Patent US4367072A successfully refreshed from Google Patents.
US4367072A
Expired / Lapsed
US
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Ligands assayed by host molecules including cyclophanes, crown ethers, crypstands and podands
Assignee: Roche Diagnostics GmbH
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Filing: 1979-09-25
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Granted: 1983-01-04
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Expires: 1999-09-25
Abstract
Abstract Method for the determination of a component in a liquid which component is selected from ions, polar substances lipophilic substances, which comprises contacting a liquid sample containing said component with, and allowing same to act upon, a complex ligand or host molecule which is selective toward the component, said complex ligand or host molecule being bonded to a chromophore material, and measuring the change of extinction or the wavelength displacement of the resulting mixture as a measure of the initial content of said component; corresponding reagent test kits are also provided. The complex ligands or host molecules include cyclodextrins, cyclophanes, crown ethers, cryptands, podands valinomycin, gramacidin and monactin.
Inventors
- 👤 Friedrich Vogtle
- 👤 Johannes P. Dix
- 👤 Dieter Jaworek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 06:06
CPC Classifications
C; C10; C10M; C10M161/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/84; G01N2800/00; G01N2800/04; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/808; Y10S436/815
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
121 words
Method for the determination of a component in a liquid which component is selected from ions, polar substances and lipophilic substances, which method comprises contacting a liquid sample containing said component with, and allowing same to act upon, a complex ligand or hose molecule which is selective toward the component, and being selected from the group consisting of a cyclodextrin, a cyclophane, a crown ether or derivative thereof, a cryptand or derivative thereof, a podand or derivative thereof, a valinomycin, a gramacidin and a nonactin said complex ligand or host molecule being bonded to a chromophore material, and measuring the change of extinction or the wavelength displacement of the resulting mixture as a measure of the initial content of said component.
Claim 2
Dependent Claim
121 words
Method for the determination of a component in a liquid which component is selected from ions, polar substances and lipophilic substances, which method comprises contacting a liquid sample containing said component with, and allowing same to act upon, a complex ligand or hose molecule which is selective toward the component, and being selected from the group consisting of a cyclodextrin, a cyclophane, a crown ether or derivative thereof, a cryptand or derivative thereof, a podand or derivative thereof, a valinomycin, a gramacidin and a nonactin said complex ligand or host molecule being bonded to a chromophore material, and measuring the change of extinction or the wavelength displacement of the resulting mixture as a measure of the initial content of said component.
Claim 3
Dependent Claim
13 words
Method as claimed in claim 1 wherein said component is a polar substance.
Claim 4
Dependent Claim
13 words
Method as claimed in claim 1 wherein said component is a lipophilic substance.
Claim 5
Dependent Claim
17 words
Method as claimed in claim 1 wherein said component is a mixture of polar and lipophilic substances.
Claim 6
Dependent Claim
18 words
Method as claimed in claim 1 wherein said chromophore material is in the form of an inclusion complex.
Claim 7
Dependent Claim
13 words
Method as claimed in claim 1 wherein said host molecule is a cyclodextrin.
Claim 8
Dependent Claim
13 words
Method as claimed in claim 1 wherein said host molecule is a cyclophane.
Claim 9
Dependent Claim
18 words
Method as claimed in claim 1 wherein said selective complex ligand is a crown ether or derivative thereof.
Claim 10
Dependent Claim
17 words
Method as claimed in claim 1 wherein said selective complex ligand is a cryptand or derivative thereof.
Claim 11
Dependent Claim
17 words
Method as claimed in claim 1 wherein said selective complex ligand is a podand or derivative thereof.
Claim 12
Dependent Claim
16 words
Method as claimed in claim 1 wherein said component is constituted by ions in said liquid.
Claim 13
Dependent Claim
12 words
Method as claimed in claim 11 wherein said component is an anion.
Claim 14
Dependent Claim
26 words
Method as claimed in claim 12 wherein said anion is a chloride, bromide, iodide, sulfate, nitrate, nitrite, phosphate, diphosphate, triphosphate, hydrogen phosphate or hydrogen carbonate ion.
