Patent US4329151A successfully refreshed from Google Patents.
US4329151A
Expired / Lapsed
US
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Stable diagnostic reagent and method for qualitative determinations of streptococci infections
Assignee: ICL SCIENTIFIC Inc
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Filing: 1979-08-17
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Granted: 1982-05-11
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Expires: 1999-08-17
Abstract
Abstract A method and a stable reagent for the detection of pathogenic bacterial infections in humans, and in particular, a stable diagnostic composition comprising non-degraded streptolysin-O protein directly adsorbed onto polystyrene latex particles and method of use are disclosed. The reagent may be prepared by adsorbing one or more layers of the streptolysin-O toxin onto the surface of the latex particle, effecting an intermolecular cross-linkage of the streptolysin-O, and further stabilizing the reagent by the addition of a bacteriostatic agent. Accordingly, the present invention provides a significant advance regarding the qualitative determinations of streptococci infections by providing a stable diagnostic reagent and method for such determinations.
Inventors
- 👤 Kingdon Lou
- 👤 Tracey L. Burton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; G01N33/569; G01N33/56911; G01N33/56944; Y; Y10; Y10S; Y10S436/00; Y10S436/826
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
75 words
A stable reagent for the chemical diagnosis of pathogenic bacterial infections in humans and, in particular, for the qualitative determination of streptococci infections in human serum, the reagent comprising a substantially uniform polystyrene latex particle, said particle being directly adsorbed at a pH between about 8.5 and 10.9 with a cross-linked streptolysin-O protein coating and stabilized with a bacteriostatic agent, said coating being characterised in that it is substantially free from adsorption facilitating extraneous macromolecules.
Claim 2
Dependent Claim
75 words
A stable reagent for the chemical diagnosis of pathogenic bacterial infections in humans and, in particular, for the qualitative determination of streptococci infections in human serum, the reagent comprising a substantially uniform polystyrene latex particle, said particle being directly adsorbed at a pH between about 8.5 and 10.9 with a cross-linked streptolysin-O protein coating and stabilized with a bacteriostatic agent, said coating being characterised in that it is substantially free from adsorption facilitating extraneous macromolecules.
Claim 3
Dependent Claim
25 words
The reagent as defined in claim 1, wherein the average diameter of said polystyrene latex particle is from about 0.5 microns to about 0.7 microns.
Claim 4
Dependent Claim
24 words
The reagent as defined in claim 1, wherein said cross-linked streptolysin-O protein coating is formed between functional groups capable of reacting with water-soluble carbodiimide.
Claim 5
Dependent Claim
26 words
The reagent as defined in claim 1, wherein said bacteriostatic agent is sodium azide having concentration range of from about 0.05% (w/v) to about 2.0% (w/v).
Claim 6
Dependent Claim
21 words
The reagent as defined in claim 1, wherein said bacteriostatic agent is sodium azide having a concentration of about 0.10% (w/v).
Claim 7
Dependent Claim
26 words
The reagent as defined in claim 1, wherein said bacteriostatic agent is thimerosal having a concentration range of from about 0.00001% (w/v) to about 0.001% (w/v).
Claim 8
Dependent Claim
14 words
The reagent as defined in claim 1, said coating further including a biological stain.
Claim 9
Dependent Claim
14 words
The reagent as defined in claim 7, wherein said biological stain is Malachite green.
Claim 10
Independent Claim
99 words
A method for producing a stable diagnostic reagent having a coating of non-degraded streptolysin-O protein directly adsorbed onto a polystyrene latex particle, the method comprising the addition of the polystyrene latex particle, streptolysin-O protein and a water soluble carbodiimide to a buffered solution having a pH of from about pH 8.5 to about pH 11.9, stirring the resultant mixture for a sufficient period of time to form a sensitized particle having a non-degraded streptolysin-O protein coating, washing this sensitized particle and stabilizing said streptolysin-O coating by re-suspension of the sensitized particle in a buffered solution of a bacteriostatic agent.
