US4293538A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Assay for hepatitis A antigen
Assignee: Merck and Co Inc
•
Filing: 1979-04-20
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Granted: 1981-10-06
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Expires: 1999-04-20
Abstract
Abstract Liquid containing hepatitis A antibody is adsorbed on a surface. The surface is then coated with a proteinaceous material and incubated in the presence of the sample to form a complex of hepatitis A antigen and hepatitis A antibody. The complex is incubated again in the presence of excess radioactively labelled hepatitis A antibody, and the resulting radionuclide is measured.
Inventors
- 👤 William J. McAleer
- 👤 William M. Hurni
- 👤 William J. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 04:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5768; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/82
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
102 words
An assay for detection of hepatitis A antigen in a sample which comprises the steps of: contacting a surface having hepatitis A antibody adsorbed thereto as the first layer with a proteinaceous material capable of being adsorbed to the surface; contacting the surface having adsorbed thereto the hepatitis A antibody and proteinaceous material with a sample containing hepatitis A antigen; incubating the surface under conditions effective to form a complex of hepatitis A antigen and hepatitis A antibody; incubating the surface in the presence of an excess amount of radioactively labelled hepatitis A antibody, and measuring the resulting radionuclide on the surface.
Claim 2
Dependent Claim
102 words
An assay for detection of hepatitis A antigen in a sample which comprises the steps of: contacting a surface having hepatitis A antibody adsorbed thereto as the first layer with a proteinaceous material capable of being adsorbed to the surface; contacting the surface having adsorbed thereto the hepatitis A antibody and proteinaceous material with a sample containing hepatitis A antigen; incubating the surface under conditions effective to form a complex of hepatitis A antigen and hepatitis A antibody; incubating the surface in the presence of an excess amount of radioactively labelled hepatitis A antibody, and measuring the resulting radionuclide on the surface.
Claim 3
Dependent Claim
47 words
A hepatitis A antigen antibody radionuclide comprising a surface having adhered thereto as the first layer hepatitis A antibody, at least part of the antibody being complexed with hepatitis A antigen and at least part of the hepatitis A antigen being complexed with a radioactively labelled material.
Claim 4
Dependent Claim
47 words
A hepatitis A antigen antibody radionuclide comprising a surface having adhered thereto as the first layer hepatitis A antibody, at least part of the antibody being complexed with hepatitis A antigen and at least part of the hepatitis A antigen being complexed with a radioactively labelled material.
Claim 5
Dependent Claim
15 words
A radionuclide according to claim 2 wherein the radioactively labelled material is hepatitis A antibody.
Claim 6
Dependent Claim
31 words
A diagnostic assay for hepatitis A antigen comprising a sample containing hepatitis A antigen and a surface having adsorbed thereto as the first layer hepatitis A antibody and a proteinaceous material.
Claim 7
Dependent Claim
31 words
A diagnostic assay for hepatitis A antigen comprising a sample containing hepatitis A antigen and a surface having adsorbed thereto as the first layer hepatitis A antibody and a proteinaceous material.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-05 | 1985-04-06 | 1985-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-05 | 1989-04-06 | 1989-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-05 | 1993-04-06 | 1993-10-06 | $7700.00 | Window Closed / Lapsed |