Patent US4411991A successfully refreshed from Google Patents.
US4411991A
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Process for fermentative production of amino acids
Assignee: Kanegafuchi Chemical Industry Co Ltd
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Filing: 1981-09-17
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Granted: 1983-10-25
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Expires: 2001-09-17
Abstract
Abstract A process is disclosed in which an amino acid-producing microorganism having an ability to assimilate lactic acid is aerobically cultivated in the presence of at least one lactic acid microorganism in an aqueous nutrient medium containing at least one carbohydrate which is assimilable by the lactic acid microorganism but nonassimilable or weakly assimilable by the amino acid-producing microorganism as the main carbon source and an accumulated amino acid is recovered from the culture broth. An industrially advantageous production of an amino acid has become feasible by utilizing inexpensive carbon sources or those organic substances in agricultural or livestock wastes that have heretofore not been effectively utilized.
Inventors
- 👤 Kan Hirakawa
- 👤 Ryoji Takakuma
- 👤 Koji Nomura
- 👤 Masami Katoh
- 👤 Kiyoshi Watanabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 20:25
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/56; C12P13/00; C12P13/04; C12P39/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843; Y10S435/853; Y10S435/859; Y10S435/862; Y10S435/872; Y10S435/885
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
110 words
In a process for producing an amino acid by mixed-cultivating microorganisms in an aqueous nutrient medium containing one or more carbohydrates, accumulating an amino acid in the resultant culture broth, and recovering the amino acid from the culture broth, the improvement comprising aerobically mixed-cultivating an amino acid-producing microorganism having an ability of assimilating lactic acid, but having no or a weak ability of assimilating at least one carbohydrate in the nutrient medium, and at least one lactic acid-producing microorganism having an ability of assimilating the carbohydrate which is nonassimilable or weakly assimilable by said amino acid-producing microorganism, whereby an amino acid is produced from the carbohydrate in one step operation.
Claim 2
Dependent Claim
110 words
In a process for producing an amino acid by mixed-cultivating microorganisms in an aqueous nutrient medium containing one or more carbohydrates, accumulating an amino acid in the resultant culture broth, and recovering the amino acid from the culture broth, the improvement comprising aerobically mixed-cultivating an amino acid-producing microorganism having an ability of assimilating lactic acid, but having no or a weak ability of assimilating at least one carbohydrate in the nutrient medium, and at least one lactic acid-producing microorganism having an ability of assimilating the carbohydrate which is nonassimilable or weakly assimilable by said amino acid-producing microorganism, whereby an amino acid is produced from the carbohydrate in one step operation.
Claim 3
Dependent Claim
37 words
The process according to claim 1 wherein the amino acid is a member selected from the group consisting of L-lysine, L-valine, L-threonine, L-glutamic acid, L-isoleucine, L-leucine, DL-alanine, L-phenylalanine, L-tyrosine, L-tryptophane, L-arginine, L-histidine, L-serine, L-glycine, and L-aspartic acid.
Claim 4
Dependent Claim
41 words
The process according to claim 1 wherein the amino acid-producing microorganism is a member belonging to a genus selected from the group consisting of Alkaligenus, Acinetobacter, Arthrobacter, Bacillus, Brevibacterium, Corynebacterium, Flavobacterium, Micrococcus, Microbacterium, Nocardia, Proteus, Serratia, Candida, Saccharomyces, Sporoboromyces, and Schizosaccharomyces.
Claim 5
Dependent Claim
90 words
The process according to claim 3 wherein the amino acid-producing microorganism is a member selected from the group consisting of the species Alkaligenes faecalis, Alkaligenes marshallii, Acinetobacter sp-38-15, Acinetobacter calcoaceticus, Arthrobacter paraffineus, Arthrobacter citreus, Arthrobacter sp-18, Bacillus circulans, Bacillu megatherium, Bacillus subtilis, Brevibacterium ammoniagenes, Brevibacterium acetylicum, Brevibacterium thiogenitalis, Brevibacterium flavum, Brevibacterium lactofermentum, Corynebacterium hydrocarboclastum, Corynebacterium acetoacidophilum, Corynebacterium glutamicum, Flavobacterium lutescens, Micrococcus luteus, Micrococcus roseus, Microbacterium ammoniafilm, Nocardia alkanoglutinosa, Nocardia erythropolis, Nocardia lyena, Proteus morganii, Serratia marcescens, Serratia plymuthicum, Saccharomyces cerevisiae, Saccharomyces oviformis, Candida humicola, Candida lypolitica, Sporoboromyces roseus, Schizosaccharomyces pombe.
