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Patent US4409140A successfully refreshed from Google Patents.
US4409140A
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Substrates and method for determining enzymes
Assignee: Individual
•
Filing: 1981-06-15
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Granted: 1983-10-11
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Expires: 2001-06-15
Abstract
Abstract A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.
Inventors
- 👤 Robert E. Smith
- 👤 Eugene R. Bissell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:46
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C07; C07D; C07D311/00; C07D311/02; C07D311/04; C07D311/06; C07D311/08; C07D311/16; C07K; C07K5/00; C07K5/04; C07K5/06; C07K5/06191; C07K5/08; C07K5/0827; C07K5/10; C07K5/1027; C12Q2337/00; C12Q2337/20; C12Q2337/24
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
58 words
A proteolytic enzyme substrate of the formula ##STR6## wherein R is an amino acid, a peptide, an amino acid which has been reacted with a blocking group, or a peptide, the terminal amino acid of which has been reacted with a blocking group; and R is releaseable therefrom by enzymatic hydrolysis to form a measureable amount of 7-amino-4-trifluoromethylcoumarin.
Claim 2
Dependent Claim
58 words
A proteolytic enzyme substrate of the formula ##STR6## wherein R is an amino acid, a peptide, an amino acid which has been reacted with a blocking group, or a peptide, the terminal amino acid of which has been reacted with a blocking group; and R is releaseable therefrom by enzymatic hydrolysis to form a measureable amount of 7-amino-4-trifluoromethylcoumarin.
Claim 3
Dependent Claim
20 words
The substrate of claim 1, wherein R is an amino acid chain comprising from 1 to about 12 amino acids.
Claim 4
Dependent Claim
20 words
The substrate of claim 1, wherein R is an amino acid chain comprising from 1 to about 6 amino acids.
Claim 5
Dependent Claim
9 words
The substrate of claim 1 wherein R is gly-gly-arg-.
Claim 6
Dependent Claim
9 words
The substrate of claim 1, wherein R is d-ala-leu-lys-.
Claim 7
Dependent Claim
9 words
The substrate of claim 1, wherein R is leu-.
Claim 8
Dependent Claim
9 words
The substrate of claim 1, wherein R is Cbz-Val-Lys-Lys-Arg-.
Claim 9
Dependent Claim
24 words
The substrate of any one of claims 2, 3, 4, 5, or 6, wherein a blocking group is bonded to the terminal amino acid.
Claim 10
Dependent Claim
18 words
The substrate of claim 8, wherein said blocking group is carbobenzoxy, benzoyl, glutaryl, t-butylcarbonyl, d-proline, d-valine, or d-alanine.
References Cited (Backward Citations)
US3886136A
Cited
US3884896A
Cited
US4247454A
Cited
US4061625A
Cited
US4137225A
Cited
US4276375A
Cited
US4276378A
Cited
US4279810A
Cited
US4237047A
Cited
US4257939A
Cited
US4217269A
Cited
WO1980002295A1
Cited
US4308202A
Cited
US4244865A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-10 | 1987-04-11 | 1987-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-10 | 1991-04-11 | 1991-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-10 | 1995-04-11 | 1995-10-11 | $7700.00 | Window Closed / Lapsed |