US3985867A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassays employing a colored second antibody
Assignee: GE Healthcare Ltd
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Filing: 1974-12-26
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Granted: 1976-10-12
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Expires: 1994-12-26
Abstract
Abstract In some radioimmunoassays, the antigen competes with labelled antigen for reaction with a first antibody and the antigen-antibody complex is precipitated by reaction with an aqueous solution of a second antibody to the first antibody. The invention consists in using an artificially colored second antibody, so that the precipitate is easily visible to the naked eye.
Inventors
- 👤 Martin Ralphs Redshaw
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:42
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; G01N33/74; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/805; Y10S436/815; Y10S436/817; Y10S436/826
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
100 words
A method of performing an immunoassay, which method comprises the steps of: i. incubating an aqueous mixture of the antigen to be assayed, a labelled version of the antigen, and a first antibody for the antigen in an amount insufficient to react with all the antigen, and ii. effecting precipitation of a first antibody-antigen complex by the addition to the aqueous mixture of an aqueous solution of a second antibody which reacts with the said first antibody-antigen complex, so as to form a precipitate, characterized in that said second antibody is artificially colored, so as to form a colored precipitate.
Claim 2
Independent Claim
100 words
A method of performing an immunoassay, which method comprises the steps of: i. incubating an aqueous mixture of the antigen to be assayed, a labelled version of the antigen, and a first antibody for the antigen in an amount insufficient to react with all the antigen, and ii. effecting precipitation of a first antibody-antigen complex by the addition to the aqueous mixture of an aqueous solution of a second antibody which reacts with the said first antibody-antigen complex, so as to form a precipitate, characterized in that said second antibody is artificially colored, so as to form a colored precipitate.
Claim 3
Dependent Claim
26 words
A method as claimed in claim 1, wherein the antigen to be assayed is human growth hormone, thyroid stimulating hormone, luteinising hormone or follicle stimulating hormone.
Claim 4
Dependent Claim
24 words
Method as claimed in claim 1 wherein the labelled version of the antigen is labelled by means of a radioactive isotope or an enzyme.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-12 | 1980-04-12 | 1980-10-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-12 | 1984-04-12 | 1984-10-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-12 | 1988-04-12 | 1988-10-12 | $7700.00 | Window Closed / Lapsed |