Claim 6
Dependent Claim
32 words
The process according to claim 1 wherein the lactic acid microorganism is a member belonging to a genus selected from the group consisting of Lactobacillus, Streptococcus, Leuconostoc, Pediococcus, Tetracoccus, Bacillus, and Rhizopus.
Claim 7
Dependent Claim
61 words
The process according to claim 5 wherein the lactic acid microorganism is a member selected from the group consisting of the species Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus japonicus, Lactobacillus thermophilus, Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus pentosus, Streptococcus cremoris, Streptococcus thermophilus, Streptococcus lactis, Leuconostoc dextranicum, Pediococcus acidilactici, Pediococcus pentosaceus, Pediococcus soyae, Tetracoccus soyae, Bacillus coagulans, Bacillus laevolactici, and Rhizopus oryzae.
Claim 8
Dependent Claim
26 words
The process according to claim 1, claim 5, or claim 6 wherein the lactic acid microorganism is living cells immobilized in or on a supporting material.
Claim 9
Dependent Claim
27 words
The process according to claim 1, claim 5, or claim 6 wherein the lactic acid microorganism is a mutant strain resistant to antibiotics or amino acid analogues.
Claim 10
Dependent Claim
41 words
The process according to claim 1 wherein the carbohydrate is a member selected from the group consisting of glucose, fructose, sucrose, molasses, starch hydrolysates, cellulose hydrolysates, hydrol, lactose, lactose hydrolysates, fruit juice, casein whey, cheese whey, soybean whey, dextrin, and starch.
Claim 11
Dependent Claim
52 words
The process according to claim 1 wehrein the mixed-cultivation is carried out by inoculating a seed culture obtained by cultivating the amino acid-producing microorganism in an aqueous nutrient medium containing lactic acid, its salt, or a culture broth of the lactic acid microorganism, into the aqueous nutrient medium for amino acid production.
Claim 12
Dependent Claim
48 words
The process according to claim 1 wherein the mixed cultivation is carried out by inoculating the amino acid-producing microorganism into the aqueous nutrient medium for amino acid production containing lactic acid followed by precultivating the microorganism before inoculation of the lactic acid microorganism into the aqueous nutrient medium.
Claim 13
Dependent Claim
48 words
The process according to claim 1 wherein the mixed-cultivation is carried out by inoculating a seed culture obtained by aerobically cultivating both the amino acid-producing microorganism and the lactic acid microorganism in an aqueous nutrient medium containing the carbohydrate, into the aqueous nutrient medium for amino acid production.
Claim 14
Dependent Claim
40 words
The process according to claim 1 or claim 12 wherein the mixed-cultivation is carried out by maintaining the concentration of lactic acid accumulated in the culture medium during the cultivation, in the range of not more than 1.5% by weight/volume.
Claim 15
Dependent Claim
50 words
The process according to claim 13 wherein the control of the concentration of lactic acid is maintained in the range of not more than 1.5% by regulating the cultivation temperature, the pH of the culture medium, the rate of oxygen supply to the culture medium, or a combination of these.
Claim 16
Dependent Claim
17 words
The process according to claim 1 wherein the cultivation is carried out at a pH of 4.5˜7.5.
Claim 17
Dependent Claim
18 words
The process according to claim 1 wherein the cultivation is carried out at a temperature of 25°˜55° C.
Claim 18
Dependent Claim
22 words
The process according to claim 1 wherein the carbohydrate is starch or dextrine and amylase is added to the aqueous nutrient medium.
Claim 19
Dependent Claim
31 words
The process according to claim 1 wherein the lactic acid microorganism is one producing an antimicrobial substance and the amino acid-producing microorganism is a mutant strain resistant to the antimicrobial substance.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-24 | 1987-04-25 | 1987-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-24 | 1991-04-25 | 1991-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-24 | 1995-04-25 | 1995-10-25 | $7700.00 | Window Closed / Lapsed |