Patent US4376689A successfully refreshed from Google Patents.
US4376689A
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Coenzyme immobilized electrode
Assignee: Panasonic Holdings Corp
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Filing: 1981-11-13
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Granted: 1983-03-15
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Expires: 2001-11-13
Abstract
Abstract Improvements in a coenzyme electrode used to electrochemically measure the activity of enzyme or substrate concentration of the enzyme easily. The coenzyme is immobilized, without using a semipermeable membrane, on the electron collector directly with the chemical bond, particularly the covalent bond, whereby the activity of the oxide-reductase requiring the coenzyme can be measured. In addition, the oxide-reductase requiring the coenzyme is also immobilized together with such immobilized coenzyme to improve the characteristics of the conventional enzyme-coenzyme immobilized electrode.
Inventors
- 👤 Kenichi Nakamura
- 👤 Shiro Nankai
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 22:30
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
70 words
A process for preparing a coenzyme immobilized electrode, which comprises (1) providing a carrier adapted to immobilize, through covalent bonding, a coenzyme of oxidoreductase, said coenzyme being selected from the group consisting of NAD and NADP, (2) immobilizing said coenzyme on said carrier, (3) mixing said carrier, having said coenzyme immobilized thereon through covalent bonding, with an electron collector, and (4) press-molding the resultant mixture to form a coenzyme electrode.
Claim 2
Dependent Claim
70 words
A process for preparing a coenzyme immobilized electrode, which comprises (1) providing a carrier adapted to immobilize, through covalent bonding, a coenzyme of oxidoreductase, said coenzyme being selected from the group consisting of NAD and NADP, (2) immobilizing said coenzyme on said carrier, (3) mixing said carrier, having said coenzyme immobilized thereon through covalent bonding, with an electron collector, and (4) press-molding the resultant mixture to form a coenzyme electrode.
Claim 3
Dependent Claim
56 words
A coenzyme immobilized electrode comprising at least a coenzyme of oxidoreductase, an electron collector and a carrier, said coenzyme, which is selected from the group consisting of NAD and NADP, being immobilized through covalent bonding on said carrier which is mixed with said electron collector, said immobilized coenzyme being in electrical contact with said electron collector.
Claim 4
Dependent Claim
56 words
A coenzyme immobilized electrode comprising at least a coenzyme of oxidoreductase, an electron collector and a carrier, said coenzyme, which is selected from the group consisting of NAD and NADP, being immobilized through covalent bonding on said carrier which is mixed with said electron collector, said immobilized coenzyme being in electrical contact with said electron collector.
Claim 5
Dependent Claim
29 words
A coenzyme immobilized electrode in accordance with claim 2, wherein said carrier is selected from the group consisting of albumin, agarose, cellulose, silica glass, alumina, silica alumina and zeolite.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-03-14 | 1986-09-13 | 1987-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-03-14 | 1990-09-13 | 1991-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-03-14 | 1994-09-13 | 1995-03-15 | $7700.00 | Window Closed / Lapsed |