Claim 15
Dependent Claim
12 words
Method as claimed in claim 11 wherein said component is an cation.
Claim 16
Dependent Claim
21 words
Method as claimed in claim 14 wherein said cation is an ammonium, alkali metal, alkaline earth metal or other metal ion.
Claim 17
Dependent Claim
29 words
Method as claimed in claim 15 wherein said cation is a lithium, sodium, potassium, magnesium or calcium ion or an iron, zinc, copper, cobalt, nickel, molybdenum or chromium ion.
Claim 18
Dependent Claim
18 words
Method as claimed in claim 1 wherein said complex ligand is a valinomycin, a gramacidin or a nonactin.
Claim 19
Dependent Claim
30 words
Method as claimed in claim 1 wherein said component is a polar substance selected from the group consisting of urea, thiourea, guanidine, uric acid, choline, creatinine, amino acids and sugars.
Claim 20
Dependent Claim
21 words
Method as claimed in claim 1 wherein said chromophore is linked to several identical or different complex ligands or host molecules.
Claim 21
Dependent Claim
21 words
Method as claimed in claim 1 wherein said complex ligand or host molecule is linked to several identical or different chromophores.
Claim 22
Dependent Claim
23 words
Method as claimed in claim 1 wherein said complex ligand or host molecule is bonded to the chromophore via a direct covalent bond.
Claim 23
Dependent Claim
22 words
Method as claimed in claim 1 wherein said complex ligand or host molecule is bonded to the chromophore via a heteropolar bond.
Claim 24
Dependent Claim
22 words
Method as claimed in claim 1 wherein said complex ligand or host molecule is bonded to the chromophore via a hydrophobic bond.
Claim 25
Dependent Claim
22 words
Method as claimed in claim 1 wherein said complex ligand or host molecule is bonded to the chromophore via a hydrogen bridge.
Claim 26
Dependent Claim
22 words
Method as claimed in claim 1 wherein said component is a lipophilic substance selected from the group consisting of steroids and lipids.
Claim 27
Dependent Claim
15 words
Method as claimed in claim 25 wherein said lipophilic substance is cholesterol, triglyceride or lecithin.
Claim 28
Dependent Claim
22 words
Method as claimed in claim 1 wherein said complex ligand or host molecule is applied to or incorporated into a solid carrier.
Claim 29
Dependent Claim
25 words
Method as claimed in claim 27 wherein said carrier is paper, synthetic resin film, glass, aluminum oxide, silicon oxide, natural or synthetic fibers or metal.
Claim 30
Dependent Claim
37 words
Method as claimed in claim 1 wherein said chromophore is a dyestuff or a chromogen having an absorption spectrum which is changed by reciprocal action with said component, by charge displacement or disturbance of the mesomeric system.
Claim 31
Dependent Claim
33 words
Method as claimed in claim 29 wherein said chromophore is selected from materials having a polyene, meriquinoid, quinone, azo, pyrrole, merocyanin, indigo, indophenol, stilbene, azomethine, anthraquinone, naphthoquinone, cyanine, phthalein, polymethine or alizarine structure.
Claim 32
Dependent Claim
17 words
Method as claimed in claim 29 wherein said dyestuff contains a carboxylate, sulfonate, phenolate or thiophenolate grouping.
Claim 33
Dependent Claim
14 words
Method as claimed in claim 29 wherein said chromophore is an acidic dyestuff thereof.
Claim 34
Dependent Claim
27 words
Method as claimed in claim 32 wherein said chromophore is a salt of an acidic dyestuff selected from lithium, sodium, potassium, ammonium, calcium, alkylammonium or magnesium salts.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-03 | 1986-07-05 | 1987-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-03 | 1990-07-05 | 1991-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-03 | 1994-07-05 | 1995-01-04 | $7700.00 | Window Closed / Lapsed |