Method of preparing amino acids by fermentation

Claim 1

A METHOD OF PRODUCING AN AMINO ACID WHICH COMPRISES CULTURING A MICROORGANISM UNDER AEROBIC CONDITIONS ON A MEDIUM CONTAINING A HYDROCARBON AS A PRINCIPAL SOURCE OF ASSIMILABLE CARBON, AND A SOURCE OF ASSIMILABLE NITROGEN, AND RECOVERING SAID AMINO ACID FROM SAID MEDIUM, SAID MICROORGANISM BEING CAPABLE OF GROWING IN ANY ONE OF FOUR MEDIA, THE FIRST MEDIUM CONSISTING OF 10 GRAMS KEROSENE, 2 GRAMS AMMONIUM CHLORIDE, ONE GRAM DISODIUM HYDROGEN PHOSPHATE,DODEKAHYDRATE, 1.5 GRAMS POTASSIUM DIHYDROGEN PHOSPHATE, 0.5 GRAM MAGNESIUM SULFATE HEPTAHYDRATE, 2 GRAMS SODIUM CHLORIDE, AND SUFFICIENT DISTILLED WATER TO MAKE ONE LITER; THE SECOND MEDIUM CONSISTING OF 10 GRAMS PETROLEUM, 2.5 GRANS AMMONIUM NITRATE, ONE GRAM DISODIUM HYDROGEN PHOSPHATE DODEKAHYDRATE, 1.5 GRAMS POTASSIUM DIHYDROGEN PHOSPHATE, 1.5 GRAMS MAGNESIUM SULFATE HEPTAHYDRATE, 3.2 GRAM MANGANESE CHLORIDE, TRACES EACH OF CALCIUM CHLORIDE, FERROUS SULFATE, AND MOLYBDENUM SULFATE, FIVE GRAMS CALCIUM CARBONATE, AND SUFFICIENT DISTILLED WATER TO MAKE ONE LITER; THE THIRD MEDIUM CONSISTING OF ONE GRAM AMMONIUM CHLORIDE, 0.5 GRAM POTASSIUM DIHYDROGEN PHOSPHATE, 0.2 GRAM MAGNESIUM SULFATE, A TRACE OF FERRIC CHLORIDE, SUFFICIENTE WATER TO MAKE ONE LITER, AND BEING SATURATED WITH A MIXTURE OF EQUAL PARTS OF AIR AND A COMPOUND SELECTED FROM THE GROUP CONSISTING OF METHANE AND PROPANE; AND THE FOURTH MEDIUM CONSISTING OF ANY ONE OF THE FIRST, SECOND AND THIRD MEDIUM, AND OF AT LEAST ONE GROWTH PROMOTING AGENT SELECTED FROM THE GROUP CONSISTING OF YEAST EXTRACT, CORN STEEP LIQUOR, MEAT EXTRACT, PEPTONE, PROTEIN HYDROLYZATE, AND PASTES AND EXTRACTS PREPARED FROM CRUSHED ANIMAL AND VEGETAL TISSUE.

Claim 2

A METHOD OF PRODUCING AN AMINO ACID WHICH COMPRISES CULTURING A MICROORGANISM UNDER AEROBIC CONDITIONS ON A MEDIUM CONTAINING A HYDROCARBON AS A PRINCIPAL SOURCE OF ASSIMILABLE CARBON, AND A SOURCE OF ASSIMILABLE NITROGEN, AND RECOVERING SAID AMINO ACID FROM SAID MEDIUM, SAID MICROORGANISM BEING CAPABLE OF GROWING IN ANY ONE OF FOUR MEDIA, THE FIRST MEDIUM CONSISTING OF 10 GRAMS KEROSENE, 2 GRAMS AMMONIUM CHLORIDE, ONE GRAM DISODIUM HYDROGEN PHOSPHATE,DODEKAHYDRATE, 1.5 GRAMS POTASSIUM DIHYDROGEN PHOSPHATE, 0.5 GRAM MAGNESIUM SULFATE HEPTAHYDRATE, 2 GRAMS SODIUM CHLORIDE, AND SUFFICIENT DISTILLED WATER TO MAKE ONE LITER; THE SECOND MEDIUM CONSISTING OF 10 GRAMS PETROLEUM, 2.5 GRANS AMMONIUM NITRATE, ONE GRAM DISODIUM HYDROGEN PHOSPHATE DODEKAHYDRATE, 1.5 GRAMS POTASSIUM DIHYDROGEN PHOSPHATE, 1.5 GRAMS MAGNESIUM SULFATE HEPTAHYDRATE, 3.2 GRAM MANGANESE CHLORIDE, TRACES EACH OF CALCIUM CHLORIDE, FERROUS SULFATE, AND MOLYBDENUM SULFATE, FIVE GRAMS CALCIUM CARBONATE, AND SUFFICIENT DISTILLED WATER TO MAKE ONE LITER; THE THIRD MEDIUM CONSISTING OF ONE GRAM AMMONIUM CHLORIDE, 0.5 GRAM POTASSIUM DIHYDROGEN PHOSPHATE, 0.2 GRAM MAGNESIUM SULFATE, A TRACE OF FERRIC CHLORIDE, SUFFICIENTE WATER TO MAKE ONE LITER, AND BEING SATURATED WITH A MIXTURE OF EQUAL PARTS OF AIR AND A COMPOUND SELECTED FROM THE GROUP CONSISTING OF METHANE AND PROPANE; AND THE FOURTH MEDIUM CONSISTING OF ANY ONE OF THE FIRST, SECOND AND THIRD MEDIUM, AND OF AT LEAST ONE GROWTH PROMOTING AGENT SELECTED FROM THE GROUP CONSISTING OF YEAST EXTRACT, CORN STEEP LIQUOR, MEAT EXTRACT, PEPTONE, PROTEIN HYDROLYZATE, AND PASTES AND EXTRACTS PREPARED FROM CRUSHED ANIMAL AND VEGETAL TISSUE.