Patent US4335208A successfully refreshed from Google Patents.
US4335208A
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US
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Saccharification of starch hydrolysates
Assignee: Novo Nordisk AS
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Filing: 1980-03-11
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Granted: 1982-06-15
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Expires: 2000-03-11
Abstract
Abstract Saccharifying starch hydrolysate to a high DX glucose syrup at pH 3-5 by the enzyme mixture of a glucoamylase and an acidophilic iso-amylase. A lower dosage level than the heretofore conventional glucoamylase dosage may be employed and higher DX glucose syrups can be obtained.
Inventors
- 👤 Barrie E. Norman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:04
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/16; C07; C07H; C07H3/00; C07H3/02; C12P19/20; C13; C13K; C13K1/00; C13K1/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/911; Y10S435/913; Y10S435/917
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
72 words
In a process for saccharifying a starch hydrolysate to a high DX glucose syrup with glucoamylase, the improvement which comprises saccharifying at pH 3-5 in the presence of an enzyme mixture comprising effective amounts of glucoamylase and an acidophilic isoamylase to convert the starch hydrolysate to at least about 96 DX glucose syrup, the glucoamylase dosage being in the range of 0.05-0.15 AG units per gram of dry substance in the hydrolysate.
Claim 2
Dependent Claim
72 words
In a process for saccharifying a starch hydrolysate to a high DX glucose syrup with glucoamylase, the improvement which comprises saccharifying at pH 3-5 in the presence of an enzyme mixture comprising effective amounts of glucoamylase and an acidophilic isoamylase to convert the starch hydrolysate to at least about 96 DX glucose syrup, the glucoamylase dosage being in the range of 0.05-0.15 AG units per gram of dry substance in the hydrolysate.
Claim 3
Dependent Claim
18 words
The process of claim 1 wherein the solids content in the starch hydrolysate exceeds about 30% by weight.
Claim 4
Dependent Claim
17 words
The process of claim 1 wherein the saccharifying temperature is in the range of 50° C.-60° C.
Claim 5
Dependent Claim
15 words
The process of claim 1 wherein the saccharifying reaction is carried out for 24-96 hours.
Claim 6
Dependent Claim
25 words
The process of claim 1 wherein the isoamylase dosage is in the range of 25-500 IA units per gram of dry substance in the hydrolysate.
Claim 7
Dependent Claim
39 words
The process of claim 1 wherein the glucoamylase dosage is in the range of 0.075-0.15 AG units and the isoamylase dosage is in the range of 100-200 IA units, each being per gram of dry substance in the hydrolysate.
Claim 8
Dependent Claim
12 words
The process of claim 1 wherein the isoamylase is from Pseudomonas amyloderamosa.
Claim 9
Dependent Claim
12 words
The process of claim 7 wherein the glucoamylase is from Aspergillus niger.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-14 | 1985-12-14 | 1986-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-14 | 1989-12-14 | 1990-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-14 | 1993-12-14 | 1994-06-15 | $7700.00 | Window Closed / Lapsed |