US3897305A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing dextrose
Assignee: Primary Products Ingredients Americas LLC
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Filing: 1973-12-17
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Granted: 1975-07-29
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Expires: 1993-12-17
Abstract
Abstract A method for converting starch to dextrose by saccharifying a starch hydrolyzate with an enzyme system comprising glucoamylase and amylo-1,6-glucosidase at a pH which will inhibit the reversionary action of glucoamylase. Preferably the starch hydrolyzate is obtained by thinning starch with an enzyme such as alpha-amylase and is saccharified with the above enzyme system at a pH of between 4.5 and 6.7.
Inventors
- 👤 Thomas L Hurst
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:51
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/20; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/828; Y10S435/911; Y10S435/913; Y10S435/939
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
87 words
IN A PROCESS FOR PRODUCING A CONVERSION SYRUP OF A HIGH DEXTROSE CONTENT BY SACCHARIFYING UNDER ACID CONDITIONS, A THINNED STARCH HYDROLYZATE TO DEXTROSE WITH GLUCOAMYLASE, THE IMPROVEMENT WHICH COMPRISES SACCHARIFYING THE THINNED HYDROLUZATE TO DEXTROSE AT PH BETWEEN 5.0 AND 6.7 IN THE PRESENCE OF AN ENZYME SYSTEM COMPRISING GLUCOAMYLASE AND AN EFFECTIVE AMOUNT OF AMYLO-1,6-GLUCOSIDASE TO HYDROLYZE 1,6GLUCOSIDIC LINKAGES OF THE HYDROLYZATE WITH THE SACCHARIFICATION BEING CONDUCTED AT A PH SUFFICIENT TO INHIBIT THE REVERSIONARY ACTION OF GLUCOAMYLASE AND THEREBY OBTAIN IMPROVED YIELDS OF DEXTROSE.
Claim 2
Dependent Claim
87 words
IN A PROCESS FOR PRODUCING A CONVERSION SYRUP OF A HIGH DEXTROSE CONTENT BY SACCHARIFYING UNDER ACID CONDITIONS, A THINNED STARCH HYDROLYZATE TO DEXTROSE WITH GLUCOAMYLASE, THE IMPROVEMENT WHICH COMPRISES SACCHARIFYING THE THINNED HYDROLUZATE TO DEXTROSE AT PH BETWEEN 5.0 AND 6.7 IN THE PRESENCE OF AN ENZYME SYSTEM COMPRISING GLUCOAMYLASE AND AN EFFECTIVE AMOUNT OF AMYLO-1,6-GLUCOSIDASE TO HYDROLYZE 1,6GLUCOSIDIC LINKAGES OF THE HYDROLYZATE WITH THE SACCHARIFICATION BEING CONDUCTED AT A PH SUFFICIENT TO INHIBIT THE REVERSIONARY ACTION OF GLUCOAMYLASE AND THEREBY OBTAIN IMPROVED YIELDS OF DEXTROSE.
Claim 3
Dependent Claim
22 words
The process according to claim 1 wherein the thinned starch hydrolyzate has a dry solids content of 20% to 55% by weight.
Claim 4
Dependent Claim
27 words
The process according to claim 2 wherein the thinned hydrolyzate is saccharified in the presence of at least 0.5 units of pullulanase per gram of starch solids.
Claim 5
Dependent Claim
32 words
The process according to claim 1 wherein the glucoamylase is substantially free from transglucosidase and is obtained from at least one microorganism selected froM the genus of Aspergillus, Clostridium, Mucor and Rhizopus.
Claim 6
Dependent Claim
31 words
The process according to claim 4 wherein the thinned starch hydrolyzate has a dry solids content from 30% to 50% by weight and is saccharified to dextrose with an Aspergillus glucoamylase.
