is a high-pressure, two-stage homogenizer wherein the disrupting step is carried out in the first stage and the extracting step is carried out in the second stage.
Claim 4
Dependent Claim
43 words
The method according to claim 1, wherein said disrupting step is carried out at a pressure difference of from 100 to 1500 kg/cm 2 and the extracting step is carried out at a pressure difference of from 0.1 to 300 kg/cm 2 .
Claim 5
Dependent Claim
44 words
The method according to claim 2, wherein said disrupting step is carried out at a pressure difference of from 100 to 1500 kg/cm 2 , and the extracting step is carried out at a pressure difference of from 0.1 to 300 kg/cm 2 .
Claim 6
Dependent Claim
28 words
The method according to claim 1, wherein microbial cells are fed to said homogenizer as an aqueous suspension having a cell concentration of from 1 to 25% w/v.
Claim 7
Dependent Claim
27 words
The method according to claim 2, wherein microbial cells are fed to said homogenizer as an aqueous suspension having a cell concentration of 1 to 25% w/v.
Claim 8
Dependent Claim
16 words
The method according to claim 1, wherein said microbial cells are yeast cells or bacterium cells.
Claim 9
Dependent Claim
16 words
The method according to claim 2, wherein said microbial cells are yeast cells or bacterium cells.
Claim 10
Dependent Claim
15 words
The method of claim 1, wherein said first stage comprises a plurality of disruption stages.
References Cited (Backward Citations)
US2928614A
Cited
US3375174A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-09-28 | 1980-03-29 | 1980-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-09-28 | 1984-03-29 | 1984-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-09-28 | 1988-03-29 | 1988-09-28 | $7700.00 | Window Closed / Lapsed |