Patent US4390619A successfully refreshed from Google Patents.
US4390619A
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Leukocyte or platelet storage using ion-exchange resins
Assignee: Individual
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Filing: 1981-09-28
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Granted: 1983-06-28
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Expires: 2001-09-28
Abstract
Abstract A method of storing and preserving the shelf life of leukocytes or platelets suitable for transfusion is extended by maintaining viable platelets having adequate ATP and pH levels using an insoluble polymer material as a source of weakly basic buffer ions during the storage period.
Inventors
- 👤 Denise Harmening-Pittiglio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:47
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/15
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
85 words
In a composition of matter consisting essentially of metabolizing leukocytes or platelets in an in vitro environment capable of supporting platelet metabolism, the improvement which comprises a water-insoluble polymer containing releasable phosphate or bicarbonate ions for supplying a physiologically acceptable, continuous, sustained release source of buffer to said platelets in an amount sufficient to maintain both pH and ATP levels equal to at least 60% of those of freshly prepared platelets for a storage period of at least 7 days of storage at 22° C.
Claim 2
Dependent Claim
85 words
In a composition of matter consisting essentially of metabolizing leukocytes or platelets in an in vitro environment capable of supporting platelet metabolism, the improvement which comprises a water-insoluble polymer containing releasable phosphate or bicarbonate ions for supplying a physiologically acceptable, continuous, sustained release source of buffer to said platelets in an amount sufficient to maintain both pH and ATP levels equal to at least 60% of those of freshly prepared platelets for a storage period of at least 7 days of storage at 22° C.
Claim 3
Dependent Claim
16 words
A composition according to claim 1, wherein said environment includes packed platelets substantially free of leukocytes.
Claim 4
Dependent Claim
16 words
A composition according to claim 2 wherein said environment includes packed platelets substantially free of erythocytes.
Claim 5
Dependent Claim
12 words
A composition according to claim 1 wherein said water-insoluble polymer is particulate.
Claim 6
Dependent Claim
14 words
A composition according to claim 4, wherein said polymer is an ion exchange resin.
Claim 7
Dependent Claim
16 words
A composition according to claim 5, wherein said polymer is a weakly basic anion exchange resin.
Claim 8
Dependent Claim
17 words
A composition according to claim 6 wherein said buffer is inorganic dibasic phosphate HPO 4 -- .
Claim 9
Dependent Claim
12 words
A composition according to claim 7 wherein said platelets are human platelets.
Claim 10
Dependent Claim
18 words
A composition according to claim 7 wherein said in vitro environment is within a sterile platelet collection container.
Claim 11
Dependent Claim
83 words
In an article of manufacture comprising a sterile platelet collection and storage container, the improvement which comprises a water-insoluble polymer containing releaseable phosphate or bicarbonate ions for supplying a continuous, sustained release source of buffer to metabolizing leukocytes or platelets in an amount sufficient to maintain both pH and ATP levels of said leukocytes or platelets at least equal to 60% of those levels in freshly prepared cells for a storage period equivalent to at least 7 days of storage at 22° C.
Claim 12
Dependent Claim
83 words
In an article of manufacture comprising a sterile platelet collection and storage container, the improvement which comprises a water-insoluble polymer containing releaseable phosphate or bicarbonate ions for supplying a continuous, sustained release source of buffer to metabolizing leukocytes or platelets in an amount sufficient to maintain both pH and ATP levels of said leukocytes or platelets at least equal to 60% of those levels in freshly prepared cells for a storage period equivalent to at least 7 days of storage at 22° C.
Claim 13
Dependent Claim
13 words
An article according to claim 10 wherein said water insoluble polymer is particulate.
Claim 14
Dependent Claim
13 words
An article according to claim 11, wherein said polymer is an ion-exchange resin.
Claim 15
Dependent Claim
16 words
An article according to claim 12, wherein said polymer is a weakly basic anion exchange resin.
Claim 16
Dependent Claim
82 words
In a method for maintaining metabolizing leukocytes or platelets suitable for transfusion in a sterile container, the improvement which comprises added to said container a water-insoluble polymer containing releasable phosphate or bicarbonate ions for supplying a continuous source of buffer to said metabolizing cells in an amount sufficient to maintain both pH and ATP levels equal to at least 60% of those levels in freshly prepared cells for a storage period equivalent to at least 7 days of storage at 22° C.
Claim 17
Dependent Claim
82 words
In a method for maintaining metabolizing leukocytes or platelets suitable for transfusion in a sterile container, the improvement which comprises added to said container a water-insoluble polymer containing releasable phosphate or bicarbonate ions for supplying a continuous source of buffer to said metabolizing cells in an amount sufficient to maintain both pH and ATP levels equal to at least 60% of those levels in freshly prepared cells for a storage period equivalent to at least 7 days of storage at 22° C.
Claim 18
Dependent Claim
12 words
A method according to claim 14 wherein said water-insoluble polymer is particulate.
Claim 19
Dependent Claim
14 words
A method according to claim 15 wherein said polymer is an ion exchange resin.
Claim 20
Dependent Claim
16 words
A method according to claim 16 wherein said polymer is a weakly basic anion exchange resin.
Claim 21
Dependent Claim
48 words
A storable, transfusable composition of matter comprising metabolizing leukocytes or platelets in an in vitro environment substantially free of erythrocytes and capable of supporting cellular metabolism having incorporated therein a water-insoluble polymer containing releasable phosphate or bicarbonate ions capable of continuously supplying weakly basic buffer to said cells.
Claim 22
Dependent Claim
48 words
A storable, transfusable composition of matter comprising metabolizing leukocytes or platelets in an in vitro environment substantially free of erythrocytes and capable of supporting cellular metabolism having incorporated therein a water-insoluble polymer containing releasable phosphate or bicarbonate ions capable of continuously supplying weakly basic buffer to said cells.
Claim 23
Dependent Claim
12 words
A composition according to claim 18 wherein said water-insoluble polymer is particulate.
Claim 24
Dependent Claim
14 words
A composition according to claim 19 wherein said polymer is an ion exchange resin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-27 | 1986-12-27 | 1987-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-27 | 1990-12-27 | 1991-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-27 | 1994-12-27 | 1995-06-28 | $7700.00 | Window Closed / Lapsed |