Patent US4377482A successfully refreshed from Google Patents.
US4377482A
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Mobile phase for liquid chromatography
Assignee: Salk Institute for Biological Studies
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Filing: 1978-04-28
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Granted: 1983-03-22
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Expires: 1998-04-28
Abstract
Abstract A mobile phase for use in reversed phase high performance liquid chromatography. The mobile phase is a mixture of a polar organic solvent selected from acetonitrile and methanol and an aqueous solution of a salt of a tertiary amine and phosphoric acid or formic acid. The tertiary amines are selected from the group consisting of trialkyl amines and cyclic tertiary amines such as N-methyl morpholine, wherein the alkyl moiety is selected from methyl, ethyl, propyl, butyl and mixtures thereof.
Inventors
- 👤 Jean E. F. Rivier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:13
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/28; G01N30/34; B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/16; B01D15/166; B01D15/26; B01D15/32; B01D15/325; C; C07; C07K; C07K14/00; C07K14/435; C07K14/665; C07K14/675
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
71 words
A mobile phase for use in analysis of peptides by reversed-phase high performance liquid chromatography comprising a mixture of a polar organic solvent selected from the group consisting of acetonitrile and methanol and an aqueous solution of a tertiary amine selected from the group consisting of trialkylammonium phosphate, trialkylammonium formate and N-alkyl morpholine, wherein said alkyl moiety is selected from the group consisting of methyl, ethyl, propyl, butyl and mixtures thereof.
Claim 2
Dependent Claim
71 words
A mobile phase for use in analysis of peptides by reversed-phase high performance liquid chromatography comprising a mixture of a polar organic solvent selected from the group consisting of acetonitrile and methanol and an aqueous solution of a tertiary amine selected from the group consisting of trialkylammonium phosphate, trialkylammonium formate and N-alkyl morpholine, wherein said alkyl moiety is selected from the group consisting of methyl, ethyl, propyl, butyl and mixtures thereof.
Claim 3
Dependent Claim
25 words
A mobile phase in accordance with claim 1 wherein said polar solvent is acetonitrile and said tertiary amine salt of said solution is trialkylammonium phosphate.
Claim 4
Dependent Claim
15 words
A mobile phase in accordance with claim 2 wherein said trialkylammonium phosphate is triethylammonium phosphate.
Claim 5
Dependent Claim
41 words
A mobile phase in accordance with claim 1 wherein said phosphate or said formate moiety is present at a level of from about 0.05 to about 0.5 N and the pH of said solution is from about 2 to about 4.
Claim 6
Dependent Claim
25 words
A mobile phase in accordance with claim 4 wherein said polar solvent is acetonitrile and said tertiary amine salt of said solution is triethylammonium phosphate.
Claim 7
Independent Claim
95 words
A method for resolving mixtures of peptides by reversed-phase high performance liquid chromatography comprising providing a column packed with hydrocarbonaceous functional groups bonded to a carrier, introducing a peptide mixture into the end of said column and eluting said column with a mobile phase comprising a mixture of a polar solvent selected from the group consisting of acetonitrile and methanol and an aqueous solution of a tertiary amine selected from the group consisting of trialkylammonium phosphate, trialkylammonium formate and N-alkyl morpholine wherein said alkyl moiety is selected from methyl, ethyl, propyl, butyl and mixtures thereof.
Claim 8
Independent Claim
95 words
A method for resolving mixtures of peptides by reversed-phase high performance liquid chromatography comprising providing a column packed with hydrocarbonaceous functional groups bonded to a carrier, introducing a peptide mixture into the end of said column and eluting said column with a mobile phase comprising a mixture of a polar solvent selected from the group consisting of acetonitrile and methanol and an aqueous solution of a tertiary amine selected from the group consisting of trialkylammonium phosphate, trialkylammonium formate and N-alkyl morpholine wherein said alkyl moiety is selected from methyl, ethyl, propyl, butyl and mixtures thereof.
Claim 9
Dependent Claim
24 words
A method in accordance with claim 6 wherein said polar solvent is acetonitrile and said tertiary amine salt of said solution is trialkylammonium phosphate.
Claim 10
Dependent Claim
14 words
A method in accordance with claim 7 wherein said trialkylammonium phosphate is triethylammonium phosphate.
Claim 11
Dependent Claim
40 words
A method in accordance with claim 6 wherein said phosphate or said formate moiety is present at a level of from about 0.5 to about 0.5 N and the pH of said solution is from about 2 to about 4.
Claim 12
Dependent Claim
24 words
A method in accordance with claim 6 wherein said polar solvent is acetonitrile and said tertiary amine salt of said solution is triethylammonium phosphate.
Claim 13
Dependent Claim
52 words
A method in accordance with claim 9 wherein said acetonitrile is present at a level of from about 0 to about 85 percent, said phosphate moiety is present at a level of from about 0.1 to about 0.25 N and the pH of said solution is from about 2.5 to about 3.5.
Claim 14
Dependent Claim
49 words
A mobile phase in accordance with claim 1 wherein said polar organic solvent is present in said mixture at a level of from about 1 to about 85 percent and said tertiary amine is present in said mixture at a level of from about 15 to about 99 percent.
Claim 15
Dependent Claim
48 words
A method in accordance with claim 6 wherein said polar organic solvent is present in said mixture at a level of from about 1 to about 85 percent and said tertiary amine is present in said mixture at a level of from about 15 to about 99 percent.
References Cited (Backward Citations)
US3002823A
Cited
US4042327A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1986-03-21 | 1986-09-20 | 1987-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-03-21 | 1990-09-20 | 1991-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-03-21 | 1994-09-20 | 1995-03-22 | $7700.00 | Window Closed / Lapsed |