US3673410A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of examination of cell samples using a radioactively tagged dye
Assignee: Individual
•
Filing: 1970-06-09
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Granted: 1972-06-27
•
Expires: 1990-06-09
Abstract
Abstract A sample of cells from a body under investigation is treated with a radioactively tagged substance which is determined to be selectively absorbed by one or more predetermined cell types sought to be identified either as disease producing cells or abnormal cells of the body and distinguished from other cell types. The radioactively tagged substance is aborbed in different amounts by some cells and rejected by other cells. After treatment and washing, the level of radioactivity of the cell sample is sensed in order to determine from the degree of absorption of the radioactively tagged substance information about the presence of absence of disease. The technique may be applied to mass screening for disease of a particular type or types by collecting and identifying similar samples from many subjects and using a predetermined common standard of radiation level to eliminate those samples which are clearly free from disease.
Inventors
- 👤 John H Waite
- 👤 John H Nodine
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 07:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/02; C12Q1/04; C12Q1/16; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/60
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
20 words
The method of claim 1 in which the dye employed has affinity for diseased cells or microorganisms within the sample.
Claim 2
Dependent Claim
34 words
The method of claim 1 in which the sample is one of tissue or cells and the dye employed is selected for its affinity to cells of a selected type sought to be identified.
Claim 3
Dependent Claim
33 words
The method of claim 3 in which the cells sought to be identified are cancer cells and the dye selected has greater affinity for cancerous than for normal cells of the same type.
Claim 4
Dependent Claim
11 words
The method of claim 4 in which cervix cells are examined.
Claim 5
Dependent Claim
13 words
The method of claim 4 in which blood cells are examined for lukemia.
Claim 6
Dependent Claim
12 words
The method of claim 3 in which the lung tissue is examined.
Claim 7
Dependent Claim
11 words
The method of claim 3 in which prostate tissue is examined.
Claim 8
Dependent Claim
33 words
The method of claim 1 in which the sample is one potentially containing microorganisms of a predetermined type and the dye employed is selected for its affinity to micro-organism of that predetermined type.
Claim 9
Dependent Claim
37 words
The method of claim 9 in which the microorganisms sought to be identified are parasites of selected type and the dye selected has greater affinity for such parasiTes than other substances which may occur in the sample.
Claim 10
Dependent Claim
11 words
The method of claim 10 in which malaria parasites are examined.
Claim 11
Dependent Claim
11 words
The method of claim 10 in which trapanosomiasis parasites are examined.
Claim 12
Dependent Claim
37 words
The method of claim 9 in which the microorganisms sought to be identified are bacteria of selected type and the dye selected has greater affinity for such bacteria than other substances which may occur in the sample.
Claim 13
Dependent Claim
37 words
The method of claim 9 in which the microorganisms sought to be identified are fungi of selected type and the dye selected has greater affinity for such fungi than other substances which may occur in the sample.
Claim 14
Dependent Claim
12 words
The method of claim 13 in which samples are examined for pneumonicocus.
Claim 15
Dependent Claim
12 words
The method of claim 13 in which samples are examined for tuberculosis.
Claim 16
Dependent Claim
12 words
The method of claim 13 in which samples are examined for vaginitis.
Claim 17
Dependent Claim
13 words
The method of claim 13 in which samples are examined for intestinal diseases.
Claim 18
Dependent Claim
13 words
The method of claim 1 employing 3H hematoxylin as the radioactively tagged dye.
Claim 19
Dependent Claim
14 words
The method of claim 1 employing 3H basic fuchsin as the radioactively tagged dye.
Claim 20
Dependent Claim
14 words
The method of claim 1 employing 14C methylene blue as the radioactively tagged dye.
Claim 21
Dependent Claim
24 words
The method of claim 1 in which the radioactively tagged substance employed also colors the cells of predetermined type and is rejected by others.
Claim 22
Dependent Claim
16 words
The method of claim 1 in which non-colorimetric radioactive tagging of the sample is of significance.
Claim 23
Dependent Claim
41 words
The method of claim 1 in which the fixed sample contains cells of the predetermined type of a size too small to show by colorimetric staining techniques but which will absorb the radioactively tagged substance in sufficient amounts for positive identification.
Claim 24
Dependent Claim
33 words
The method of claim 1 in which the information content in the fixed sample itself is digitalized directly through the radioactive emission so that it can be directly used as a computer input.
Claim 25
Independent Claim
158 words
The method of mass screening of a plurality of subjects for disease of a particular type, comprising collecting and identifying similar samples of tissue, cells or microorganisms from a plurality of subjects, and preparing the samples as fixed samples for staining, staining each sample with a radioactively tagged dye which is determined to be selectively absorbed by samples of the type under investigation so that the affinity of the dye to the cells, tissues or microorganisms is utilized to bind the radioisotope of the tagged dye to the fixed tissues, cells or microorganisms, appropriately washing the samples, sensing the level of radioactivity of each sample, from said levels of radioactivity sensed, eliminating those samples which are clearly free from disease by comparing the level of radioactivity of each sample with a predetermined common standard of radiation level, and further processing those samples not shown to be clearly free from disease to determine the presence or absence of disease.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-06-27 | 1975-12-27 | 1976-06-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-06-27 | 1979-12-27 | 1980-06-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-06-27 | 1983-12-27 | 1984-06-27 | $7700.00 | Window Closed / Lapsed |