US4296201A
Expired / Lapsed
US
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Methods for the detection of antimitochondrial and antinuclear antibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1979-08-16
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Granted: 1981-10-20
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Expires: 1999-08-16
Abstract
Abstract What is disclosed are methods for the detection of antimitochondrial and antinuclear antibodies employing hypernephroma cells as a diagnostic agent.
Inventors
- 👤 Wolfgang Ax
- 👤 Hartwig W. Bauer
- 👤 Hans-Harald Sedlacek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; G01N33/564; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
111 words
A method for testing for the presence of anti-mitochondrial antibodies which comprises coating air-dried unfixed hypernephroma cells with an isotonic salt solution of the antibody to be tested, incubating the coated cells for 10 minutes to 2 hours in a moist chamber, then washing the cells with an isotonic salt solution and coating them with a fluorescently-labeled antibody directed against antigen-determinants of the antibody sought for, incubating the cells for 10 minutes to 2 hours in a moist chamber, washing at least once with demineralized water, and examining the cells by immunofluorescence microscopy, whereby fluorescence of the mitochondria of the hypernephroma cells is considered a positive finding of said anti-mitochondrial antibodies.
Claim 2
Independent Claim
111 words
A method for testing for the presence of anti-mitochondrial antibodies which comprises coating air-dried unfixed hypernephroma cells with an isotonic salt solution of the antibody to be tested, incubating the coated cells for 10 minutes to 2 hours in a moist chamber, then washing the cells with an isotonic salt solution and coating them with a fluorescently-labeled antibody directed against antigen-determinants of the antibody sought for, incubating the cells for 10 minutes to 2 hours in a moist chamber, washing at least once with demineralized water, and examining the cells by immunofluorescence microscopy, whereby fluorescence of the mitochondria of the hypernephroma cells is considered a positive finding of said anti-mitochondrial antibodies.
Claim 3
Independent Claim
117 words
A method for testing for the presence of anti-nuclear antibodies which comprises coating fixed hypernephroma cells with an isotonic salt solution of the antibody to be tested, incubating the coated cells for 10 minutes to 2 hours in a moist chamber, then washing the cells with an isotonic salt solution and coating them with a fluorescently-labeled antibody directed against antigen-determinants of the antibody sought for, incubating for 10 minutes to 2 hours in a moist chamber, washing at least once with demineralized water, and examining the cells by immunofluorescence microscopy, whereby a homogeneous or differentiated spotting of the cell nuclei or fluorescence of the nuclear membrane or nucleoli is considered a positive finding of said anti-nuclear antibodies.
Claim 4
Independent Claim
117 words
A method for testing for the presence of anti-nuclear antibodies which comprises coating fixed hypernephroma cells with an isotonic salt solution of the antibody to be tested, incubating the coated cells for 10 minutes to 2 hours in a moist chamber, then washing the cells with an isotonic salt solution and coating them with a fluorescently-labeled antibody directed against antigen-determinants of the antibody sought for, incubating for 10 minutes to 2 hours in a moist chamber, washing at least once with demineralized water, and examining the cells by immunofluorescence microscopy, whereby a homogeneous or differentiated spotting of the cell nuclei or fluorescence of the nuclear membrane or nucleoli is considered a positive finding of said anti-nuclear antibodies.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-19 | 1985-04-20 | 1985-10-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-19 | 1989-04-20 | 1989-10-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-19 | 1993-04-20 | 1993-10-20 | $7700.00 | Window Closed / Lapsed |