US3716632A
Expired / Lapsed
US
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Test composition and method for the in vitro determination of thyroid function
Assignee: Bayer Corp
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Filing: 1970-04-27
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Granted: 1973-02-13
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Expires: 1990-04-27
Abstract
Abstract The thyroid hormone content of blood serum can be readily determined by using an in vitro radioactive thyroid function test system containing a ''''protein error'''' pH indicator such as bromcresol purple. Such a ''''protein error'''' indicator provides color and detects the presence of the test sample, inhibits the formation of free iodide and decreases the adsorption of radioactive tagged thyroid substance to the walls of the container enclosing same.
Inventors
- 👤 M Fader
- 👤 J Christner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 11:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/826
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
60 words
In a test composition for determining thyroid function comprising a radioactive thyroid hormone and a buffer for establishing the pH of the composition in a predetermined range, the improvement which comprises adding to the composition a color responsive amount of a protein error type pH indicator which changes color in response to protein at the pH of the said buffer.
Claim 2
Dependent Claim
60 words
In a test composition for determining thyroid function comprising a radioactive thyroid hormone and a buffer for establishing the pH of the composition in a predetermined range, the improvement which comprises adding to the composition a color responsive amount of a protein error type pH indicator which changes color in response to protein at the pH of the said buffer.
Claim 3
Dependent Claim
22 words
A test composition as in claim 1 wherein the buffer is used to maintain the pH of the composition at about 5.
Claim 4
Dependent Claim
30 words
A test composition as in claim 1 wherein the indicator is selected from the group consisting of bromcresol purple, chlorphenol red, tetrabromphenol blue, bromphenol blue, bromcresol green and bromthymol blue.
Claim 5
Dependent Claim
13 words
A test composition as in claim 1 wherein the indicator is bromcresol purple.
Claim 6
Dependent Claim
13 words
A test composition as in claim 1 wherein the indicator is bromthymol blue.
Claim 7
Dependent Claim
23 words
A test composition as in claim 1 wherein the indicator is present in a concentration of about 60 micrograms per milliliter of composition.
Claim 8
Dependent Claim
13 words
A test composition as in claim 1 wherein the thyroid substance is triiodothyronine.
Claim 9
Dependent Claim
73 words
In a method for determining thyroid function wherein blood serum is Added to a composition comprising a radioactive tagged thyroid hormone and a buffer for establishing the pH of the composition in a predetermined range, the improvement which comprises the addition of a color responsive amount of a protein error type pH indicator which changes color in response to protein at the pH of the buffer prior to the addition of the serum.
Claim 10
Dependent Claim
73 words
In a method for determining thyroid function wherein blood serum is Added to a composition comprising a radioactive tagged thyroid hormone and a buffer for establishing the pH of the composition in a predetermined range, the improvement which comprises the addition of a color responsive amount of a protein error type pH indicator which changes color in response to protein at the pH of the buffer prior to the addition of the serum.
Claim 11
Dependent Claim
29 words
A method as in claim 8 wherein the indicator is selected from the group consisting of bromcresol purple, chlorphenol red, tetrabromphenol blue, bromphenol blue, bromcresol green and bromthymol blue.
Claim 12
Dependent Claim
12 words
A method as in claim 8 wherein the indicator is bromcresol purple.
Claim 13
Dependent Claim
22 words
A method as in claim 8 wherein the indicator is present in a concentration of about 60 micrograms per milliliter of composition.
Claim 14
Dependent Claim
12 words
A method as in claim 8 wherein the thyroid substance is triiodothyronine.
Claim 15
Independent Claim
39 words
A method of inhibiting the adsorption of radioactive triiodothyronine from an aqueous solution thereof on the walls of a polyethylene container which comprises adding to said aqueous solution an adsorption inhibiting amount of a protein error type pH indicator.
Claim 16
Independent Claim
39 words
A method of inhibiting the adsorption of radioactive triiodothyronine from an aqueous solution thereof on the walls of a polyethylene container which comprises adding to said aqueous solution an adsorption inhibiting amount of a protein error type pH indicator.
Claim 17
Dependent Claim
29 words
A method as in claim 13 wherein the indicator is selected from the group consisting of bromcresol purple, chlorphenol red, tetrabromphenol blue, bromphenol blue, bromcresol green and bromthymol blue.
Claim 18
Dependent Claim
12 words
A method as in claim 13 wherein the indicator is bromthymol blue.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-02-13 | 1976-08-14 | 1977-02-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-02-13 | 1980-08-14 | 1981-02-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-02-13 | 1984-08-14 | 1985-02-13 | $7700.00 | Window Closed / Lapsed |