US4402819A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antibody-selective membrane electrodes
Assignee: University of Delaware
•
Filing: 1981-10-21
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Granted: 1983-09-06
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Expires: 2001-10-21
Abstract
Abstract An antibody-selective potentiometric electrode for the quantitative determination of antibodies in dilute liquid serum samples employing an insoluble membrane incorporating an antigen having bonded thereto an ion carrier effecting the permeability of preselected cations therein, which permeability is a function of specific antibody concentrations in analysis, and the corresponding method of analysis.
Inventors
- 👤 Garry A. Rechnitz
- 👤 Robert L. Solsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 04:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
88 words
In an antibody-selective membrane electrode useful for the detection of an antibody specific for a preselected hapten or antigen, said membrane electrode having a water-insoluble membrane for separating a test electrolyte from an internal electrolyte, the improvement comprising using as the separating membrane a plasticized water-insoluble solid organic polymer having dispersed therein a cation-selective crown-ether to which the hapten or antigen is covalently bound, wherein said antibody is capable of binding to said antigen or said hapten thereby producing a change in the cation selectivity of said membrane.
Claim 2
Dependent Claim
88 words
In an antibody-selective membrane electrode useful for the detection of an antibody specific for a preselected hapten or antigen, said membrane electrode having a water-insoluble membrane for separating a test electrolyte from an internal electrolyte, the improvement comprising using as the separating membrane a plasticized water-insoluble solid organic polymer having dispersed therein a cation-selective crown-ether to which the hapten or antigen is covalently bound, wherein said antibody is capable of binding to said antigen or said hapten thereby producing a change in the cation selectivity of said membrane.
Claim 3
Dependent Claim
13 words
The electrode of claim 1 in which the crown-ether is a dibenzo-18-crown-6 ether.
Claim 4
Dependent Claim
13 words
The electrode of claim 1 in which the hapten is a dinitrophenyl group.
Claim 5
Dependent Claim
13 words
The electrode of claim 1 in which the antigen is bovine serum albumin.
Claim 6
Dependent Claim
25 words
The electrode of claim 1 in which the polymeric component of the membrane is selected from the group consisting of polyvinyl chloride and triacetyl cellulose.
Claim 7
Dependent Claim
19 words
The electrode of claim 5 in which the solid organic polymer is polyvinyl chloride plasticized with an alkyl sebacate.
Claim 8
Dependent Claim
18 words
The electrode of claim 6 in which the solid organic polymer is polyvinyl chloride plasticized with dibutyl sebacate.
Claim 9
Dependent Claim
18 words
The electrode of claim 5 in which the solid organic polymer is triacetyl cellulose plasticized with p-nitroethyl benzene.
References Cited (Backward Citations)
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US4081334A
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US4276141A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-09-05 | 1987-03-07 | 1987-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-09-05 | 1991-03-07 | 1991-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-09-05 | 1995-03-07 | 1995-09-06 | $7700.00 | Window Closed / Lapsed |