US4330464A
Expired / Lapsed
US
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Isolation of microbial protein with reduced nucleic acid content
Assignee: George Weston Ltd
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Filing: 1981-07-09
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Granted: 1982-05-18
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Expires: 2001-07-09
Abstract
Abstract The present invention relates to a process for the isolation of protein from microbial cells. The microbial cells are subjected to a treatment to disrupt the cell walls thereby producing a cellular debris fraction and a fraction containing protein and nucleic acid. The two fractions are separated and the fraction containing the protein and nucleic acid is treated with a chelating agent at a pH in the range of 5.5 to 7.0. The resulting solution is passed through an anion exchange column which selectively adsorbs the nucleic acid. The protein is recovered from the resultant eluate.
Inventors
- 👤 George R. Lawford
- 👤 Peter N. Lewis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 01:03
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/18; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/821
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
91 words
A process for the isolation of protein from microbial cells in which the microbial cells are subjected to a treatment to disrupt the cell walls thereby producing a cellular debris fraction and a fraction containing protein and nucleic acid, the two fractions are separated, the fraction containing the protein and nucleic acid is treated with a chelating agent at a pH in the range of from 5.5 to 7.0, the resultant solution is passed over an anion exchange resin which selectively adsorbs the nucleic acid and the resultant eluate is collected.
Claim 2
Dependent Claim
91 words
A process for the isolation of protein from microbial cells in which the microbial cells are subjected to a treatment to disrupt the cell walls thereby producing a cellular debris fraction and a fraction containing protein and nucleic acid, the two fractions are separated, the fraction containing the protein and nucleic acid is treated with a chelating agent at a pH in the range of from 5.5 to 7.0, the resultant solution is passed over an anion exchange resin which selectively adsorbs the nucleic acid and the resultant eluate is collected.
Claim 3
Dependent Claim
19 words
A process as claimed in claim 1 in which the chelating agent is citric acid or a salt thereof.
Claim 4
Dependent Claim
19 words
A process as claimed in claim 1 in which the chelating agent is ethylenediaminetetraacetic acid or a salt thereof.
Claim 5
Dependent Claim
21 words
A process as claimed in claim 1, claim 2 or claim 3 in which the cells are derived from Canadida utilis.
Claim 6
Dependent Claim
21 words
A process as claimed in claim 1, claim 2 or claim 3 in which the cells are derived from Saccharomyces cerevisiae.
Claim 7
Dependent Claim
21 words
A process as claimed in claim 1, claim 2 or claim 3 in which the cells are derived from Zymomonas mobilis.
Claim 8
Dependent Claim
21 words
A process as claimed in claim 1, claim 2 or claim 3 in which the cells are derived from Saccharomyces carlsbergensis.
Claim 9
Dependent Claim
20 words
A process as claimed in claim 1 in which the pH is in the range of from 6 to 6.5.
Claim 10
Dependent Claim
18 words
A process as claimed in claim 1, claim 2 or claim 3 in which the eluate is dried.
Claim 11
Dependent Claim
21 words
A process as claimed in claim 1, claim 2 or claim 3 in which the eluate is desalted and then dried.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-17 | 1985-11-16 | 1986-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-17 | 1989-11-16 | 1990-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-17 | 1993-11-16 | 1994-05-18 | $7700.00 | Window Closed / Lapsed |