Method and apparatus for governing the reaction rate of enzymatic reactions

Claim 1

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: a. providing said enzymatic electrode with a solid, inert, working electrode and with a particulate mass of discrete enzymatic particles, each formed essentially of said conductive support medium having said enzyme fixed to the surface thereof; b. placing said enzymatic electrode in a reaction space wherein said particulate mass of enzymatic particles is arranged for electrical contact thereof with said working electrode; c. circulating said liquid reaction system within said reaction space; and d. applying said adjustable potential, via said working electrode, to the conductive support medium of said enzymatic particles.

Claim 2

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: a. providing said enzymatic electrode with a solid, inert, working electrode and with a particulate mass of discrete enzymatic particles, each formed essentially of said conductive support medium having said enzyme fixed to the surface thereof; b. placing said enzymatic electrode in a reaction space wherein said particulate mass of enzymatic particles is arranged for electrical contact thereof with said working electrode; c. circulating said liquid reaction system within said reaction space; and d. applying said adjustable potential, via said working electrode, to the conductive support medium of said enzymatic particles.

Claim 5

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: applying to said support medium a variable potential alternating between two levels, while adjusting the mean value thereof so as to provide a corresponding mean catalytic activity of said enzyme for transforming said substrate at a given rate.

Claim 6

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: applying to said support medium a variable potential alternating between two levels, while adjusting the mean value thereof so as to provide a corresponding mean catalytic activity of said enzyme for transforming said substrate at a given rate.

Claim 7

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: a. providing said enzymatic electrode with a solid inert working electrode and with a particulate mass of discrete enzymatic particles, each formed essentially of said conductive support medium having said enzyme fixed to the surface thereof; b. placing said enzymatic electrode in a reaction space wherein said particulate mass of enzymatic particles is arranged for electrical contact thereof with said working electrode; c. circulating said liquid reaction system within said reaction space; and d. applying an alternating potential via said working electrode to the support medium of said enzymatic particles, while adjusting the mean value of said potential so as to provide a corresponding mean catalytic activity of said enzyme fixed to said particles, whereby to transform said substrate at a given rate.

Claim 8

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: a. providing said enzymatic electrode with a solid inert working electrode and with a particulate mass of discrete enzymatic particles, each formed essentially of said conductive support medium having said enzyme fixed to the surface thereof; b. placing said enzymatic electrode in a reaction space wherein said particulate mass of enzymatic particles is arranged for electrical contact thereof with said working electrode; c. circulating said liquid reaction system within said reaction space; and d. applying an alternating potential via said working electrode to the support medium of said enzymatic particles, while adjusting the mean value of said potential so as to provide a corresponding mean catalytic activity of said enzyme fixed to said particles, whereby to transform said substrate at a given rate.

Claim 10

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: a. providing said enzymatic electrode with a solid, inert, working electrode and with a particulate mass of discrete enzymatic particles each formed essentially of said conductive support medium having said enzyme fixed to the surface thereof; b. placing said enzymatic electrode in a reaction space wherein said particulate mass is arranged to provide an agitated bed of said enzymatic particles whereby to provide repeated contact thereof with said working electrode; c. circulating said liquid rection system in said reaction space so as to provide said agitated bed of enzymatic particles in suspension in said liquid system, whereby each suspended particle in said agitated bed undergoes continually repeated impingements on said working electrode; and d. applying said adjustable potential to said working electrode so as to thereby apply said potential to the support medium of each suspended enzymatic particle by intermittent contact thereof during said continually repeated impingements thereof on said working electrode, each said suspended particle being at a different potential level while undergoing movement within said agitated bed, between successive impingements thereof on said working electrode.

Claim 11

A method of governing the reaction rate of enzymatic reactions for continuously transforming a substrate by contact with an enzyme fixed to a solid carrier, comprising the steps of: bringing an electrically conductive liquid reaction system, containing at least one substrate to be transformed, into contact with an enzymatic electrode comprising at least one enzyme fixed to an electrically conductive solid support medium; and regulating the catalytic activity of said enzyme by applying an electric potential to said support medium while adjusting the value of said potential so as to transform said substrate at a given rate, said method further comprising the improvements consisting of: a. providing said enzymatic electrode with a solid, inert, working electrode and with a particulate mass of discrete enzymatic particles each formed essentially of said conductive support medium having said enzyme fixed to the surface thereof; b. placing said enzymatic electrode in a reaction space wherein said particulate mass is arranged to provide an agitated bed of said enzymatic particles whereby to provide repeated contact thereof with said working electrode; c. circulating said liquid rection system in said reaction space so as to provide said agitated bed of enzymatic particles in suspension in said liquid system, whereby each suspended particle in said agitated bed undergoes continually repeated impingements on said working electrode; and d. applying said adjustable potential to said working electrode so as to thereby apply said potential to the support medium of each suspended enzymatic particle by intermittent contact thereof during said continually repeated impingements thereof on said working electrode, each said suspended particle being at a different potential level while undergoing movement within said agitated bed, between successive impingements thereof on said working electrode.

Claim 14

An apparatus for governing the reaction rate of an enzymatic reaction for continuously transforming a substrate contained in an electrically conductive liquid system, comprising: a. at least one enzymatic electrode having at least one enzyme fixed to the surface of a solid, electronically conductive support associated with means for applying an adjustable potential to said electrode; b. a reaction chamber which is equipped with an enzymatic electrode and with an inert counter-electrode and is adapted to receive said liquid reaction system and to discharge the products of the enzymatic reaction; and c. adjustable electrical supply and voltage control means connected to said enzymatic electrode and to said counterelectrode and adapted to apply between said electrodes a potential difference which is adjustable so as to regulate the catalytic activity of said enzyme; the improvement wherein: said enzymatic electrode comprises a particulate enzymatic mass, composed of discrete enzymatic particles, each essentially formed of said solid conductive support having said enzyme fixed thereto, and further comprises a solid, inert working electrode, operatively associated with said mass and with said electrical supply and voltage control means, so as to subject the particles of said mass to an adjustable potential; said apparatus further comprises circulating means adapted to circulate said liquid reaction system within said reaction chamber so as to provide intimate contact thereof with each particle of said enzymatic mass, the particles of said mass being subjected to said adjustable potential during said circulation.

Claim 15

An apparatus for governing the reaction rate of an enzymatic reaction for continuously transforming a substrate contained in an electrically conductive liquid system, comprising: a. at least one enzymatic electrode having at least one enzyme fixed to the surface of a solid, electronically conductive support associated with means for applying an adjustable potential to said electrode; b. a reaction chamber which is equipped with an enzymatic electrode and with an inert counter-electrode and is adapted to receive said liquid reaction system and to discharge the products of the enzymatic reaction; and c. adjustable electrical supply and voltage control means connected to said enzymatic electrode and to said counterelectrode and adapted to apply between said electrodes a potential difference which is adjustable so as to regulate the catalytic activity of said enzyme; the improvement wherein: said enzymatic electrode comprises a particulate enzymatic mass, composed of discrete enzymatic particles, each essentially formed of said solid conductive support having said enzyme fixed thereto, and further comprises a solid, inert working electrode, operatively associated with said mass and with said electrical supply and voltage control means, so as to subject the particles of said mass to an adjustable potential; said apparatus further comprises circulating means adapted to circulate said liquid reaction system within said reaction chamber so as to provide intimate contact thereof with each particle of said enzymatic mass, the particles of said mass being subjected to said adjustable potential during said circulation.