Patent US4424201A successfully refreshed from Google Patents.
US4424201A
Expired / Lapsed
US
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Employment of a mereyanine dye for the detection of malignant leukocytic cells
Assignee: Rockefeller University
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Filing: 1978-11-28
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Granted: 1984-01-03
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Expires: 1998-11-28
Abstract
Abstract A method for the detection of malignant leukocytic cells which comprises: contacting a fluid containing malignant leukocytic cells with a merocyanine dye of the formula: ##STR1## wherein n=1-5; X=0 or S; R 1 and R 2 are the same or different C 2 -C 5 alkyl groups: wherein said dye is incorporated into said cells; separating the cells from the incubation mixture; and detecting said dyed cells. Fluorescence or radioactivity detection methods are preferred detecting means.
Inventors
- 👤 Jay E. Valinsky
- 👤 Edward Reich
- 👤 Thomas G. Easton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 07:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/811; Y10S436/813
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
70 words
A method for the detection of malignant leukocytic cells which comprises contacting a fluid solution containing malignant leukocytic cells with a merocyanine dye of the formula ##STR5## wherein n=1-5; X=0 or S and R 1 and R 2 are the same or different C 2 -C 5 alkyl groups; wherein said dye is incorporated into said cells; separating the cells from the incubation mixture and then detecting said dyed cells.
Claim 2
Independent Claim
70 words
A method for the detection of malignant leukocytic cells which comprises contacting a fluid solution containing malignant leukocytic cells with a merocyanine dye of the formula ##STR5## wherein n=1-5; X=0 or S and R 1 and R 2 are the same or different C 2 -C 5 alkyl groups; wherein said dye is incorporated into said cells; separating the cells from the incubation mixture and then detecting said dyed cells.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein n=3; X=S; R 1 =R 2 =--C 4 H 9 .
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein said detection is by fluorescence.
Claim 5
Dependent Claim
16 words
The method of claim 3 wherein said detection of fluorescence is carried out by darkfield-fluorescence microscopy.
Claim 6
Dependent Claim
17 words
The method of claim 3 wherein said detection of fluorescence is carried out by flow microfluorimetric methods.
Claim 7
Dependent Claim
21 words
The method of claim 3 wherein said fluorescence is produced by excitation radiation of a wavelength in the range 400-700 nm.
Claim 8
Dependent Claim
14 words
The method of claim 1 wherein said contact is effected in a saline solution.
Claim 9
Dependent Claim
12 words
The method of claim 7 wherein said saline solution contains autologous plasma.
Claim 10
Dependent Claim
24 words
The method of claim 1 wherein the concentration of said cells in said fluid is in the range 10 5 to 5×10 7 cells/ml.
Claim 11
Dependent Claim
22 words
The method of claim 1 wherein the concentration of said dye in said solution is in the range 0.1 to 200 μg/ml.
Claim 12
Dependent Claim
15 words
The method of claim 1 wherein the temperature of contacting is 0° to 40° C.
Claim 13
Dependent Claim
11 words
The method of claim 1 wherein said merocyanine dye is radioactive.
Claim 14
Dependent Claim
15 words
The method of claim 12 wherein said merocyanine dye is that wherein X= 35 S.
Claim 15
Dependent Claim
25 words
The method of claim 11 wherein said detection is done by determining radioactivity uptake of dye by said leukocytic cells present in said fluid solution.
Claim 16
Dependent Claim
21 words
The method of claim 11 wherein the specific activity of said radioactive dye in said solution is 0.1 to 100 Ci/mole.
Claim 17
Dependent Claim
17 words
The method of claim 1 wherein said contacting is carried out in the presence of ambient illumination.
Claim 18
Dependent Claim
26 words
The method of claim 1, wherein the incubation of said merocyanine dye with said fluid solution is carried out in the presence of trypan blue dye.
Claim 19
Dependent Claim
36 words
The method of claim 1 wherein said malignant leukocytic cells are leukemic cells which are selected from the group consisting of chronic and acute leukemias of the lymphocytic, myelocytic, myelocyticmonocytic, monocytic, nonlymphoblastic, hystocytic and lymphoma types.
Claim 20
Independent Claim
82 words
A method of detecting solid tumors which comprises contacting a fluid solution containing leukocytic cells derived from the blood of patients having, suspected of having or having had a solid malignant tumor, with a merocyanine dye of the formula ##STR6## wherein n=1-5; X=0 or S, and R 1 and R 2 are the same or different C 2 -C 5 alkyl groups; wherein said dye is incorporated into said cells; separating the cells from the incubation mixture and detecting said dyed cells.
Claim 21
Independent Claim
82 words
A method of detecting solid tumors which comprises contacting a fluid solution containing leukocytic cells derived from the blood of patients having, suspected of having or having had a solid malignant tumor, with a merocyanine dye of the formula ##STR6## wherein n=1-5; X=0 or S, and R 1 and R 2 are the same or different C 2 -C 5 alkyl groups; wherein said dye is incorporated into said cells; separating the cells from the incubation mixture and detecting said dyed cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-02 | 1987-07-04 | 1988-01-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-02 | 1991-07-04 | 1992-01-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-02 | 1995-07-04 | 1996-01-03 | $7700.00 | Window Closed / Lapsed |