Patent US4391887A successfully refreshed from Google Patents.
US4391887A
Expired / Lapsed
US
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Preparations of micro-organisms
Assignee: Bayer AG
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Filing: 1981-08-13
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Granted: 1983-07-05
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Expires: 2001-08-13
Abstract
Abstract A process for the production of a storage-stable preparation of micro-organisms, starting from a mixed culture of micro-organisms, which is capable to degrade products of industrial organic syntheses, which comprises that the culture conditions of an active mixed culture enriched in the customary manner are optimized, with the addition of the product to be degraded, in the customary manner according to the rate of degradation of the product and this mixed culture is converted in at least two passes into a stabilized activated mixed culture under the optimum conditions thus determined, and the culture thereby obtained is rendered storage-stable, its activity being maintained.
Inventors
- 👤 Jorg Baumgarten
- 👤 Werner Frommer
- 👤 Delf Schmidt
- 👤 Friedrich Schmidt
- 👤 Douglas M. Munnecke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 15:23
CPC Classifications
A; A01; A01N; A01N25/00; A01N25/32; B; B09; B09C; B09C1/00; B09C1/10; B09C1/105; C; C02; C02F; C02F3/00; C02F3/34; C12; C12N; C12N1/00; C12N1/04; C12N1/20; C12N1/205; C12N1/26; C12N1/36; C12R; C12R2001/00; C12R2001/01; C12R2001/38; Y; Y10; Y10S; Y10S210/00; Y10S210/902; Y10S210/906; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
115 words
A process for the production of a storage-stable material capable of degrading a waste product comprising at least one of phosphate esters and acrylonitrile, comprising cultivating a mixed culture of microorganisms capable of growing on the waste product by feeding in the waste product at a progressively increasing concentration which does not kill the microorganisms due to by-product formation, terminating the cultivation when the waste product-splitting activity of the culture has leveled off, separating at least a portion of the now active mixed culture and repeating the cultivation at least once to produce a stabilized active mixed culture, treating the stabilized active mixed culture chemically, and then drying to obtain a storage-stable active mixed culture.
Claim 2
Dependent Claim
115 words
A process for the production of a storage-stable material capable of degrading a waste product comprising at least one of phosphate esters and acrylonitrile, comprising cultivating a mixed culture of microorganisms capable of growing on the waste product by feeding in the waste product at a progressively increasing concentration which does not kill the microorganisms due to by-product formation, terminating the cultivation when the waste product-splitting activity of the culture has leveled off, separating at least a portion of the now active mixed culture and repeating the cultivation at least once to produce a stabilized active mixed culture, treating the stabilized active mixed culture chemically, and then drying to obtain a storage-stable active mixed culture.
Claim 3
Dependent Claim
23 words
A process according to claim 1, wherein the microorganisms are protozoa, algae, blue-green algae, yeasts, fungi, bacteria and mixtures of any of these.
Claim 4
Dependent Claim
85 words
A process according to claim 1, wherein the culture conditions include a temperature of 10°-70° C., a pH-value of 1-10, a phosphate (PO 4 3- ) content of 0.08-10 g/liter, a nitrogen salt content (as NH 4 Cl) of 0-50 g/liter, a magnesium content (as MgSO 4 ) of 0.01-2 g/liter, an iron salt content of 0.04-0.1 g/liter, a calcium content (as CaCl 2 ) of 0.00-0.1 g/liter, an oxygen concentration of 0-48 mg/liter, and a culture period of between 1 hour and 14 days.
Claim 5
Dependent Claim
20 words
A process according to claim 1, wherein an emulsifying agent and/or a dispersing agent are present in the culture medium.
Claim 6
Dependent Claim
17 words
A process according to claim 1, wherein the conversion is effected in from 2 to 50 passes.
Claim 7
Dependent Claim
38 words
A process according to claim 1, wherein the stabilized activated mixed culture is rendered storage-stable by freezing, by lyophilisation, by the removal of water with an organic solvent, by spray-drying or by the addition of a preserving chemical.
Claim 8
Dependent Claim
29 words
A process according to claim 1, wherein the mixed culture comprises a mixture of protozoa, algae, blue-green algae, yeast, fungi or bacteria with one another or with each other.
Claim 9
Dependent Claim
21 words
A process according to claim 1, wherein the initial mixed culture is a storage-stable active mixed culture from a prior cycle.
Claim 10
Dependent Claim
9 words
The product produced by the process of claim 1.
Claim 11
Dependent Claim
40 words
A process according to claim 1, wherein the culture medium is a yeast extract, yeast autolysate or meat extract medium with or without an additional other source of carbon and/or nitrogen and as organic acids, amino acids, carbohydrates or nitrophenol.
Claim 12
Dependent Claim
14 words
A process according to claim 1, wherein the waste product comprises a phosphate ester.
Claim 13
Dependent Claim
12 words
A process according to claim 1, wherein the waste product comprises acrylonitrile.
Claim 14
Dependent Claim
29 words
A biologically pure culture of Pseudomonas strain DSM 1192 or 1193, said culture being capable of forming a stabilized mixed culture capable of degrading products of industrial organic syntheses.
Claim 15
Dependent Claim
29 words
A biologically pure culture of Pseudomonas strain DSM 1192 or 1193, said culture being capable of forming a stabilized mixed culture capable of degrading products of industrial organic syntheses.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-04 | 1987-01-03 | 1987-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-04 | 1991-01-03 | 1991-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-04 | 1995-01-03 | 1995-07-05 | $7700.00 | Window Closed / Lapsed |