Patent US4416812A successfully refreshed from Google Patents.
US4416812A
Expired / Lapsed
US
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Method of preparing tissue thromboplastin
Assignee: Roche Diagnostics GmbH
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Filing: 1982-12-01
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Granted: 1983-11-22
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Expires: 2002-12-01
Abstract
Abstract A tissue thromboplastin preparation which is sensitive to coagulation factor VII, is prepared from acetone dry powder mammalian tissue, by extraction with a salt solution containing 1 to 20 mmol/1 of calcium ions and, if desired, a surface active agent, after which the extract maybe dried.
Inventors
- 👤 Udo Becker
- 👤 Eugen Schaich
- 👤 Manfred Weigert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:30
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; C07; C07K; C07K14/00; C07K14/435; C07K14/745; A; A61; A61K; A61K38/00; G; G01; G01N; G01N2333/00; G01N2333/435; G01N2333/745; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/839
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
37 words
A method of producing a tissue thromboplastin preparation which is sensitive to coagulation factor VII comprising extracting an acetone dry powder from mammalian tissue with an aqueous salt solution of 1 to 20 mmol/l of calcium ions.
Claim 2
Independent Claim
37 words
A method of producing a tissue thromboplastin preparation which is sensitive to coagulation factor VII comprising extracting an acetone dry powder from mammalian tissue with an aqueous salt solution of 1 to 20 mmol/l of calcium ions.
Claim 3
Dependent Claim
17 words
The method of claim 1, wherein said salt solution contains 5 to 15 mmol/l of calcium ions.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the calcium ions are supplied by a calcium salt of a water-soluble carboxylic acid.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein said carboxylic acid is formic or acetic acid.
Claim 6
Dependent Claim
22 words
The method of claim 1 further comprising washing the acetone dry powder with a pH 6.5 to 8 buffer solution before extracting.
Claim 7
Dependent Claim
16 words
The method of claim 1 further comprising adding a surface active agent to the extraction solution.
Claim 8
Dependent Claim
14 words
The method of claim 6 wherein 0.01 to 0.5% surface active agent is added.
Claim 9
Dependent Claim
10 words
The method of claim 1 further comprising drying the extract.
Claim 10
Dependent Claim
40 words
A reagent for the control of blood coagulating ability, comprising a factor VII sensitive tissue thromboplastin preparation obtained by extracting an acetone dry powder from mammalian tissue with an aqueous salt solution of 1 to 20 mmol/l of calcium ions.
Claim 11
Dependent Claim
40 words
A reagent for the control of blood coagulating ability, comprising a factor VII sensitive tissue thromboplastin preparation obtained by extracting an acetone dry powder from mammalian tissue with an aqueous salt solution of 1 to 20 mmol/l of calcium ions.
Claim 12
Dependent Claim
26 words
The reagent of claim 9 further including a predetermined amount of factor VII insensitive thromboplastin preparation to establish a predetermined factor VII sensitivity for the reagent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |