Apparatus and method for measuring osmotic fragility of red blood cells
Abstract
Abstract MEASURING OSMOTIC FRAGILITY OF ERYTHROCYTES FOR DIAGNOSIS OF BLOOD DYSCRASIAS. A BLOOD SAMPLE, DILUTED IN AN ISOTONIC SALINE SOLUTION, IS PLACED IN A TEST TUBE WHICH IN TURN, IS POSITIONED IN APPARATUS FOR MEASURING OSMOTIC FRAGILITY OF ERYTHOCYTES IN THE SAMPLE. IN THE APPARATUS, DISTILLED WATER IS DELIVERED DIRECTLY INTO THE SAMPLE AT A PREDETERMINED CONSTANT RATE WITH CONTINUOUS MIXING TO MAINTAIN EQUILIBRIUM OF TONICITY THROUGHOUT THE SAMPLE. THE SAMPLE IS TRANSILLUMINATED DURING SUCH DILUTION AND LIGHT TRANSMITTANCE THROUGH THE SAMPLE IS PHOTOELECTRICALLY MEASURED AND RECORDED TO INDICATE THE RATE AND PERCENT OF HEMOLYSIS VERSUS TIME OR TONICITY. TONICITIES OF THE SAMPLE AT WHICH HEMOLYSIS STARTS, ENDS AND/OR REACHES OTHER VALUES ARE DETERMINED FROM THE RECORDING FOR INTERPRETATION OF ERYTHROCYTIC OSMOTIC FRAGILITY.
Inventors
- 👤 Michael L Polanyi
- 👤 Henry Lustig
Bibliographic Details
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