Claim 11
Independent Claim
99 words
A method for producing a stable diagnostic reagent having a coating of non-degraded streptolysin-O protein directly adsorbed onto a polystyrene latex particle, the method comprising the addition of the polystyrene latex particle, streptolysin-O protein and a water soluble carbodiimide to a buffered solution having a pH of from about pH 8.5 to about pH 11.9, stirring the resultant mixture for a sufficient period of time to form a sensitized particle having a non-degraded streptolysin-O protein coating, washing this sensitized particle and stabilizing said streptolysin-O coating by re-suspension of the sensitized particle in a buffered solution of a bacteriostatic agent.
Claim 12
Dependent Claim
15 words
The method in claim 9, wherein said buffered solution is formed from an inorganic buffer.
Claim 13
Dependent Claim
16 words
The method in claim 9 wherein said buffered solution has a pH of about pH 10.
Claim 14
Dependent Claim
11 words
The method in claim 9 wherein said buffer is Boric Acid-KCl-NaOH.
Claim 15
Dependent Claim
21 words
The method in claim 9 wherein said carbodiimide is selected from the group consisting of 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide morpho-p-toluenesulfonate and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride.
Claim 16
Dependent Claim
12 words
The method in claim 9 wherein said bacteriostatic agent is sodium azide.
Claim 17
Dependent Claim
24 words
The method in claim 14 wherein said sodium azide is present in a concentration range of from about 0.05% (w/v) to about 2.0% (w/v).
Claim 18
Dependent Claim
11 words
The method in claim 9 wherein said bacteriostatic agent is thimerosal.
Claim 19
Dependent Claim
24 words
The method in claim 16 wherein said said thimerosal is present in a concentration range of from about 0.00001% (w/v) to about 0.001% (w/v).
Claim 20
Dependent Claim
18 words
The method in claim 9 and further including the addition of a biological stain to said buffered solution.
Claim 21
Dependent Claim
12 words
The method in claim 18 wherein said biological stain is Malachite green.
Claim 22
Independent Claim
157 words
A method for the chemical diagnosis of pathogenic bacterial infections in humans, and, in particular, for the qualitative determination of streptococci infections in human serum, the method comprising the steps of: providing a stable reagent comprising a substantially uniform polystyrene latex particle, said particle being directly adsorbed at a pH between about 8.5 and 11.9 with a cross-linked streptolysin-O protein coating and stabilized with a bacteriostatic agent, said coating being characterized in that it is substantially free from adsorption facilitating extraneous macromolecules; standardizing a solution of said streptolysin-O-polystyrene latex reagent to allow agglutination to occur when one drop of the solution is added to a 1:20 dilution of human serum containing antistreptolysn-O; diluting one part of the human serum to be tested with 19 parts of normal saline; mixing one drop of streptolysin-O-polystyrene latex reagent solution with one drop of human test serum on a suitable substrate, and visually determining the occurrence of a macroscopic agglutination product.
Claim 23
Independent Claim
157 words
A method for the chemical diagnosis of pathogenic bacterial infections in humans, and, in particular, for the qualitative determination of streptococci infections in human serum, the method comprising the steps of: providing a stable reagent comprising a substantially uniform polystyrene latex particle, said particle being directly adsorbed at a pH between about 8.5 and 11.9 with a cross-linked streptolysin-O protein coating and stabilized with a bacteriostatic agent, said coating being characterized in that it is substantially free from adsorption facilitating extraneous macromolecules; standardizing a solution of said streptolysin-O-polystyrene latex reagent to allow agglutination to occur when one drop of the solution is added to a 1:20 dilution of human serum containing antistreptolysn-O; diluting one part of the human serum to be tested with 19 parts of normal saline; mixing one drop of streptolysin-O-polystyrene latex reagent solution with one drop of human test serum on a suitable substrate, and visually determining the occurrence of a macroscopic agglutination product.
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