Patent US4302537A successfully refreshed from Google Patents.
US4302537A
Expired / Lapsed
US
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Reagent and method for the determination of peroxidase
Assignee: Roche Diagnostics GmbH
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Filing: 1980-02-11
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Granted: 1981-11-24
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Expires: 2000-02-11
Abstract
Abstract The present invention provides a method for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide of the formula ##STR1## is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.
Inventors
- 👤 Karl-Dietrich Gundermann
- 👤 Karl Wulff
- 👤 Fritz Staehler
- 👤 Hans-Ralf Linke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:10
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/968
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
52 words
Method for the determination of peroxidase in a sample, which method comprises contacting the sample with a peroxy compound selected from hydrogen peroxide or an organic hydroperoxide and 7-dimethyl-aminonaphthaline-1,2-dicarboxylic acid hydrozine of the formula ##STR3## and measuring the emission of light as a measure of the peroxidase initially present in the sample.
Claim 2
Dependent Claim
52 words
Method for the determination of peroxidase in a sample, which method comprises contacting the sample with a peroxy compound selected from hydrogen peroxide or an organic hydroperoxide and 7-dimethyl-aminonaphthaline-1,2-dicarboxylic acid hydrozine of the formula ##STR3## and measuring the emission of light as a measure of the peroxidase initially present in the sample.
Claim 3
Dependent Claim
87 words
Method as claimed in claim 1 for the determination of haptene by way of a peroxidase determination, which process comprises before the step of adding said acid hydrazide to said test sample, adding a known amount of peroxide marked haptene to a sample containing an unknown amount of haptene, contacting the sample with a specific anti-body of the haptene bound to a solid carrier, separating the solid phase from the liquid phase, and selecting one of said solid phase or said liquid phase as said test sample.
Claim 4
Dependent Claim
20 words
Method as claimed in claim 1 wherein the amount of light emitted in a definite interval of time is measured.
Claim 5
Dependent Claim
15 words
Method as claimed in claim 1 carried out at a pH of 6 to 9.
Claim 6
Dependent Claim
15 words
Method as claimed in claim 4 carried out at a pH of 7.5 to 8.5.
Claim 7
Dependent Claim
23 words
Method as claimed in claim 4 wherein the pH is adjusted with a buffer substance with a concentration of 10 to 1000 mMol/liter.
Claim 8
Dependent Claim
16 words
Method as claimed in claim 1 wherein a potassium phosphate or glycine buffer means is used.
Claim 9
Dependent Claim
32 words
Method as claimed in claim 1 wherein the step of contacting the sample with the acid hydrazide further comprises simultaneously contacting the acid hydrazide with 10 to 200 μMol/liter of hydrogen peroxide.
Claim 10
Dependent Claim
16 words
Method as claimed in claim 1 wherein 0.1 to 100 μMol/liter 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide are used.
Claim 11
Dependent Claim
18 words
Method as claimed in claim 1 carried out at a temperature of from about 20° to 37° C.
Claim 12
Dependent Claim
17 words
Method as claimed in claim 10 carried out at a temperature of from 22° to 30° C.
Claim 13
Dependent Claim
24 words
Reagent for the determination of peroxidase comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.
Claim 14
Dependent Claim
24 words
Reagent for the determination of peroxidase comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.
Claim 15
Dependent Claim
31 words
Reagent as claimed in claim 12 which comprises 0.1 to 100 μMol/liter 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide; 50 to 500 mMol/liter potassium phosphate or glycine buffer; and 10 to 200 μMol/liter hydrogen peroxide.
Claim 16
Dependent Claim
18 words
Reagent as claimed in claim 13 additionally containing 0.01 to 1 mMol per liter of a sequestering agent.
Claim 17
Dependent Claim
13 words
Reagent as claimed in claim 14 wherein the sequestering agent is ethylenediaminetetraacetic acid.
Claim 18
Dependent Claim
20 words
Reagent as claimed in claim 12 additionally comprising a haptene marked with peroxidase and carrier-bound specific antibodies against the haptene.
Claim 19
Dependent Claim
11 words
Reagent as claimed in claim 12 additionally comprising a stabilizing agent.
Claim 20
Dependent Claim
16 words
Reagent as claimed in claim 17 wherein said stabilizing agent is serum albumen or a carbohydrate.
Claim 21
Dependent Claim
22 words
Reagent as claimed in claim 12 wherein the system providing hydrogen peroxide is hydrogen peroxide or a substance which liberates hydrogen peroxide.
Claim 22
Dependent Claim
26 words
Reagent as claimed in claim 19 wherein the substance which liberates hydrogen peroxide is urea perhydrate, an organic hydroperoxide or a solid salt of hydrogen peroxide.
References Cited (Backward Citations)
US3654179A
Cited
US3986833A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-23 | 1985-05-25 | 1985-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-23 | 1989-05-25 | 1989-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-23 | 1993-05-25 | 1993-11-24 | $7700.00 | Window Closed / Lapsed |