US3655510A
Expired / Lapsed
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Process for preparing amino acids from hydrocarbons
Assignee: KH Neochem Co Ltd
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Filing: 1968-06-14
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Granted: 1972-04-11
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Expires: 1988-06-14
Abstract
Abstract A process for producing an amino acid, such as L-glutamic acid, L-lysine, L-ornithine, L-valine and L-homoserine, by fermentation from hydrocarbons as the main carbon source. The process is conducted by culturing a mixture of a microorganism capable of assimilating hydrocarbons and a microorganism capable of producing amino acids under aerobic conditions in an aqueous nutrient medium containing at least one hydrocarbon. Large yields of amino acids may be obtained by conducting a mixed culture of, for example, Micrococcus glutamicus with a hydrocarbonassimilating microorganism such as Arthrobacter paraffineus or Brevibacterium ketoglutamicum.
Inventors
- 👤 Katsunobu Tanaka
- 👤 Kazuo Kimura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 08:10
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
27 words
The process of claim 1, wherein the hydrocarbon-assimilating microorganism belongs to a genus selected from the group consisting of Pseudomonas, Mycobacterium, Achromobacter, Corynebacterium, Arthrobacter, Brevibacterium and Micrococcus.
Claim 2
Dependent Claim
13 words
The process of claim 1, wherein the amino acid-producing microorganism is Micrococcus glutamicus.
Claim 3
Dependent Claim
23 words
The process of claim 3, wherein said amino acid is selected from the group consisting of L-glutamic acid, L-lysine, L-ornithine, L-valine and L-homoserine.
Claim 4
Dependent Claim
24 words
The process of claim 1, wherein the hydrocarbon used as the main carbon source is an N-paraffin having from 9 to 18 carbon atoms.
Claim 5
Dependent Claim
43 words
The process of claim 1, wherein seed cultures of both microorganisms are added at the same time to said nutrient medium. 7 The process of claim 1, wherein seed cultures of the microorganisms employed are added to the nutrient medium at different times.
Claim 6
Dependent Claim
15 words
The process of claim 1, wherein the amino acid-producing microorganism belongs to the genus Micrococcus.
Claim 7
Dependent Claim
91 words
A process for producing an amino acid by fermentation from hydrocarbons which comprises culturing a mixture of (1) Micrococcus glutamicus and (2) a microorganism capable of assimilating hydrocarbons and belonging to a genus selected from the group consisting of Pseudomonas, Mycobacterium, Achromobacter Corynebacterium, Arthrobacter, Brevibacterium and Micrococcus, microorganism (1) and microorganism (2) being different microorganisms, under aerobic conditions in an aqueous nutrient medium containing at least one hydrocarbon as the main carbon source, accumulating the amino acid in the resultant culture liquor, and recovering said amino acid from said culture liquor.
Claim 8
Dependent Claim
23 words
The process of group 9, wherein said amino acid is selected from the group consisting of L-glutamic acid, L-lysine, L-ornithine, L-valine and L-homoserine.
Claim 9
Dependent Claim
24 words
The process of claim 9, wherein the hydrocarbon used as the main carbon source is an N-paraffin having from nine to 18 carbon atoms.
Claim 10
Dependent Claim
27 words
The process of claim 9, wherein culturing is carried out at a temperature of about 20* to 40* cC.nd at a pH of about 4.0 to 9.0.
Claim 11
Dependent Claim
96 words
A process for producing an amino acid by Fermentation from hydrocarbons which comprises culturing a mixture of (1) Micrococcus glutamicus ATCC 13,058 or Micrococcus glutamicus ATCC 13,287 and (2)Arthrobacter paraffineus ATCC 15,591, Brevibacterium Ketoglutamicum Atcc 15,588 or Pseudomonas aeruginosa ATCC 15,246 under aerobic conditions in an aqueous nutrient medium containing at least one hydrocarbon as the main carbon source at a temperature of about 20* to 40* C. and at a pH of about 4.0 to 9.0, accumulating the amino acid in the resultant culture liquor, and recovering said amino acid from said culture liquor. 18
Claim 12
Dependent Claim
23 words
The process of claim 13, wherein said amino acid is selected from the group consisting of L-glutamic acid, L-lysine, L-ornithine, L-valine and L-homoserine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-04-11 | 1975-10-11 | 1976-04-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-04-11 | 1979-10-11 | 1980-04-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-04-11 | 1983-10-11 | 1984-04-11 | $7700.00 | Window Closed / Lapsed |