US4042327A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ion-pairing chromatography
Assignee: Waters Investments Ltd
•
Filing: 1976-01-15
•
Granted: 1977-08-16
•
Expires: 1996-01-15
Abstract
Abstract Liquid chromatographic processing of compositions containing ionizable compounds. The chromatographic column is formed of a stationary packing having a chemical group chemically bonded thereto and a counter ion is added to the polar mobile liquid. The counter ion forms a reversible complex with at least one characterizing radical of at least one of the ionizable compounds.
Inventors
- 👤 William G. Haney
- 👤 Douglas P. Wittmer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:29
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/287; B01J2220/00; B01J2220/50; B01J2220/54; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/145555; Y10T436/147777; Y10T436/17; Y10T436/173845; Y10T436/20; Y10T436/200833
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
141 words
In liquid chromatographic processing of compositions containing ionizable compounds, wherein the sample to be processed is carried in a mobile liquid phase which is passed through a porous packing material, in intimate physical contact therewith, and wherein said packing material has a chemical group chemically bonded onto the surface of said packing, the improvement comprising the steps of: 1. dissolving a counter ion, to a characterizing radical of at least one of said ionizable compounds, in a polar mobile liquid; 2. forming a reversible complex between said counter ion and at least one said characterizing radical, said complex being soluble in said polar liquid; 3. passing said complex through said packing for a time sufficient that chemical attraction between said complex and chemically-bonded group causes substantial retardation of the complex through the column; and 4. analyzing the effluent of said column.
Claim 2
Dependent Claim
141 words
In liquid chromatographic processing of compositions containing ionizable compounds, wherein the sample to be processed is carried in a mobile liquid phase which is passed through a porous packing material, in intimate physical contact therewith, and wherein said packing material has a chemical group chemically bonded onto the surface of said packing, the improvement comprising the steps of: 1. dissolving a counter ion, to a characterizing radical of at least one of said ionizable compounds, in a polar mobile liquid; 2. forming a reversible complex between said counter ion and at least one said characterizing radical, said complex being soluble in said polar liquid; 3. passing said complex through said packing for a time sufficient that chemical attraction between said complex and chemically-bonded group causes substantial retardation of the complex through the column; and 4. analyzing the effluent of said column.
Claim 3
Dependent Claim
28 words
A process as defined in claim 1 wherein a said complex is formed between a strong acid bearing a counter-ion and a strong base bearing a characterizing radical.
Claim 4
Dependent Claim
28 words
A process as defined in claim 1 wherein a said complex is formed between a strong base bearing a counter-ion and a strong acid bearing a characterizing radical.
Claim 5
Dependent Claim
37 words
A process as defined in claim 1 wherein said composition containing ionizable compounds comprises both acids and bases bearing characterizing radicals, and said analyzing step includes analysis of at least one acid and at least one base.
Claim 6
Dependent Claim
34 words
A process as defined in claim 1 wherein a said complex is formed between a characterizing radical of highly-water-soluble weak acid or a highly-water-soluble weak base and a counter ion for said characterizing radical.
Claim 7
Dependent Claim
22 words
A process as defined in claim 5 wherein said complex is formed between an ascorbic characterizing radical and an aliphatic counter ion.
Claim 8
Dependent Claim
25 words
A process as defined in claim 1 wherein some characterizing radical is derived from a sulfonic acid compound in the composition being subjected to analysis.
Claim 9
Dependent Claim
25 words
A process as defined in claim 1 wherein said characterizing radical is derived from a quarternary amine compound in the composition being subjected to analysis.
Claim 10
Dependent Claim
20 words
A process as defined in claim 1 wherein said chemical group bonded to the surface of said packing is aliphatic.
Claim 11
Dependent Claim
20 words
A process as defined in claim 1 wherein said chemical group bonded to said packing has a strong hydrogen-bonding capacity.
Claim 12
Dependent Claim
15 words
A process as defined in claim 10 wherein said chemical group bears a --CN radical.
Claim 13
Dependent Claim
50 words
A process as defined in claim 1 wherein the relative sequential position of at least one characterizing radical, in a column effluent comprising a number of chemical compounds, is shifted by formation of a said complex formed between a said characterizing radical and a counter ion for said characterizing radical.
Claim 14
Dependent Claim
22 words
A process as defined in claim 1 where at least one said characterizing radical is obtained from ionization of an amphoteric compound.
Claim 15
Dependent Claim
60 words
A process as defined in claim 1 wherein at least one said ionizable compound to be subjected to analysis is chemically unstable, in the form in which it is introduced into the column in the absence of a said counter ion, but which forms a complex with a said counter ion, thereby providing means to analyze for said ionizable compound.
Claim 16
Dependent Claim
15 words
A process as defined in claim 3 wherein said base is a quaternary ammonium compound.
Claim 17
Dependent Claim
14 words
A process as defined in claim 2 wherein said acid is a sulfonic acid.
Claim 18
Dependent Claim
18 words
A process as defined in claim 14 wherein said compound to be analyzed is unstable in non-ionized form.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-15 | 1981-02-14 | 1981-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-15 | 1985-02-14 | 1985-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-15 | 1989-02-14 | 1989-08-16 | $7700.00 | Window Closed / Lapsed |