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US4374644A
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Blood cell volume monitoring
Assignee: Coulter Electronics Inc
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Filing: 1981-04-06
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Granted: 1983-02-22
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Expires: 2001-04-06
Abstract
Abstract By subjecting cells, such as red blood cells, to a hypotonic solution or a solution having a lytic agent, the cells will rapidly attain a change in volume and electrical resistance parameters, which change is measured as a function of time, for example, by a Coulter Counter® particle measuring instrument. The relationship between the change in the cell resistance or cell volume and the time of immersion in the volume changing solution provides a discriminator between a normality, different diseases, and certain inherited differences.
Inventors
- 👤 Douglas Armstrong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:28
CPC Classifications
G; G01; G01N; G01N13/00; G01N13/04; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
146 words
A method of testing cells comprising the steps of: forming a mixture of at least one portion of a cell sample with a cell volume increasing solution having a predetermined, substantially unchanging composition, the cells of said cell sample each having an original volume; causing the cells to reside in said mixture for at least a predetermined duration during which the cells attain a change increase in their volume as compared to their original volume, the amount of said change depending upon the time of immersion of the cells in said cell volume increasing solution and the innate and acquired properties of each cell; measuring the change of volume of the cells as a function of time; and employing said measured differences in cell volume to define data which is capable of being compared with cell volume data representative of a known health or physiological condition.
Claim 2
Independent Claim
146 words
A method of testing cells comprising the steps of: forming a mixture of at least one portion of a cell sample with a cell volume increasing solution having a predetermined, substantially unchanging composition, the cells of said cell sample each having an original volume; causing the cells to reside in said mixture for at least a predetermined duration during which the cells attain a change increase in their volume as compared to their original volume, the amount of said change depending upon the time of immersion of the cells in said cell volume increasing solution and the innate and acquired properties of each cell; measuring the change of volume of the cells as a function of time; and employing said measured differences in cell volume to define data which is capable of being compared with cell volume data representative of a known health or physiological condition.
Claim 3
Dependent Claim
15 words
The method according to claim 1, wherein said volume changing solution comprises a hypotonic solution.
Claim 4
Dependent Claim
18 words
The method according to claim 1, wherein said volume changing solution comprises a solution having a hemolytic agent.
Claim 5
Dependent Claim
18 words
The method according to claim 1, wherein said step of measuring obtains the apparent volumes of the cells.
Claim 6
Dependent Claim
20 words
The method according to claim 1, wherein said step of measuring obtains the apparent mean cell volume of the cells.
Claim 7
Dependent Claim
27 words
The method according to claim 1 or 5, wherein said step of measuring obtains the change in electrical resistance of the cells as a function of time.
Claim 8
Dependent Claim
27 words
The method according to claim 1 or 5, wherein said step of measuring obtains the change in optical signals of the cells as a function of time.
Claim 9
Dependent Claim
34 words
The method according to claim 1, wherein such step of measuring includes correlating a given attained mean cell volume measurement with the specific time of occurance that provides said given mean cell volume measurement.
Claim 10
Dependent Claim
43 words
The method according to claim 1, wherein said step of employing includes correlating a given mean cell volume measurement with an earlier mean cell volume measurement so as to derive the percent of change relative to the said earlier mean cell volume measurement.
Claim 11
Dependent Claim
22 words
The method according to claim 1, wherein said step of employing includes forming a curve plotted from said defined set of data.
Claim 12
Dependent Claim
17 words
The method of claim 1, wherein the step of measuring comprises monitoring the mean cell volume continuously.
Claim 13
Dependent Claim
26 words
The method according to claim 1, wherein the step of measuring comprises making repeated mean cell volume measurements on the blood sample at appropriate time intervals.
Claim 14
Dependent Claim
53 words
The method of claim 1, wherein said predetermined duration is of sufficient length to allow lysing of the white cells, the method including the further step of, after measuring the change in electrical resistance of red blood cells, measuring the change in electrical resistance of white blood cells during the same operational run.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-02-21 | 1986-08-23 | 1987-02-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-02-21 | 1990-08-23 | 1991-02-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-02-21 | 1994-08-23 | 1995-02-22 | $7700.00 | Window Closed / Lapsed |