Claim 7
Dependent Claim
55 words
The process according to claim 2 wherein the saccharification of the thinned starch hydrolyzate to dextrose is conducted in the presence of an enzyme system substantially free from transglucosidase and the enzyme system consisting essentially of glucoamylase and pullulanase at a potency ratio of 1.5 to 4.0 units of glucoamylase for each unit of pullulanase.
Claim 8
Dependent Claim
19 words
The process according to claim 6 wherein the thinned starch hydrolyzate is saccharified to dextrose with an Aspergillus glucoamylase.
Claim 9
Dependent Claim
20 words
The process according to claim 7 wherein the thinned starch hydrolyzate contains at least about 30% by weight dry solids.
Claim 10
Dependent Claim
49 words
The process according to claim 2 wherein the saccharification is conducted at a pH of at least 5.5 in the presence of amylo-1, 6-glucosidase and glucoamylase at a potency ratio of 0.05 to 0.8 units amylo-1,6-glucosidase per unit of glucoamylase and the enzyme system is substantially free from transglucosidase.
Claim 11
Dependent Claim
23 words
The process according to claim 2 wherein the saccharification of the thinned starch hydrolyzate is conducted at a pH of 5.5 to 6.3.
Claim 12
Dependent Claim
29 words
The process according to claim 10 wherein the enzyme system has a potency ratio of about 1.5 units to about 4.0 units of glucoamylase for each unit of amylo-1,6-glucosidase.
Claim 13
Dependent Claim
29 words
The process according to claim 10 wherein the enzyme system comprises Rhizopus glucoamylase and pullulanase and the thinned starch hydrolyzate contains at least about 30% by weight dry solids.
Claim 14
Dependent Claim
35 words
The process according to claim 11 wherein the thinned hydrolyzate is saccharified to dextrose with Aspergillus glucoamylase in the presence of pullulanase and the thinned starch hydrolyzate contains at least 30% by weight dry solids.
Claim 15
Dependent Claim
70 words
The process according to claim 10 wherein the thinned starch hydrolyzate contains about 30% to 40% by weight dry solids and the enzyme system comprises from 0.5 to 6.0 units of pullulanase per gram of dry starch solids and from 4 to 15 units of glucoamylase per gram of dry starch solids and the glucoamylase is derived from at least one microorganism selected from the genus of Aspergillus and Rhizopus.
Claim 16
Dependent Claim
19 words
The process according to claim 14 wherein the thinned starch hydrolyzate is saccharified to dextrose with an Aspergillus glucoamylase.
Claim 17
Dependent Claim
12 words
The process according to claim 2 wherein amylo-1,6-glusoidase consists essentially of isoamylase.
Claim 18
Dependent Claim
23 words
The process of claim 10 wherein the enzyme system also contains alpha-amylase in an amount sufficient to inhibit retrogradation of the thinned starch.
Claim 19
Dependent Claim
33 words
A process for producing dextrose comprising saccharifying a thinned starch hydrolyzate to dextrose with an enzyme system comprising glucoamylase and an effective amount of amylo-1,6-glucosidase at a pH between about 5.8 and 6.3.
Claim 20
Dependent Claim
33 words
A process for producing dextrose comprising saccharifying a thinned starch hydrolyzate to dextrose with an enzyme system comprising glucoamylase and an effective amount of amylo-1,6-glucosidase at a pH between about 5.8 and 6.3.
Claim 21
Dependent Claim
28 words
The process of claim 17 wherein the enzymes are present in said enzyme system in a potency ratio equivalent to 0.05-0.8 units of amylo-1,6-glucosidase per unit of glucoamylase.
Claim 22
Dependent Claim
28 words
The process of claim 18 wherein the enzymes are present in said enzyme system in a potency ratio equivalent to 1.5-4.0 units of glucoamylase per unit of amylo-1,6-glucosidase.
References Cited (Backward Citations)
US2891869A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-07-28 | 1979-01-27 | 1979-07-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-07-28 | 1983-01-27 | 1983-07-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-07-28 | 1987-01-27 | 1987-07-29 | $7700.00 | Window Closed / Lapsed |