Gentamicin assay and products therefor

Claim 2

A product comprising: a gentamicin-protein conjugate adsorbed on a solid support, said conjugate being crosslinked by a crosslinking agent to increase the stability thereof.

Claim 3

The product of claim 1 wherein the crosslinking agent is a dialdehyde.

Claim 4

An assay for gentamicin by agglutination wherein gentamicin-protein conjugate adsorbed on a solid particulate support is contacted with gentamicin antibody and sample, the improvement comprising: said conjugate on said solid particulate support being the product of claim 1.

Claim 5

The product of claim 1 wherein the solid support is polymer particles.

Claim 6

The product of claim 4 wherein the polymer is polystyrene.

Claim 7

The product of claim 1 wherein the crosslinking agent is glutaraldehyde.

Claim 8

The product of claim 6 wherein the support is polystyrene particles.

Claim 9

The product of claim 1 wherein the surface of the solid support also has a protein adsorbed thereon, said conjugate and said protein being formed into a crosslinked network by the crosslinking agent.

Claim 10

The product of claim 8 wherein the support is polymer particles.

Claim 11

The product of claim 8 wherein the crosslinking agent is a dialdehyde.

Claim 12

The product of claim 10 wherein the protein adsorbed on the surface of the solid support is ovalbumin, the crosslinking agent is glutaraldehyde and the support is polystyrene particles.

Claim 13

A product comprising: a conjugate of gentamicin and orosomucoid, said conjugate being supported on a solid support.

Claim 14

A product comprising: a conjugate of gentamicin and orosomucoid, said conjugate being supported on a solid support.

Claim 15

The product of claim 12 wherein the solid support is a particulate support.

Claim 16

In an agglutination assay for gentamicin wherein supported gentamicin is contacted with a sample and antibody to gentamicin, the improvement comprising: said supported gentamicin being the product of claim 13.

Claim 17

The product of claim 13 wherein the conjugate is crosslinked by a crosslinking agent to increase the stability thereof.

Claim 18

The product of claim 15 wherein the crosslinking agent is glutaraldehyde.

Claim 19

The product of claim 16 wherein the solid support is polystyrene particles, said solid support having a protein adsorbed thereon, said crosslinking agent forming the protein and conjugate into a crosslinked network.

Claim 20

In an assay for gentamicin by agglutination wherein gentamicin-protein conjugate adsorbed on a solid particulate support is contacted with gentamicin antibody and sample, the improvement comprising: said conjugate on said solid particulate support being the product of claim 16.

Claim 21

A kit for the assay of gentamicin by an agglutination procedure, comprising: a gentamicin-protein conjugate adsorbed on a solid particulate support, said conjugate being crosslinked by a crosslinking agent to increase the stability thereof; and antisera to gentamicin.

Claim 22

A kit for the assay of gentamicin by an agglutination procedure, comprising: a gentamicin-protein conjugate adsorbed on a solid particulate support, said conjugate being crosslinked by a crosslinking agent to increase the stability thereof; and antisera to gentamicin.

Claim 23

The kit of claim 19 wherein said anti-sera to gentamicin was produced by an immunogen comprised of gentamicin conjugated to a protein other than the protein of the gentamicin-protein conjugate adsorbed on the solid particulate support.

Claim 24

The kit of claim 20 wherein the protein of the supported gentamicin-protein conjugate is orosomucoid.

Claim 25

The kit of claim 21 wherein the protein of the immunogen is bovine serum albumin.

Claim 26

The kit of claim 19 and further comprising gentamicin standards.

Claim 27

The kit of claim 23 wherein the particulate support is a polymer.

Claim 28

The kit of claim 24 wherein the crosslinking agent is glutaraldehyde.

Claim 29

The kit of claim 25 wherein the surface of the solid support also has a protein adsorbed thereon, said conjugate and adsorbed protein being formed into a crosslinked network by the crosslinking agent.

Claim 30

The kit of claim 26 wherein the particles are polystyrene particles.

Claim 31

The kit of claim 27 wherein the protein of the conjugate is orosomucoid.

Claim 32

An improved card assay kit for determination of gentamicin by an agglutination procedure, comprising: a gentamicin-protein conjugate comprised of orosomucoid as the protein supported on a solid particulate support; an antibody to gentamicin; and an assay card, said assay card including a plurality of separated marked areas for receiving serial dilutions of a gentamicin sample and a plurality of separated marked areas for receiving gentamicin standard, whereby serial dilution of a sample and assay thereof can be conducted on the card.

Claim 33

An improved card assay kit for determination of gentamicin by an agglutination procedure, comprising: a gentamicin-protein conjugate comprised of orosomucoid as the protein supported on a solid particulate support; an antibody to gentamicin; and an assay card, said assay card including a plurality of separated marked areas for receiving serial dilutions of a gentamicin sample and a plurality of separated marked areas for receiving gentamicin standard, whereby serial dilution of a sample and assay thereof can be conducted on the card.

Claim 34

The kit of claim 29 and further comprising gentamicin standard.

Claim 35

The kit of claim 30 wherein the conjugate is adsorbed on the solid support, said conjugate being cross-linked by a crosslinking agent to increase the stability thereof.

Claim 36

The kit of claim 31 wherein the crosslinking agent is glutaraldehyde.

Claim 37

The kit of claim 32 wherein the particulate support is polystyrene.

Claim 38

The kit of claim 33 wherein the particulate support has a protein adsorbed on the surface thereof, said conjugate and adsorbed protein being formed in a crosslinked network by the crosslinking agent.

Claim 39

An improved card assay kit for determination of gentamicin by an agglutination procedure, comprising: a gentamicin-protein conjugate supported on a solid particulate support; an antibody to gentamicin; gentamicin standard; and an assay card, said assay card having a surface including a sample portion and a standard portion, said sample portion being comprised of at least one series of separated marked serial dilution areas for receiving at least one series of serial dilutions of a gentamicin sample, each of which has an indicia of the dilution, said standard portion including at least two separated marked standard areas for receiving gentamicin standard of different gentamicin concentration, each having an indicia of the gentamicin concentration, whereby a quantitative determination of gentamicin in a sample can be determined directly on the card by serial dilution of sample on the card, adding antibody to gentamicin and the gentamicin-protein conjugate to the serial diluted gentamicin samples and gentamicin standards and determining the lowest standard concentration and the highest dilution at which agglutination is inhibited.

Claim 40

An improved card assay kit for determination of gentamicin by an agglutination procedure, comprising: a gentamicin-protein conjugate supported on a solid particulate support; an antibody to gentamicin; gentamicin standard; and an assay card, said assay card having a surface including a sample portion and a standard portion, said sample portion being comprised of at least one series of separated marked serial dilution areas for receiving at least one series of serial dilutions of a gentamicin sample, each of which has an indicia of the dilution, said standard portion including at least two separated marked standard areas for receiving gentamicin standard of different gentamicin concentration, each having an indicia of the gentamicin concentration, whereby a quantitative determination of gentamicin in a sample can be determined directly on the card by serial dilution of sample on the card, adding antibody to gentamicin and the gentamicin-protein conjugate to the serial diluted gentamicin samples and gentamicin standards and determining the lowest standard concentration and the highest dilution at which agglutination is inhibited.