US4094097A
Expired / Lapsed
US
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Method for preparing a pesticidally resistant rhizobium and agronomic composition thereof
Assignee: Cornell Research Foundation Inc
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Filing: 1976-09-10
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Granted: 1978-06-13
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Expires: 1996-09-10
Abstract
Abstract This invention relates to Rhizobium strains having good infecting and nitrogen-fixing characteristics and which are resistant to fungicides. These strains are produced by cultivating a Rhizobium strain sensitive to a particular fungicide in the presence of an amount of the fungicide, and for a time less than that sufficient to kill the entire Rhizobium population, but sufficient to kill a majority of the population. The remaining Rhizobium population is isolated and recultured in the presence of an increased amount of the fungicide, again an amount and a time less than sufficient to kill the entire Rhizobium population, but sufficient to kill a majority of the population. This procedure is repeated for sufficient passages, with increasing amounts of said fungicide, to provide a Rhizobium strain sufficiently resistant to said fungicide so that the Rhizobium strain multiplies and enters into a nitrogen-fixing symbiosis in the presence of agriculturally effective amounts of the fungicide.
Inventors
- 👤 Martin Alexander
- 👤 Oluwasuyi Odeyemi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 15:22
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N1/36; C12R; C12R2001/00; C12R2001/01; C12R2001/41; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/878
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
156 words
A method for the preparation of a bacteria preparation intended for inoculation of leguminous seed, which preparation contains a fungicide resistant strain of Rhizobium, which strain has good infecting and nitrogen-fixing abilities in symbiosis with plants of the leguminosea family which method comprises: cultivating, in the presence of a Rhizobium medium and an amount of a selected fungicide sufficient to kill at least 90% of the Rhizobium culture initially present, a culture of Rhizobium bacteria of selected strain and inoculation group having good infecting and nitrogen-fixing ability, whereby bacteria strains and sensitive to said amount of said fungicide are killed; subjecting the residual fungicide resistant bacteria to at least one additional cultivation step, as above, in the presence of an increased amount of said fungicide; and recovering a Rhizobium strain having increased resistance to said fungicide; said fungicide being selected from agricultrually useful legume seed or soil fungicides which adversely affect the Rhizobium bacteria being cultivated.
Claim 2
Independent Claim
156 words
A method for the preparation of a bacteria preparation intended for inoculation of leguminous seed, which preparation contains a fungicide resistant strain of Rhizobium, which strain has good infecting and nitrogen-fixing abilities in symbiosis with plants of the leguminosea family which method comprises: cultivating, in the presence of a Rhizobium medium and an amount of a selected fungicide sufficient to kill at least 90% of the Rhizobium culture initially present, a culture of Rhizobium bacteria of selected strain and inoculation group having good infecting and nitrogen-fixing ability, whereby bacteria strains and sensitive to said amount of said fungicide are killed; subjecting the residual fungicide resistant bacteria to at least one additional cultivation step, as above, in the presence of an increased amount of said fungicide; and recovering a Rhizobium strain having increased resistance to said fungicide; said fungicide being selected from agricultrually useful legume seed or soil fungicides which adversely affect the Rhizobium bacteria being cultivated.
Claim 3
Dependent Claim
19 words
The method, as in claim 1, wherein the resultant Rhizobium strain is resistant to agriculturally significant amounts of fungicide.
Claim 4
Dependent Claim
26 words
The method, as in claim 2, wherein the bacteria are subjected to at least 6 cultivating steps in the presence of increasing amounts of said fungicide.
Claim 5
Dependent Claim
20 words
The method, as in claim 3, wherein the fungicide is selected from the group consisting of thiram, spergon and phygon.
Claim 6
Dependent Claim
40 words
The method, as in claim 1, wherein at least 99% of the bacteria in the initial culture are killed in the course of each cultivating step, and wherein the resultant Rhizobium strain is resistant to agriculturally significant amounts of fungicide.
Claim 7
Dependent Claim
25 words
The method, as in claim 5, where the fungicide is selected from the group consisting of carbamic acid derivatives, thiram disulfides and halogenated organic compounds.
Claim 8
Dependent Claim
20 words
The method, as in claim 6, where the fungicide is selected from the group consisting of thiram, spergon and phygon.
Claim 9
Dependent Claim
18 words
A leguminous seed having coated thereon a symbiotically-compatible, fungicide-resistance Rhizobium strain produced by the process of claim 1.
References Cited (Backward Citations)
US3168796A
Cited
US3616236A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-06-12 | 1981-12-12 | 1982-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-06-12 | 1985-12-12 | 1986-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-06-12 | 1989-12-12 | 1990-06-13 | $7700.00 | Window Closed / Lapsed |