Patent US4378344A successfully refreshed from Google Patents.
US4378344A
Expired / Lapsed
US
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Method and apparatus for performing multiple, simultaneous in vitro diagnostic tests using a solid phase system
Assignee: Ventrex Laboratories Inc
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Filing: 1979-09-28
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Granted: 1983-03-29
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Expires: 1999-09-28
Abstract
Abstract A method for performing multiple, simultaneous in vitro diagnostic tests is provided. The method utilizes a solid phase device comprising a receptacle and an insert. The receptacle has one or more fixed components immobilized on its inner surface. The insert has one or more fixed components--different from those immobilized on the receptacle--immobilized on its surface which is in contact with a fluid sample when inserted therein. The test is performed by placing a fluid sample, having two or more mobile components reactive with the fixed components, into the receptacle and in contact with the insert for a period of time and measuring the changes which are a function of the concentration of the mobile components.
Inventors
- 👤 Richard J. Zahradnik
- 👤 Roger N. Piasio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 06:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; Y; Y10; Y10S; Y10S435/00; Y10S435/973; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
210 words
A method for simultaneously assaying a fluid sample for at least two different unknown mobile components contained therein which method comprises: (a) placing said fluid sample inside a receptacle having immobilized on its inner surface at least one fixed component reactive with at least one mobile component of the fluid sample and also containing a solid coated insert so positioned as to contact said fluid sample, which coated insert has immobilized on its surface in contact with said fluid sample at least one fixed component which is different from any fixed component immobilized on the inner surface of said receptacle and is reactive with at least one of the mobile components of the fluid sample that is unreactive with any fixed component immobilized on the inner surface of said receptacle, (b) allowing the mobile components of the fluid sample to react with the fixed components immobilized on the receptacle inner surface and on that surface of the solid insert in contact with the sample for a predetermined time period and, (c) determining the concentration of each reactive mobile component originally present in said fluid sample based on the amount of each of said reactive mobile components that reacted with each of said reactive fixed components during said predetermined time period.
Claim 2
Independent Claim
210 words
A method for simultaneously assaying a fluid sample for at least two different unknown mobile components contained therein which method comprises: (a) placing said fluid sample inside a receptacle having immobilized on its inner surface at least one fixed component reactive with at least one mobile component of the fluid sample and also containing a solid coated insert so positioned as to contact said fluid sample, which coated insert has immobilized on its surface in contact with said fluid sample at least one fixed component which is different from any fixed component immobilized on the inner surface of said receptacle and is reactive with at least one of the mobile components of the fluid sample that is unreactive with any fixed component immobilized on the inner surface of said receptacle, (b) allowing the mobile components of the fluid sample to react with the fixed components immobilized on the receptacle inner surface and on that surface of the solid insert in contact with the sample for a predetermined time period and, (c) determining the concentration of each reactive mobile component originally present in said fluid sample based on the amount of each of said reactive mobile components that reacted with each of said reactive fixed components during said predetermined time period.
Claim 3
Dependent Claim
35 words
The method of claim 1 in which the entirety of either the fixed component or the mobile component of each fixed component-mobile component reactive pair is labelled prior to placing said sample in said receptacle.
Claim 4
Dependent Claim
18 words
The method of claim 2 in which each of the reactive pairs is labelled with the same label.
Claim 5
Dependent Claim
18 words
The method of claim 2 in which each of the reactive pairs is labelled with a different label.
Claim 6
Dependent Claim
39 words
The method of claim 2 wherein each of the mobile components is an antigen, each of the fixed components is an antibody specific for one of said antigens and each reaction is a binding reaction of antigen to antibody.
Claim 7
Dependent Claim
38 words
The method of claim 2 in which each of said fixed components comprises an anti-antibody to which is immunologically bound an antibody to said anti-antibody, which antibody is also specifically reactive with an antigen of the fluid sample.
Claim 8
Dependent Claim
39 words
The method of claim 2 wherein said insert comprises a central rod with at least nine fin-like projections extending outwardly from said rod along a portion of the length of said rod and the receptacle is a test tube.
Claim 9
Dependent Claim
62 words
An apparatus for conducting in vitro immunoassays in accordance with claim 5 or 6 which comprises a receptacle having immobilized on its inner surface at least one fixed immunological component and a solid coated insert having immobilized on its surface at least one fixed immunological component different from and unreactive with any fixed component immobilized on the inner surface of said receptacle.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-03-28 | 1986-09-27 | 1987-03-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-03-28 | 1990-09-27 | 1991-03-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-03-28 | 1994-09-27 | 1995-03-29 | $7700.00 | Window Closed / Lapsed |