Patent US4394487A successfully refreshed from Google Patents.
US4394487A
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US
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Adsorbent for the affinity-specific separation of macromolecular materials
Assignee: Roche Diagnostics GmbH
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Filing: 1981-10-07
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Granted: 1983-07-19
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Expires: 2001-10-07
Abstract
Abstract Adsorbents are provided for the affinity-specific separation of macromolecular materials, particularly biopolymers, the adsorbents comprising a polymeric carrier on to which an affine residue for the macromolecular material is covalently bound, either directly or via polymeric spacer. Preferred affine residue is comprised of a specific complex formers which are base-specific and/or structure specific for the macromolecular material. The invention also provides novel complex formers comprising a certain dyestuff residues and processes for their preparation.
Inventors
- 👤 Werner Muller
- 👤 Hans Bunemann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:15
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3255; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3253; C; C07; C07D; C07D219/00; C07D219/04; C07D219/08; C07D219/10; C07D219/12; C07D221/00; C07D221/02; C07D221/04; C07D221/06; C07D221/10; C07D221/12; C08; C08F; C08F265/00; C08F265/10; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12N15/101
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
124 words
Process for the preparation of an adsorbent for the affinity-specific separation of nucleic acids, comprising a polymeric carrier material onto which an affine residue group consisting of a base and/or structure specific complex former for nucleic acids, is covalently bound wherein said polymeric carrier material is first formed from at least one monomer which additionally possesses a functional group via which the affine residue for the biopolymer can be bound directly or via a polymeric spacer, the polymeric carrier material is optionally comminuted to the desired particle size and then the affine residue for the biopolymer is grafted on either directly or by copolymerization in the presence of at least one further monomer as co-monomer so that particles of the desired size are produced.
Claim 2
Dependent Claim
124 words
Process for the preparation of an adsorbent for the affinity-specific separation of nucleic acids, comprising a polymeric carrier material onto which an affine residue group consisting of a base and/or structure specific complex former for nucleic acids, is covalently bound wherein said polymeric carrier material is first formed from at least one monomer which additionally possesses a functional group via which the affine residue for the biopolymer can be bound directly or via a polymeric spacer, the polymeric carrier material is optionally comminuted to the desired particle size and then the affine residue for the biopolymer is grafted on either directly or by copolymerization in the presence of at least one further monomer as co-monomer so that particles of the desired size are produced.
Claim 3
Dependent Claim
36 words
Process as claimed in claim 1 wherein the affine residue for the biopolymer having a copolymerizable double bound is grafted on to poly-bisacrylamide in the presence of an excess of a further copolymerizable monomer as co-monomer.
Claim 4
Dependent Claim
28 words
Process as claimed in claim 1 wherein the graft copolymerization is carried out in water or in an inert solvent in the presence of a conventional polymerization catalyst.
Claim 5
Dependent Claim
33 words
Process as claimed in claim 1 wherein the affine residue for the biopolymer having a copolymerizable double bond is acrylic-phenyl neutral red or acrylic-malachite green and the further copolymerizable monomer used is acrylamide.
Claim 6
Dependent Claim
30 words
Process as claimed in claim 1 wherein a copolymer of acrylamide and acrylic-phenyl neutral red or acrylic-malachite green with a degree of polymerization of 200 to 300 is grafted on.
Claim 7
Dependent Claim
33 words
Process as claimed in claim 1 wherein the affine residue for the biopolymer having a copolymerizable double bond and the further copolymerizable monomer are used in a weight ratio of 1:100 to 1:5000.
Claim 8
Dependent Claim
14 words
Process as claimed in claim 6 wherein the said weight ratio is preferably 1:3000.
References Cited (Backward Citations)
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US4017476A
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US4046750A
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US4035316A
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US4213860A
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US4194877A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-18 | 1987-01-17 | 1987-07-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-18 | 1991-01-17 | 1991-07-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-18 | 1995-01-17 | 1995-07-19 | $7700.00 | Window Closed / Lapsed |