Patent US4392933A successfully refreshed from Google Patents.
US4392933A
Expired / Lapsed
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Electrochemical measuring apparatus comprising enzyme electrode
Assignee: Panasonic Holdings Corp
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Filing: 1982-03-15
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Granted: 1983-07-12
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Expires: 2002-03-15
Abstract
Abstract An immobilized enzyme electrode effective in measurement of the substrate concentration of the enzyme and in conversion from enzyme reaction energies into electric energies. The immobilized enzyme, of an oxidase system, such as glucose oxidase, amino acid oxidase, xanthine oxidase or the like and a metal oxide capable of constituting a redox system which is reduced through coupling with these enzyme reactions and is electrochemically oxidized (anodic oxidation) are combined with each other. The use of the enzyme electrode allows the determination quantity of the enzyme substrate as extremely low as approximately 10 -5 to 10 -6 mole/l in concentration.
Inventors
- 👤 Kenichi Nakamura
- 👤 Shiro Nankai
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 04:47
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
157 words
An electrochemical measuring system for measuring the concentration of a substrate in solution comprising a counter electrode connected to a reference electrode and a solid state enzyme electrode, said solid state enzyme electrode comprising an electron collector, a metal oxide in intimate contact with the electron collector and an oxidase immobilized onto said metal oxide, said electron collector connected to means which make it an anode, wherein said metal oxide is capable of being reduced through an oxidase reaction and oxidized electrochemically through contact with the electron collector; said system further comprising a means for applying a constant potential between said enzyme electrode and said reference electrode and comprising a means for measuring the variation of current flowing between the enzyme electrode and the counter electrode when the system is used to measure the concentration of a substrate as a result of said electrochemical reaction, said measured current corresponding to said concentration of enzyme to be measured.
Claim 2
Dependent Claim
157 words
An electrochemical measuring system for measuring the concentration of a substrate in solution comprising a counter electrode connected to a reference electrode and a solid state enzyme electrode, said solid state enzyme electrode comprising an electron collector, a metal oxide in intimate contact with the electron collector and an oxidase immobilized onto said metal oxide, said electron collector connected to means which make it an anode, wherein said metal oxide is capable of being reduced through an oxidase reaction and oxidized electrochemically through contact with the electron collector; said system further comprising a means for applying a constant potential between said enzyme electrode and said reference electrode and comprising a means for measuring the variation of current flowing between the enzyme electrode and the counter electrode when the system is used to measure the concentration of a substrate as a result of said electrochemical reaction, said measured current corresponding to said concentration of enzyme to be measured.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-11 | 1987-01-10 | 1987-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-11 | 1991-01-10 | 1991-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-11 | 1995-01-10 | 1995-07-12 | $7700.00 | Window Closed / Lapsed |