Patent US4386069A successfully refreshed from Google Patents.
US4386069A
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Additive solution and method for preserving normal red cell morphology in whole blood during storage
Assignee: Baxter International Inc
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Filing: 1981-12-02
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Granted: 1983-05-31
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Expires: 2001-12-02
Abstract
Abstract An additive solution, which can be dispersed in whole blood to enhance preservation of normal red cell morphology during storage, comprises a fatty ester which includes at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each. The solution can be introduced into the whole blood either at the outset of the storage period or any time during the period in an amount to provide, after its introduction, a concentration of the fatty ester of between 150 and 3,000 micrograms per milliliter of whole blood. When introduced at the outset of storage, morphological changes in the red cells are significantly retarded during the storage period. When introduced at later stages of storage, the solution serves to rejuvenate a significant percentage of red cells which have undergone morphological changes prior to the introduction of the solution.
Inventors
- 👤 Timothy N. Estep
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 14:44
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
57 words
An additive solution for preserving normal red blood cell morphology during whole blood storage, said solution comprising a fatty ester including at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each, said fatty ester being present in a concentration of between 150 and 3,000 micrograms per milliliter of whole blood.
Claim 2
Dependent Claim
57 words
An additive solution for preserving normal red blood cell morphology during whole blood storage, said solution comprising a fatty ester including at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each, said fatty ester being present in a concentration of between 150 and 3,000 micrograms per milliliter of whole blood.
Claim 3
Dependent Claim
27 words
An additive solution according to claim 1 wherein said fatty ester is present in a concentration of between 250 and 500 micrograms per milliliter of whole blood.
Claim 4
Dependent Claim
20 words
An additive solution according to claim 1 or 2 wherein said fatty ester is in the form of an emulsion.
Claim 5
Dependent Claim
19 words
An additive solution according to claim 1 or 2 and further including, as part of said solution, an anticoagulant.
Claim 6
Dependent Claim
21 words
An additive solution according to claim 4 and further including, as part of said solution, a nutrient for the whole blood.
Claim 7
Dependent Claim
60 words
An additive solution for rejuvenating red blood cells which have undergone morphological changes during whole blood storage, said solution comprising a fatty ester including at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each, said fatty ester being present in a concentration of between 150 and 3,000 micrograms per milliliter of whole blood.
Claim 8
Dependent Claim
60 words
An additive solution for rejuvenating red blood cells which have undergone morphological changes during whole blood storage, said solution comprising a fatty ester including at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each, said fatty ester being present in a concentration of between 150 and 3,000 micrograms per milliliter of whole blood.
Claim 9
Dependent Claim
27 words
An additive solution according to claim 6 wherein said fatty ester is present in a concentration of between 250 and 500 micrograms per milliliter of whole blood.
Claim 10
Dependent Claim
20 words
An additive solution according to claim 6 or 7 wherein said fatty ester is in the form of an emulsion.
Claim 11
Dependent Claim
16 words
A solution according to claim 1 or 6 wherein said fatty ester is a phosphate ester.
Claim 12
Dependent Claim
14 words
A solution according to claim 9 wherein said phosphate ester has three alkyl groups.
Claim 13
Dependent Claim
16 words
A solution according to claim 1 or 6 wherein said fatty ester is a phthalate diester.
Claim 14
Dependent Claim
15 words
A solution according to claim 1 or 6 wherein said fatty hydrocarbon groups are alkyl.
Claim 15
Dependent Claim
21 words
A solution according to claim 1 or 6 wherein said fatty hydrocarbon groups each contain from seven to ten carbon atoms.
Claim 16
Dependent Claim
25 words
A solution according to claim 1 or 6 wherein said fatty ester comprises a pair of ester linkages bound to a highly mobile hydrocarbon chain.
Claim 17
Dependent Claim
17 words
A solution according to claim 14 wherein said highly mobile hydrocarbon chain is a linear alkylene chain.
Claim 18
Dependent Claim
24 words
A solution according to claim 15 wherein said linear alkylene chain contains no more than eight carbon atoms excluding the ester linkage carbon atoms.
Claim 19
Dependent Claim
18 words
A solution according to claim 1 or 6 wherein said ester linkages are bonded to adjacent carbon atoms.
Claim 20
Dependent Claim
15 words
A solution according to claim 1 or 6 wherein said fatty ester is a dioctylphthalate.
Claim 21
Dependent Claim
12 words
A solution according to claim 18 wherein said fatty ester is di-2-ethylhexylphthalate.
Claim 22
Dependent Claim
69 words
A whole blood storage system comprising a container having an interior for receiving whole blood for storage, a solution carried within said interior and including a fatty ester including at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each, said fatty ester being present in a concentration of between 150 and 3,000 micrograms per milliliter of whole blood stored within said container.
Claim 23
Dependent Claim
69 words
A whole blood storage system comprising a container having an interior for receiving whole blood for storage, a solution carried within said interior and including a fatty ester including at least two ester linkages comprising fatty hydrocarbon groups of about four to twelve carbon atoms each, said fatty ester being present in a concentration of between 150 and 3,000 micrograms per milliliter of whole blood stored within said container.
Claim 24
Dependent Claim
29 words
A whole blood storage system according to claim 20 wherein said fatty ester is present in a concentration of between 250 and 500 micrograms per milliliter of whole blood.
Claim 25
Dependent Claim
22 words
A whole blood storage system according to claim 20 or 21 wherein said fatty ester is in the form of an emulsion.
Claim 26
Dependent Claim
22 words
A whole blood storage system according to claim 20 or 21 wherein said solution further includes, as a part thereof, an anticoagulant.
Claim 27
Dependent Claim
24 words
A whole blood storage system according to claim 23 wherein said solution further includes, as a part thereof, a nutrient for the whole blood.
Claim 28
Dependent Claim
42 words
A whole blood storage system according to claim 24 wherein said container includes a donor tube having an end attached in flow communication with said interior and an opposite end, and a phlebotomy needle attached in flow communication with said opposite end.
Claim 29
Independent Claim
58 words
A method for storing whole blood so as to preserve normal red blood morphology during the storage period, said method comprising the step of introducing for each milliliter of whole blood present between 150 and 3,000 micrograms of a fatty ester containing at least two ester linkages comprising fatty hydrocarbon groups of four to twelve carbon atoms each.
Claim 30
Independent Claim
58 words
A method for storing whole blood so as to preserve normal red blood morphology during the storage period, said method comprising the step of introducing for each milliliter of whole blood present between 150 and 3,000 micrograms of a fatty ester containing at least two ester linkages comprising fatty hydrocarbon groups of four to twelve carbon atoms each.
Claim 31
Dependent Claim
23 words
A method according to claim 26 and further including, prior to said fatty ester introduction step, the step of emulsifying the fatty ester.
Claim 32
Dependent Claim
33 words
A method according to claim 26 or 27 wherein said fatty ester introduction step includes introducing for each milliliter of whole blood present between 250 and 500 micrograms per milliliter the fatty ester.
Claim 33
Dependent Claim
26 words
A method according to claim 26 or 27 wherein said fatty ester introduction step includes introducing the fatty ester at the outset of the storage period.
Claim 34
Dependent Claim
29 words
A method according to claim 26 or 27 wherein said fatty ester introduction step includes introducing the fatty ester at any time after the outset of the storage period.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1986-05-30 | 1986-11-29 | 1987-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-30 | 1990-11-29 | 1991-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-30 | 1994-11-29 | 1995-05-31 | $7700.00 | Window Closed / Lapsed |